LOCUS       Exported                9842 bp ds-DNA     circular UNK 17-JAN-2023
DEFINITION  Plasmid was generated by QUEEN v1.2.0 
            (https://github.com/yachielab/QUEEN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="."
                     /note="/building_history_39254=QUEEN.dna_dict['pLV_CS_552']
                     –=–QUE 
                     EN(record='https://benchling.com/s/seq-KzYdG9UVFpnuTv5G3FIP
                      ',–dbtype='benchling',–product='pLV_CS_038')"
                     /note="/building_history_39254="
                     /note="/building_history_39254=pLV_CS_691-6YXE5YG1XZ6M2MCH5
                     6HLVND
                     0"
                     /note="/building_history_39255=QUEEN.dna_dict['pLV_CS_553']
                     –=–QUE 
                     EN(record='https://benchling.com/s/seq-AXIf1rdVlbb2QDPoOudT
                      ',–dbtype='benchling',–product='pLV_CS_043')"
                     /note="/building_history_39255="
                     /note="/building_history_39255=pLV_CS_691-B0MZH81LHT8E7IA48
                     TWAMG8
                     P"
                     /note="/building_history_49631=QUEEN.dna_dict['SI417_65']–=
                     –QUEEN 
                     (seq='TGAGTAAAGTTTGAAGCCATGAATTCCCTGTGTTCTGGCGGCAAACCCGTTG'
                      ,–product='SI417')"
                     /note="/building_history_49631="
                     /note="/building_history_49631=pLV_CS_691-59OS8ZCVDOOO2A2MR
                     FCLE3H
                     T"
                     /note="/building_history_49632=QUEEN.dna_dict['SI415_65']–=
                     –QUEEN 
                     (seq='TGGACGAGCTGTACAAGTAATTAATTAACTAGGTCTTGAAAGGAGTGGGAATT
                      GGC',–product='SI415')"
                     /note="/building_history_49632="
                     /note="/building_history_49632=pLV_CS_691-3LC6B9Z2KNTDCPTPL
                     CUTCNB
                     3"
                     /note="/building_history_54099=QUEEN.dna_dict['SI417_67']–=
                     –QUEEN 
                     (seq='TGAGTAAAGTTTGAAGCCATGAATTCCCTGTGTTCTGGCGGCAAACCCGTTG'
                      ,–product='SI417')"
                     /note="/building_history_54099="
                     /note="/building_history_54099=pLV_CS_691-BA88WFHCBGM92A2MR
                     FCLE3H
                     T"
                     /note="/building_history_54100=QUEEN.dna_dict['SI415_67']–=
                     –QUEEN 
                     (seq='TGGACGAGCTGTACAAGTAATTAATTAACTAGGTCTTGAAAGGAGTGGGAATT
                      GGC',–product='SI415')"
                     /note="/building_history_54100="
                     /note="/building_history_54100=pLV_CS_691-DDSA8WVFGDQLCPTPL
                     CUTCNB
                     3"
                     /note="/building_history_58075=QUEEN.dna_dict['SI415_72']–=
                     –QUEEN 
                     (seq='TGGACGAGCTGTACAAGTAATTAATTAACTAGGTCTTGAAAGGAGTGGGAATT
                      GGC',–product='SI415')"
                     /note="/building_history_58075="
                     /note="/building_history_58075=pLV_CS_691-5S19EZRWYRK4CPTPL
                     CUTCNB
                     3"
                     /note="/building_history_58076=QUEEN.dna_dict['SI417_72']–=
                     –QUEEN 
                     (seq='TGAGTAAAGTTTGAAGCCATGAATTCCCTGTGTTCTGGCGGCAAACCCGTTG'
                      ,–product='SI417')"
                     /note="/building_history_58076="
                     /note="/building_history_58076=pLV_CS_691-CS9Z63UHETN92A2MR
                     FCLE3H
                     T"
                     /note="/building_history_58194=QUEEN.dna_dict['SI417_73']–=
                     –QUEEN 
                     (seq='TGAGTAAAGTTTGAAGCCATGAATTCCCTGTGTTCTGGCGGCAAACCCGTTG'
                      ,–product='SI417')"
                     /note="/building_history_58194="
                     /note="/building_history_58194=pLV_CS_691-93SCRTJQDM1J2A2MR
                     FCLE3H
                     T"
                     /note="/building_history_58195=QUEEN.dna_dict['SI415_73']–=
                     –QUEEN 
                     (seq='TGGACGAGCTGTACAAGTAATTAATTAACTAGGTCTTGAAAGGAGTGGGAATT
                      GGC',–product='SI415')"
                     /note="/building_history_58195="
                     /note="/building_history_58195=pLV_CS_691-1LLOL4DZIPD4CPTPL
                     CUTCNB
                     3"
                     /note="/building_history_58859=QUEEN.queried_features_dict[
                     'pLV_C 
                     S_043_f16561']–=–QUEEN.dna_dict['pLV_CS_553'].searchsequenc
                      e(query=cs.lib['PacI'].cutsite,–product='re1site')"
                     /note="/building_history_58859=query: 
                     cs.lib['PacI'].cutsite;
                     _source: tmpsyxktm3s"
                     /note="/building_history_58859=pLV_CS_691-8JJMF4O1A5MZBUF7B
                     OP99F0
                     U"
                     /note="/building_history_58860=QUEEN.queried_features_dict[
                     'pLV_C 
                     S_043_f16562']–=–QUEEN.dna_dict['pLV_CS_553'].searchsequenc
                      e(query=cs.lib['EcoRI'].cutsite,–product='re2site')"
                     /note="/building_history_58860=query: 
                     cs.lib['EcoRI'].cutsite;
                     _source: tmpsyxktm3s"
                     /note="/building_history_58860=pLV_CS_691-BUAQGQF1YT61ETS0C
                     7Q02EU
                     Y"
                     /note="/building_history_58861=QUEEN.dna_dict['digested_bac
                     kbone_ 
                     469']–=–cropdna(QUEEN.dna_dict['pLV_CS_553'],–start=QUEEN.q
                      
                     ueried_features_dict['pLV_CS_043_f16562'][0],–end=QUEEN.que
                      
                     ried_features_dict['pLV_CS_043_f16561'][0],–product='digest
                      
                     ed_backbone',–process_name='Digest–backbone',–process_descr
                      
                     iption='Vector–backbone–plasmid–was–digested–with–two–disti
                      
                     nct–restriction–enzymes–(see–Table–S2–for–details)–with–rCu
                      
                     tSmart–Buffer–(NEB–#B6004S)–at–37ºC,–then–size-selected–and
                      
                     –purified–using–GeneJET–Gel–Extraction–Kit–(Thermo–Fisher–S
                      cientific–#K0691).')"
                     /note="/building_history_58861=start: '5202/5206'; end:
                     '4493/4495'; _source: tmpsyxktm3s"
                     /note="/building_history_58861=pLV_CS_691-4N9HVDH1JR0653K3M
                     T8476Z
                     R"
                     /note="/building_history_58928=QUEEN.queried_features_dict[
                     'pLV_C 
                     S_038_f16620']–=–QUEEN.dna_dict['pLV_CS_552'].searchsequenc
                      
                     e(query=QUEEN.dna_dict['SI415_73'].seq[-56:],–product='matc
                      h')"
                     /note="/building_history_58928=query:
                     QUEEN.dna_dict['SI415_73'].seq[-56:]; _source: tmpsyxktm3s"
                     /note="/building_history_58928=pLV_CS_691-6KKSF81K9LDN9TZ5J
                     PFG8Z2
                     Z"
                     /note="/building_history_58937=QUEEN.queried_features_dict[
                     'pLV_C 
                     S_038_f16629']–=–QUEEN.dna_dict['pLV_CS_552'].searchsequenc
                      
                     e(query=QUEEN.dna_dict['SI417_73'].seq[-26:],–product='matc
                      h')"
                     /note="/building_history_58937=query:
                     QUEEN.dna_dict['SI417_73'].seq[-26:]; _source: tmpsyxktm3s"
                     /note="/building_history_58937=pLV_CS_691-1KJ1IR3AW8PLEUMOV
                     8IDV9D
                     O"
                     /note="/building_history_58939=QUEEN.dna_dict['extract1_684
                     ']–=–c 
                     ropdna(QUEEN.dna_dict['pLV_CS_552'],–start=QUEEN.queried_fe
                      
                     atures_dict['pLV_CS_038_f16620'][0].end,–end=QUEEN.queried_
                      
                     features_dict['pLV_CS_038_f16629'][0].start,–product='extra
                      
                     ct1',–process_name='Amplify–insert',–process_description='I
                      
                     nsert–fragments–were–amplified–by–Phusion–High-Fidelity–DNA
                      
                     –Polymerase–(NEB–#M0530)–using–forward–and–reverse–primers–
                      
                     and–1–ng–of–template–plasmid–(see–Table–S2–for–details)–acc
                      ording–to–the–manufacturer's–PCR–protocol.')"
                     /note="/building_history_58939=start: 5379; end: 5582; 
                     _source:
                     tmpsyxktm3s"
                     /note="/building_history_58939=pLV_CS_691-4NUXO9UEHN6UAC6FX
                     CCT6M0
                     N"
                     /note="/building_history_58941=QUEEN.dna_dict['PCR_product_
                     685']– 
                     =–modifyends(QUEEN.dna_dict['extract1_684'],–left=QUEEN.dna
                      
                     _dict['SI415_73'].seq,–right=QUEEN.dna_dict['SI417_73'].rcs
                      
                     eq,–product='PCR_product',–process_name='Amplify–insert',–p
                      
                     rocess_description='Insert–fragments–were–amplified–by–Phus
                      
                     ion–High-Fidelity–DNA–Polymerase–(NEB–#M0530)–using–forward
                      
                     –and–reverse–primers–and–1–ng–of–template–plasmid–(see–Tabl
                      
                     e–S2–for–details)–according–to–the–manufacturer's–PCR–proto
                      col.')"
                     /note="/building_history_58941=left: 
                     TGGACGAGCTGTACAAGTAATTAATTAACTAGGTCTTGAAAGGAGTGGGAATTGGC; 
                     right: 
                     CAACGGGTTTGCCGCCAGAACACAGGGAATTCATGGCTTCAAACTTTACTCA; 
                     leftobj: QUEEN.dna_dict['SI415_73'].seq; rightobj: 
                     QUEEN.dna_dict['SI417_73'].rcseq; _source: tmpsyxktm3s"
                     /note="/building_history_58941=pLV_CS_691-DQUC4HWMAFUH71GXX
                     S5VJBT
                     L"
                     /note="/building_history_59011=QUEEN.dna_dict['digested_bac
                     kbone_ 
                     715']–=–cropdna(QUEEN.dna_dict['digested_backbone_469'],–st
                      art='25/25',–end='9556/9556')"
                     /note="/building_history_59011=start: 25; end: 9556; 
                     _source:
                     tmpsyxktm3s"
                     /note="/building_history_59011=pLV_CS_691-B7N4HH745I46DIR0C
                     G6WIOC
                     1"
                     /note="/building_history_59012=QUEEN.dna_dict['pLV_CS_691']
                     –=–joi 
                     ndna(*[QUEEN.dna_dict['digested_backbone_715'],–QUEEN.dna_d
                      
                     ict['PCR_product_685']],–topology='circular',–product='pLV_
                      
                     CS_060',–process_name='Assemble',–process_description='High
                      
                     er–molar–ratios–of–insert–to–digested–backbone–fragments–we
                      
                     re–mixed–to–a–total–volume–of–20–µL–with–2–µL–of–5x–ISO–Buf
                      
                     fer,–0.2–µL–of–T5–Exonuclease–(NEB–#M0363L),–1–µL–of–Taq–DN
                      
                     A–Ligase–(NEB–#M0208S),–and–0.125–µL–of–Phusion–High-Fideli
                      
                     ty–DNA–Polymerase–(NEB–#M0530)–and–incubated–at–50ºC–for–1–
                      hour.')"
                     /note="/building_history_59012=topology: circular; _source:
                     tmpsyxktm3s"
                     /note="/building_history_59012=pLV_CS_691-7RPOQ0I4LH6926IMX
                     WBG7PU
                     H"
     misc_feature    344..464
                     /label=SV40-PA terminator
     misc_feature    1090..1278
                     /label=5' LTR
     misc_feature    1329..1466
                     /label=Psi
     misc_feature    1433..1786
                     /label=GAG
     misc_feature    1940..2238
                     /label=PRE
     misc_feature    2239..2655
                     /label=ENV
     misc_feature    2670..2787
                     /label=CPPT
     misc_feature    2826..2833
                     /label=SwaI
     gene            complement(2834..3544)
                     /label=mCherry
     misc_feature    3545..3550
                     /label=Kozak
     misc_feature    3551..3556
                     /label=HpaI
     misc_feature    complement(3557..3616)
                     /label=minCMV
     misc_feature    3617..3619
                     /label=PAM
     misc_feature    3620..3644
                     /label=c1
     misc_feature    3645..3646
                     /label=PAM
     misc_feature    3647..3652
                     /label=XbaI
     misc_feature    3653..3662
                     /label=linker
     misc_feature    3663..3668
                     /label=BmtI
     misc_feature    3669..3670
                     /label=PAM
     misc_feature    3671..3695
                     /label=c2
     misc_feature    3696..3698
                     /label=PAM
     misc_feature    3759..3764
                     /label=BstBI
     misc_feature    3765..3770
                     /label=Kozak
     gene            3771..4490
                     /label=EGFP
     misc_feature    4499..4755
                     /label=EF1a-core (SAM v2)
     misc_feature    4762..5151
                     /label=MCP
     misc_feature    4927..4929
                     /label=N55K
     misc_feature    5152..5157
                     /label=AfeI
     misc_feature    5158..5202
                     /label=Linker (GGS)5
     misc_feature    5203..5235
                     /label=NLS
     misc_feature    5236..5241
                     /label=BamHI
     misc_feature    5242..5793
                     /label=p65
     misc_feature    5794..5808
                     /label=GGGGS
     misc_feature    5809..6180
                     /label=HSF1
     CDS             6187..6243
                     /label=P2A
     CDS             6244..6840
                     /label=PuroR
     misc_feature    6851..7444
                     /label=WPRE
     misc_feature    7694..7882
                     /label=3' LTR
     primer_bind     complement(8005..8025)
                     /label=M13-rev
     misc_binding    complement(8031..8053)
                     /label=LacO
     promoter        complement(8057..8087)
                     /label=lac promoter
     rep_origin      complement(8393..9075)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(9173..9832)
                     /label=AmpR
ORIGIN
        1 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aactttgttt attgcagctt
      361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
      481 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
      541 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
      601 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
      661 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
      721 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
      781 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
      841 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
      901 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
      961 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     1021 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     1081 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1261 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1321 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1381 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1441 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1501 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1561 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1621 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1681 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1741 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     1801 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1861 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1921 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1981 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     2041 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     2101 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     2161 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     2221 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     2281 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     2341 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     2401 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2461 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2521 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2581 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2641 gattagtgaa cggatctcga cggtatcgcc tttaaaagaa aaggggggat tggggggtac
     2701 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2761 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2821 tatcgattta aatttacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
     2881 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
     2941 tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga
     3001 cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca
     3061 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
     3121 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
     3181 cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
     3241 tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg
     3301 cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg
     3361 cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
     3421 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
     3481 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
     3541 ccatggtggc gttaacgcga tctgacggtt cactaaacga gctctgctta tataggcctc
     3601 ccaccgtaca cgcctaccta ctgactgcag tctgagtctg acagcctcta gagtgttgta
     3661 aagctagcgg agcagagcta cgcactctac gctagaggta ggcgtgtacg gtgggaggcc
     3721 tatataagca gagctcgttt agtgaaccgt cagatcgctt cgaagccacc atggtgagca
     3781 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3841 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3901 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3961 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4021 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4081 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4141 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4201 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4261 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4321 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4381 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4441 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ttaattaact
     4501 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     4561 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
     4621 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     4681 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     4741 ccgccagaac acagggaatt catggcttca aactttactc agttcgtgct cgtggacaat
     4801 ggtgggacag gggatgtgac agtggctcct tctaatttcg ctaatggggt ggcagagtgg
     4861 atcagctcca actcacggag ccaggcctac aaggtgacat gcagcgtcag gcagtctagt
     4921 gcccagaaga gaaagtatac catcaaggtg gaggtcccca aagtggctac ccagacagtg
     4981 ggcggagtcg aactgcctgt cgccgcttgg aggtcctacc tgaacatgga gctcactatc
     5041 ccaattttcg ctaccaattc tgactgtgaa ctcatcgtga aggcaatgca ggggctcctc
     5101 aaagacggta atcctatccc ttccgccatc gccgctaact caggtatcta cagcgctgga
     5161 ggaggtggaa gcggaggagg aggaagcgga ggaggaggta gcggacctaa gaaaaagagg
     5221 aaggtggcgg ccgctggatc cccttcaggg cagatcagca accaggccct ggctctggcc
     5281 cctagctccg ctccagtgct ggcccagact atggtgccct ctagtgctat ggtgcctctg
     5341 gcccagccac ctgctccagc ccctgtgctg accccaggac caccccagtc actgagcgct
     5401 ccagtgccca agtctacaca ggccggcgag gggactctga gtgaagctct gctgcacctg
     5461 cagttcgacg ctgatgagga cctgggagct ctgctgggga acagcaccga tcccggagtg
     5521 ttcacagatc tggcctccgt ggacaactct gagtttcagc agctgctgaa tcagggcgtg
     5581 tccatgtctc atagtacagc cgaaccaatg ctgatggagt accccgaagc cattacccgg
     5641 ctggtgaccg gcagccagcg gccccccgac cccgctccaa ctcccctggg aaccagcggc
     5701 ctgcctaatg ggctgtccgg agatgaagac ttctcaagca tcgctgatat ggactttagt
     5761 gccctgctgt cacagatttc ctctagtggg cagggaggag gtggaagcgg cttcagcgtg
     5821 gacaccagtg ccctgctgga cctgttcagc ccctcggtga ccgtgcccga catgagcctg
     5881 cctgaccttg acagcagcct ggccagtatc caagagctcc tgtctcccca ggagcccccc
     5941 aggcctcccg aggcagagaa cagcagcccg gattcaggga agcagctggt gcactacaca
     6001 gcgcagccgc tgttcctgct ggaccccggc tccgtggaca ccgggagcaa cgacctgccg
     6061 gtgctgtttg agctgggaga gggctcctac ttctccgaag gggacggctt cgccgaggac
     6121 cccaccatct ccctgctgac aggctcggag cctcccaaag ccaaggaccc cactgtctcc
     6181 tgtacagcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     6241 ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6301 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6361 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6421 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6481 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6541 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6601 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     6661 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6721 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6781 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6841 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6901 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6961 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7021 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7081 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7141 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7201 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7261 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7321 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7381 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7441 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7501 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7561 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7621 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     7681 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7741 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7801 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7861 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7921 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttctagcgtt ttaccgtcga
     7981 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8041 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8101 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8161 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8221 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8281 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8341 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8401 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8461 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8521 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8581 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8641 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8701 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8761 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8821 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8881 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8941 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9001 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9061 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9841 aa
//
