LOCUS       Exported                8398 bp ds-DNA     circular UNK 17-JAN-2023
DEFINITION  Plasmid was generated by QUEEN v1.2.0 
            (https://github.com/yachielab/QUEEN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="."
                     /note="/building_history_39219=QUEEN.dna_dict['pLV_mCherry_
                     41']–= 
                     –QUEEN(record='https://benchling.com/s/seq-lyizd9Ah3jyfw9GF
                      wW9v',–dbtype='benchling',–product='pLV_mCherry')"
                     /note="/building_history_39219="
                     /note="/building_history_39219=pLV_CS_659-BU8DOBTNNTQQ1GAMF
                     JMLPUF
                     Q"
                     /note="/building_history_39220=QUEEN.dna_dict['pLVSIN_CMV_p
                     ur_12' 
                     ]–=–QUEEN(record='https://benchling.com/s/bc34hXWP',–dbtype
                      ='benchling',–product='pLVSIN_CMV_pur')"
                     /note="/building_history_39220="
                     /note="/building_history_39220=pLV_CS_659-E5BKN0PVEWIB1WMZ1
                     F9Q659
                     X"
                     /note="/building_history_45596=QUEEN.dna_dict['SI680_52']–=
                     –QUEEN 
                     (seq='AATTGGATCCTTACTTGTACAGCTCGTCCA',–product='SI680')"
                     /note="/building_history_45596="
                     /note="/building_history_45596=pLV_CS_659-47V0Y2TG3UTJ13GN8
                     7MC059
                     W"
                     /note="/building_history_45638=QUEEN.dna_dict['SI1295_66']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCGTGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1295')"
                     /note="/building_history_45638="
                     /note="/building_history_45638=pLV_CS_659-82EXCOBWOM2X3F6OT
                     BESP0V
                     Q"
                     /note="/building_history_45641=QUEEN.dna_dict['SI1298_44']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCATGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1298')"
                     /note="/building_history_45641="
                     /note="/building_history_45641=pLV_CS_659-5CJOYGMGAYXS40QID
                     8QU0Q7
                     4"
                     /note="/building_history_48119=QUEEN.dna_dict['SI680_53']–=
                     –QUEEN 
                     (seq='AATTGGATCCTTACTTGTACAGCTCGTCCA',–product='SI680')"
                     /note="/building_history_48119="
                     /note="/building_history_48119=pLV_CS_659-6DP0MFDM0PMY13GN8
                     7MC059
                     W"
                     /note="/building_history_48159=QUEEN.dna_dict['SI1293_65']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCGTGAGCAAGGGCGAGG',–prod
                      uct='SI1293')"
                     /note="/building_history_48159="
                     /note="/building_history_48159=pLV_CS_659-2L8HZVFM8OAV9XU6P
                     2MINST
                     A"
                     /note="/building_history_48161=QUEEN.dna_dict['SI1295_67']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCGTGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1295')"
                     /note="/building_history_48161="
                     /note="/building_history_48161=pLV_CS_659-LD11ULY73LZ83F6OT
                     BESP0V
                     Q"
                     /note="/building_history_48162=QUEEN.dna_dict['SI1296_45']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCATGAGCAAGGGCGAGG',–prod
                      uct='SI1296')"
                     /note="/building_history_48162="
                     /note="/building_history_48162=pLV_CS_659-IV8NPHFWJSTK5KO3I
                     JNYI5E
                     L"
                     /note="/building_history_48164=QUEEN.dna_dict['SI1298_45']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCATGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1298')"
                     /note="/building_history_48164="
                     /note="/building_history_48164=pLV_CS_659-BJE0WJI7PLVQ40QID
                     8QU0Q7
                     4"
                     /note="/building_history_48878=QUEEN.dna_dict['dna_12297']–
                     =–QUEE N(seq='GAGGATAACATGGCCATC')"
                     /note="/building_history_48878="
                     /note="/building_history_48878=pLV_CS_659-AJHD2SN4TGCFBDZNN
                     BNLPEG
                     8"
                     /note="/building_history_48888=QUEEN.dna_dict['dna_12299']–
                     =–QUEE N(seq='GAGGATAACATGGCCATC')"
                     /note="/building_history_48888="
                     /note="/building_history_48888=pLV_CS_659-DG4XO1EHCQESBDZNN
                     BNLPEG
                     8"
                     /note="/building_history_50889=QUEEN.dna_dict['SI680_55']–=
                     –QUEEN 
                     (seq='AATTGGATCCTTACTTGTACAGCTCGTCCA',–product='SI680')"
                     /note="/building_history_50889="
                     /note="/building_history_50889=pLV_CS_659-7SAN5V67JCWW13GN8
                     7MC059
                     W"
                     /note="/building_history_50931=QUEEN.dna_dict['SI1295_69']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCGTGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1295')"
                     /note="/building_history_50931="
                     /note="/building_history_50931=pLV_CS_659-B0LJEL6V2UL83F6OT
                     BESP0V
                     Q"
                     /note="/building_history_50934=QUEEN.dna_dict['SI1298_47']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCATGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1298')"
                     /note="/building_history_50934="
                     /note="/building_history_50934=pLV_CS_659-DMXQHS6O5QVV40QID
                     8QU0Q7
                     4"
                     /note="/building_history_54110=QUEEN.dna_dict['SI680_56']–=
                     –QUEEN 
                     (seq='AATTGGATCCTTACTTGTACAGCTCGTCCA',–product='SI680')"
                     /note="/building_history_54110="
                     /note="/building_history_54110=pLV_CS_659-6P8XHIXQXDGT13GN8
                     7MC059
                     W"
                     /note="/building_history_54152=QUEEN.dna_dict['SI1295_70']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCGTGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1295')"
                     /note="/building_history_54152="
                     /note="/building_history_54152=pLV_CS_659-5XBOFATD4UUW3F6OT
                     BESP0V
                     Q"
                     /note="/building_history_54154=QUEEN.dna_dict['SI1297_70']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCATGAGCAAGGGCGAGGAGGATAA
                      CGCTGCCATC',–product='SI1297')"
                     /note="/building_history_54154="
                     /note="/building_history_54154=pLV_CS_659-EQNQ0IQHB0WD8D3DS
                     FAYZ4D
                     R"
                     /note="/building_history_54155=QUEEN.dna_dict['SI1298_48']–
                     =–QUEE 
                     N(seq='GGTGAATTCCCGAGCGTGTCAGGGTGACCATGGCCATCATCAAGGAGTTCA'
                      ,–product='SI1298')"
                     /note="/building_history_54155="
                     /note="/building_history_54155=pLV_CS_659-1WPM3F1LMVUE40QID
                     8QU0Q7
                     4"
                     /note="/building_history_55552=QUEEN.dna_dict['dna_13128']–
                     =–QUEE N(seq='GAGGATAACATGGCCATC')"
                     /note="/building_history_55552="
                     /note="/building_history_55552=pLV_CS_659-32PHOG239CB6BDZNN
                     BNLPEG
                     8"
                     /note="/building_history_55562=QUEEN.dna_dict['dna_13130']–
                     =–QUEE N(seq='GAGGATAACATGGCCATC')"
                     /note="/building_history_55562="
                     /note="/building_history_55562=pLV_CS_659-CGVNJFLRV159BDZNN
                     BNLPEG
                     8"
                     /note="/building_history_55570=QUEEN.queried_features_dict[
                     'pLVSI 
                     N_CMV_pur_f15838']–=–QUEEN.dna_dict['pLVSIN_CMV_pur_12'].se
                      
                     archsequence(query=cs.lib['EcoRI'].cutsite,–product='re1sit
                      e')"
                     /note="/building_history_55570=query: 
                     cs.lib['EcoRI'].cutsite"
                     /note="/building_history_55570=pLV_CS_659-7EGNZ1V84QX06MJ83
                     Q2A4L5
                     D"
                     /note="/building_history_55571=QUEEN.queried_features_dict[
                     'pLVSI 
                     N_CMV_pur_f15839']–=–QUEEN.dna_dict['pLVSIN_CMV_pur_12'].se
                      
                     archsequence(query=cs.lib['BamHI'].cutsite,–product='re2sit
                      e')"
                     /note="/building_history_55571=query: 
                     cs.lib['BamHI'].cutsite"
                     /note="/building_history_55571=pLV_CS_659-4MBJFBQB33RP7I2DI
                     W16NRY
                     E"
                     /note="/building_history_55572=QUEEN.dna_dict['digested_bac
                     kbone_ 
                     436']–=–cropdna(QUEEN.dna_dict['pLVSIN_CMV_pur_12'],–start=
                      
                     QUEEN.queried_features_dict['pLVSIN_CMV_pur_f15839'][0],–en
                      
                     d=QUEEN.queried_features_dict['pLVSIN_CMV_pur_f15838'][0],–
                      
                     product='digested_backbone',–process_name='Digest–backbone'
                      
                     ,–process_description='Vector–backbone–plasmid–was–digested
                      
                     –with–two–distinct–restriction–enzymes–(see–Table–S2–for–de
                      
                     tails)–with–rCutSmart–Buffer–(NEB–#B6004S)–at–37ºC,–then–si
                      
                     ze-selected–and–purified–using–GeneJET–Gel–Extraction–Kit–(
                      Thermo–Fisher–Scientific–#K0691).')"
                     /note="/building_history_55572=start: '2916/2920'; end:
                     '2884/2888'"
                     /note="/building_history_55572=pLV_CS_659-30FOBQF7OXYO53K3M
                     T8476Z
                     R"
                     /note="/building_history_55578=QUEEN.dna_dict['dna_13132']–
                     =–QUEE N(seq='GAGGATAACATGGCCATC')"
                     /note="/building_history_55578="
                     /note="/building_history_55578=pLV_CS_659-3ZW93CNB2DJYBDZNN
                     BNLPEG
                     8"
                     /note="/building_history_55586=QUEEN.queried_features_dict[
                     'pLV_m 
                     Cherry_f15849']–=–QUEEN.dna_dict['pLV_mCherry_41'].searchse
                      
                     quence(query=QUEEN.dna_dict['SI680_56'].seq[-21:],–product=
                      'match')"
                     /note="/building_history_55586=query:
                     QUEEN.dna_dict['SI680_56'].seq[-21:]"
                     /note="/building_history_55586=pLV_CS_659-EMVUV5MC6XJRC97SU
                     DAAXZ5
                     1"
                     /note="/building_history_55588=QUEEN.dna_dict['dna_13134']–
                     =–QUEE N(seq='GAGGATAACATGGCCATC')"
                     /note="/building_history_55588="
                     /note="/building_history_55588=pLV_CS_659-34UC6GX6A8STBDZNN
                     BNLPEG
                     8"
                     /note="/building_history_55589=QUEEN.queried_features_dict[
                     'pLV_m 
                     Cherry_f15851']–=–QUEEN.dna_dict['pLV_mCherry_41'].searchse
                      quence(query=QUEEN.dna_dict['dna_13134'].seq)"
                     /note="/building_history_55589=query:
                     QUEEN.dna_dict['dna_13134'].seq"
                     /note="/building_history_55589=pLV_CS_659-733BCY0NFMS1F52IF
                     0KFUTF
                     H"
                     /note="/building_history_55590=QUEEN.dna_dict['extract1_665
                     ']–=–c 
                     ropdna(QUEEN.dna_dict['pLV_mCherry_41'],–start=QUEEN.querie
                      
                     d_features_dict['pLV_mCherry_f15851'][0].end,–end=QUEEN.que
                      
                     ried_features_dict['pLV_mCherry_f15849'][0].start,–product=
                      
                     'extract1',–process_name='Amplify–insert',–process_descript
                      
                     ion='Insert–fragments–were–amplified–by–Phusion–High-Fideli
                      
                     ty–DNA–Polymerase–(NEB–#M0530)–using–forward–and–reverse–pr
                      
                     imers–(see–Table–S2–for–details)–according–to–the–manufactu
                      rer's–PCR–protocol.')"
                     /note="/building_history_55590=start: 3766; end: 4421"
                     /note="/building_history_55590=pLV_CS_659-1BNN9AC43YI51Y41X
                     SJG120
                     M"
                     /note="/building_history_55592=QUEEN.dna_dict['PCR_product_
                     665']– 
                     =–modifyends(QUEEN.dna_dict['extract1_665'],–left=QUEEN.dna
                      
                     _dict['SI1297_70'].seq,–right=QUEEN.dna_dict['SI680_56'].rc
                      
                     seq,–product='PCR_product',–process_name='Amplify–insert',–
                      
                     process_description='Insert–fragments–were–amplified–by–Phu
                      
                     sion–High-Fidelity–DNA–Polymerase–(NEB–#M0530)–using–forwar
                      
                     d–and–reverse–primers–(see–Table–S2–for–details)–according–
                      to–the–manufacturer's–PCR–protocol.')"
                     /note="/building_history_55592=left: 
                     GGTGAATTCCCGAGCGTGTCAGGGTGACCATGAGCAAGGGCGAGGAGGATAACGCTGCC
                      ATC; right: TGGACGAGCTGTACAAGTAAGGATCCAATT; leftobj: 
                     QUEEN.dna_dict['SI1297_70'].seq; rightobj: 
                     QUEEN.dna_dict['SI680_56'].rcseq"
                     /note="/building_history_55592=pLV_CS_659-D10JV0TTRLE3DD8IZ
                     EQ3AH0
                     Y"
                     /note="/building_history_55596=QUEEN.queried_features_dict[
                     'PCR_p 
                     roduct_665_f15854']–=–QUEEN.dna_dict['PCR_product_665'].sea
                      
                     rchsequence(query=cs.lib['EcoRI'].cutsite,–product='re1site
                      ')"
                     /note="/building_history_55596=query: 
                     cs.lib['EcoRI'].cutsite"
                     /note="/building_history_55596=pLV_CS_659-BWSKQJQAVOQR6MJ83
                     Q2A4L5
                     D"
                     /note="/building_history_55597=QUEEN.queried_features_dict[
                     'PCR_p 
                     roduct_665_f15855']–=–QUEEN.dna_dict['PCR_product_665'].sea
                      
                     rchsequence(query=cs.lib['BamHI'].cutsite,–product='re2site
                      ')"
                     /note="/building_history_55597=query: 
                     cs.lib['BamHI'].cutsite"
                     /note="/building_history_55597=pLV_CS_659-964G91ULO9ID7I2DI
                     W16NRY
                     E"
                     /note="/building_history_55598=QUEEN.dna_dict['digested_ins
                     ert_29 
                     5']–=–cropdna(QUEEN.dna_dict['PCR_product_665'],–start=QUEE
                      
                     N.queried_features_dict['PCR_product_665_f15854'][0],–end=Q
                      
                     UEEN.queried_features_dict['PCR_product_665_f15855'][0],–pr
                      
                     oduct='digested_insert',–process_name='Digest–insert',–proc
                      
                     ess_description='Insert–PCR–products–were–digested–with–two
                      
                     –distinct–restriction–enzymes–(see–Table–S2–for–details)–wi
                      th–rCutSmart–Buffer–(NEB–#B6004S)–at–37ºC.')"
                     /note="/building_history_55598=start: '4/8'; end: 
                     '738/742'"
                     /note="/building_history_55598=pLV_CS_659-86K6FULHQZZX17K49
                     Z00EB5
                     Q"
                     /note="/building_history_55600=QUEEN.dna_dict['pLV_CS_659']
                     –=–joi 
                     ndna(*[QUEEN.dna_dict['digested_insert_295'],–QUEEN.dna_dic
                      
                     t['digested_backbone_436']],–topology='circular',–product='
                      
                     pLV_CS_196',–process_name='Ligate',–process_description='Di
                      
                     gested–insert–and–vector–backbone–fragments–were–mixed–with
                      
                     –T4–DNA–Ligase–(NEB–#M0202L)–and–10x–T4–DNA–Ligase–Buffer–(
                      
                     NEB#B0202S)–according–to–the–manufacturer's–ligation–protoc
                      ol.')"
                     /note="/building_history_55600=topology: circular"
                     /note="/building_history_55600=pLV_CS_659-7RD1JJZ5PHP342DB6
                     N2AAR4
                     5"
     misc_feature    447..635
                     /label=5' LTR
     misc_feature    686..823
                     /label=Psi
     misc_feature    790..1143
                     /label=GAG
     misc_feature    1297..1595
                     /label=PRE
     misc_feature    1596..2012
                     /label=ENV
     misc_feature    2027..2144
                     /label=CPPT
     promoter        2201..2808
                     /label=CMV promoter
     promoter        2201..2585
                     /label=CMV enhancer
     promoter        2586..2789
                     /label=CMV promoter
     misc_feature    2882..2888
                     /label=MCS
                     /note="/broken_feature=pLVSIN_CMV_pur_12:900:40:GTGAATTCCTC
                     GAGACT AGTTCTAGAGCGGCCGCGGATCC:2881..2921:1..7"
     gene            2943..3597
                     /label=mCherry
                     /note="/broken_feature=pLV_mCherry_41:1200:711:ATGGTGAGCAAG
                     GGCGAG 
                     GAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTC
                      
                     CGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCA
                      
                     CCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATC
                      
                     CTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCC
                      
                     CGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCG
                      
                     AGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC
                      
                     TACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAA
                      
                     GACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG
                      
                     GCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAG
                      
                     ACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAA
                      
                     GTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCG
                      
                     AGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAA:3730..4441:37.
                      .691"
     CDS             2943..3597
                     /label=Translation 3731-4441
                     /note="/broken_feature=pLV_mCherry_41:1300:711:ATGGTGAGCAAG
                     GGCGAG 
                     GAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTC
                      
                     CGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCA
                      
                     CCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATC
                      
                     CTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCC
                      
                     CGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCG
                      
                     AGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC
                      
                     TACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAA
                      
                     GACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG
                      
                     GCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAG
                      
                     ACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAA
                      
                     GTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCG
                      
                     AGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAA:3730..4441:37.
                      .691"
     misc_feature    complement(3077..3079)
                     /label=PAM
     misc_feature    complement(3080..3099)
                     /label=mCherry stop sg3
     misc_feature    3094..3095
                     /label=STOP
     misc_feature    complement(3178..3180)
                     /label=PAM
     misc_feature    complement(3181..3200)
                     /label=mCherry stop sg5
     misc_feature    complement(3182..3184)
                     /label=PAM
     misc_feature    complement(3185..3204)
                     /label=mCherry stop sg4
     misc_feature    3619..3623
                     /label=MCS
                     /note="/broken_feature=pLVSIN_CMV_pur_12:900:40:GTGAATTCCTC
                     GAGACT AGTTCTAGAGCGGCCGCGGATCC:2881..2921:36..40"
     promoter        3633..4132
                     /label=PGK promoter
     misc_feature    4152..4751
                     /label=PuroR
     CDS             4152..4154
                     /codon_start=1
                     /label=Translation 3450-3452
                     /translation="M"
     CDS             4749..4751
                     /codon_start=1
                     /label=Translation 4047-4049
                     /translation=""
     misc_feature    4763..5356
                     /label=WPRE
     misc_feature    5606..5794
                     /label=3' LTR
     primer_bind     complement(5918..5938)
                     /label=M13-rev
     misc_binding    complement(5944..5966)
                     /label=LacO
     promoter        complement(5970..6000)
                     /label=lac promoter
     rep_origin      complement(6306..6988)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7086..7745)
                     /label=AmpR
     misc_feature    8099..8219
                     /label=SV40-PA terminator
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgctgg gtctctctgg ttagaccaga tctgagcctg ggagcccact tggcagtaca
     2401 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aggcattatg
     2461 cccagtacat gaccttatgg gactttccta cttgatggcc cgcctggcat tatgcccagt
     2521 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2581 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     2641 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     2701 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     2761 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     2821 gccatccacg ctgttttgac ctccatagaa gacaccgact ctactagagg atctatttcc
     2881 ggtgaattcc cgagcgtgtc agggtgacca tgagcaaggg cgaggaggat aacgctgcca
     2941 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     3001 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3061 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     3121 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     3181 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3241 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3301 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     3361 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     3421 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     3481 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     3541 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3601 acgagctgta caagtaagga tccaattcta ccgggtaggg gaggcgcttt tcccaaggca
     3661 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     3721 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     3781 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     3841 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     3901 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     3961 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     4021 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     4081 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     4141 ttaacgccgc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4201 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     4261 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     4321 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     4381 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     4441 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     4501 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     4561 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     4621 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4681 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     4741 ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     4801 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4861 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4921 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4981 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5041 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5101 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5161 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5221 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5281 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5341 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     5401 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     5461 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     5521 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     5581 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5641 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5701 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5761 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5821 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     5881 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5941 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6001 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6061 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6121 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6721 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8041 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     8101 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8161 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8221 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     8281 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     8341 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
