LOCUS       Exported                2528 bp ds-DNA     circular SYN 12-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 19, 2023 from SnapGene 5.1.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             389..535
                     /codon_start=1
                     /label=M7
                     /label=Lck
                     /note="color: #993366"
                     /translation="RYPGIHLQHSGSGSLPTWSTPVQPMALIVLGGSGSGSGSGTRQVD
                     PLES"
     rep_origin      complement(725..1392)
                     /direction=LEFT
                     /label=Col E1 origin
                     /label=Col\E1\origin
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     CDS             complement(1540..2400)
                     /codon_start=1
                     /label=AmpR
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccacg gtacccgggg atccacctgc aacactccgg
      421 atcaggatct ctgcctacct ggtctacacc tgtgcagccc atggctctga tcgtgcttgg
      481 aggctccgga tcaggatccg gcagtggtac aaggcaggtg gatcctctag agtcgtttta
      541 attaatggag cacaagactg gcctcatggg ccttccgctc actgcccgct ttccagtcgg
      601 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
      661 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
      721 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1681 agaaccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2521 agtgccac
//
