LOCUS       Exported                9858 bp ds-DNA     circular SYN 17-JAN-2023
DEFINITION  synthetic DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported  30-JUN-2022 from NEBuilder https://nebuilder.neb.com
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase = 
            overlap)
            FSW-H3MD-Vector_fwd: ATCGATAATCAACCTCTGGATTACAAAATTTG
            FSW-H3MD-Vector_fwd 3'Tm: 64.7  3'Ta: 65.7
            FSW-H3MD-Vector_rev: CATACGCAGGGCCCTCTG
            FSW-H3MD-Vector_rev 3'Tm: 68.6  3'Ta: 65.7
            hSyn1-tdTomato_fwd: tgcagagggccctgcgtatgAGTGCAAGTGGGTTTTAG
            hSyn1-tdTomato_fwd 3'Tm: 59.3  3'Ta: 57.2
            hSyn1-tdTomato_rev: tccagaggttgattatcgatAAGCTTGATAAACCCTTG
            hSyn1-tdTomato_rev 3'Tm: 56.2  3'Ta: 57.2
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(1966..9858,1..1965)
                     /organism="synthetic DNA construct"
                     /plasmid="FSW-tdTomato"
                     /mol_type="other DNA"
     gene            1..1965
                     /label=hSyn1-tdTomato
                     /note="hSyn1-tdTomato"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      498..507
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             504..1931
                     /codon_start=1
                     /label=tdTomato
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(1948..1985)
                     /label=hSyn1-tdTomato_rev
                     /note="hSyn1-tdTomato_rev"
                     /note="gene-specific Tm: 56.2  Ta: 57.2"
                     /note="overlap: tccagaggttgattatcgat"
                     /note="gene-specific primer: AAGCTTGATAAACCCTTG"
     gene            1966..9858
                     /label=FSW-H3MD-Vector
                     /note="FSW-H3MD-Vector"
     primer_bind     1966..1997
                     /label=FSW-H3MD-Vector_fwd
                     /note="FSW-H3MD-Vector_fwd"
                     /note="gene-specific Tm: 64.7  Ta: 65.7"
                     /note="gene-specific primer: 
                     ATCGATAATCAACCTCTGGATTACAAAATTTG"
     misc_feature    1972..2560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2443..2454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2563..2579)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3085..3265
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3297..3521
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3297..3504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3567..3995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4009..4338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4189..4324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4386..4433
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4452..4826
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4956..5077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5150..5166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5174..5204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5219..5240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5528..6116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6287..7147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7148..7252)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7474..7853
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7855..8053
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8071..8251
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8298..8423
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8916..9149
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9334..9378
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9676..9793
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     join(9839..9858,1..18)
                     /label=hSyn1-tdTomato_fwd
                     /note="hSyn1-tdTomato_fwd"
                     /note="gene-specific Tm: 59.3  Ta: 57.2"
                     /note="overlap: tgcagagggccctgcgtatg"
                     /note="gene-specific primer: AGTGCAAGTGGGTTTTAG"
     primer_bind     complement(9841..9858)
                     /label=FSW-H3MD-Vector_rev
                     /note="FSW-H3MD-Vector_rev"
                     /note="gene-specific Tm: 68.6  Ta: 65.7"
                     /note="gene-specific primer: CATACGCAGGGCCCTCTG"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagac gcgtggacgg tacccttcag gtcgacgtcg
      481 gttggcgcgc cgctagcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca
      541 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      601 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      661 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
      721 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
      781 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
      841 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
      901 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
      961 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
     1021 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
     1081 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     1141 aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggt accggcagca
     1201 ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc gtgattaagg
     1261 agtttatgag gtttaaagtc aggatggagg gcagcatgaa cgggcacgag tttgagattg
     1321 aaggagaggg ggaagggagg ccctatgagg gcacccagac agcaaagctg aaagtgacaa
     1381 agggcggccc tctgccattc gcatgggaca tcctgtctcc tcagtttatg tatggaagta
     1441 aagcctacgt gaaacaccct gctgacattc cagattacaa gaaactgagc ttcccagagg
     1501 ggtttaagtg ggaaagagtg atgaacttcg aagacggggg cctggtgact gtcacccagg
     1561 acagctctct gcaggacggc actctgatct ataaggtgaa aatgcgcggc accaattttc
     1621 cccctgatgg cccagtcatg cagaagaaga caatgggctg ggaggcttca acagaaaggc
     1681 tgtaccccag agacggcgtg ctgaagggag agatccacca ggcactgaag ctgaaagatg
     1741 gagggcatta tctggtcgaa ttcaaaacca tctacatggc caagaaaccc gtgcagctgc
     1801 ctggctacta ttacgtcgac acaaagctgg atattactag ccataacgaa gactacacca
     1861 ttgtggagca gtatgagcgg agcgagggga ggcaccatct gtttctgtac ggcatggacg
     1921 agctgtacaa gtaagctagc ggcgcgccaa gggtttatca agcttatcga taatcaacct
     1981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2341 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2521 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     2581 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     2641 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     2701 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     2761 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     2821 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     2881 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     2941 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     3001 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     3061 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     3121 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3181 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3241 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     3301 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3361 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3421 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3481 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3541 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3781 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3841 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3901 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3961 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4021 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4081 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4141 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4201 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4261 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4321 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     4381 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4441 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     4501 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     4561 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     4621 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     4681 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     4741 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     4801 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     4861 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     4921 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     4981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5101 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7261 cccgaaaagt gccacctgac actacaatct gctctgatgc cgcatagtta agccagtatc
     7321 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7381 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7441 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7501 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7561 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7621 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7681 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7741 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7801 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7861 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7921 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7981 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     8041 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8221 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8281 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8341 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8401 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8461 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8521 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8581 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8641 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8701 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8761 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8821 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8881 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8941 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     9001 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9061 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9121 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9181 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9241 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     9301 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9361 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9421 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9481 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9541 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     9601 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     9661 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9721 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9781 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaactg
     9841 cagagggccc tgcgtatg
//
