LOCUS       Exported                8409 bp ds-DNA     circular UNK 17-JAN-2023
DEFINITION  Plasmid was generated by QUEEN v1.2.0 
            (https://github.com/yachielab/QUEEN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="."
                     /note="/building_history_39221=QUEEN.dna_dict['pLV_SI_34']–
                     =–QUEE 
                     N(record='131127',–dbtype='addgene',–product='pLV_SI_112')"
                     /note="/building_history_39221="
                     /note="/building_history_39221=pRS140_14-8EF26V8T4PDZDOUQMO
                     31WWIJ
                     "
                     /note="/building_history_39730=QUEEN.dna_dict['pRS138_12']–
                     =–QUEE 
                     N(record='https://benchling.com/s/seq-XzjSjLvLMm8Atn4CCZnz'
                      ,–dbtype='benchling',–product='pRS138')"
                     /note="/building_history_39730="
                     /note="/building_history_39730=pRS140_14-ARDMRX2J1GZEB9NFAM
                     RI7EH3
                     "
                     /note="/building_history_45597=QUEEN.dna_dict['SI681_43']–=
                     –QUEEN (seq='CTACTAGAGGATCTATTTCCGGTG',–product='SI681')"
                     /note="/building_history_45597="
                     /note="/building_history_45597=pRS140_14-7LRPENZ4BXERPZALGW
                     D55KU4
                     "
                     /note="/building_history_45697=QUEEN.dna_dict['RS243_44']–=
                     –QUEEN 
                     (seq='GGTAGAATTGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCATGG
                      TAATACTCTAGAGCTTCATGTGGTCGGGGTAG',–product='RS243')"
                     /note="/building_history_45697="
                     /note="/building_history_45697=pRS140_14-84F1SDP3G0KGELGQGH
                     BWPOM1
                     "
                     /note="/building_history_45698=QUEEN.dna_dict['RS244_44']–=
                     –QUEEN 
                     (seq='GTAATACTCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTTGTAC
                      AGCTCGTCCATGCC',–product='RS244')"
                     /note="/building_history_45698="
                     /note="/building_history_45698=pRS140_14-VJLTRPC7TWHGEM45PQ
                     H35SOF
                     "
                     /note="/building_history_48120=QUEEN.dna_dict['SI681_44']–=
                     –QUEEN (seq='CTACTAGAGGATCTATTTCCGGTG',–product='SI681')"
                     /note="/building_history_48120="
                     /note="/building_history_48120=pRS140_14-F1VODEAMA0FQPZALGW
                     D55KU4
                     "
                     /note="/building_history_48220=QUEEN.dna_dict['RS243_45']–=
                     –QUEEN 
                     (seq='GGTAGAATTGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCATGG
                      TAATACTCTAGAGCTTCATGTGGTCGGGGTAG',–product='RS243')"
                     /note="/building_history_48220="
                     /note="/building_history_48220=pRS140_14-ES1OL3WT1LBYELGQGH
                     BWPOM1
                     "
                     /note="/building_history_48221=QUEEN.dna_dict['RS244_45']–=
                     –QUEEN 
                     (seq='GTAATACTCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTTGTAC
                      AGCTCGTCCATGCC',–product='RS244')"
                     /note="/building_history_48221="
                     /note="/building_history_48221=pRS140_14-G7LTLBQNF7D3EM45PQ
                     H35SOF
                     "
                     /note="/building_history_50890=QUEEN.dna_dict['SI681_46']–=
                     –QUEEN (seq='CTACTAGAGGATCTATTTCCGGTG',–product='SI681')"
                     /note="/building_history_50890="
                     /note="/building_history_50890=pRS140_14-58YIND8SKQRQPZALGW
                     D55KU4
                     "
                     /note="/building_history_50990=QUEEN.dna_dict['RS243_47']–=
                     –QUEEN 
                     (seq='GGTAGAATTGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCATGG
                      TAATACTCTAGAGCTTCATGTGGTCGGGGTAG',–product='RS243')"
                     /note="/building_history_50990="
                     /note="/building_history_50990=pRS140_14-4X4D7Q986LKYELGQGH
                     BWPOM1
                     "
                     /note="/building_history_50991=QUEEN.dna_dict['RS244_47']–=
                     –QUEEN 
                     (seq='GTAATACTCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTTGTAC
                      AGCTCGTCCATGCC',–product='RS244')"
                     /note="/building_history_50991="
                     /note="/building_history_50991=pRS140_14-84XEQQQQFRM2EM45PQ
                     H35SOF
                     "
                     /note="/building_history_54111=QUEEN.dna_dict['SI681_47']–=
                     –QUEEN (seq='CTACTAGAGGATCTATTTCCGGTG',–product='SI681')"
                     /note="/building_history_54111="
                     /note="/building_history_54111=pRS140_14-5N7KOA36F869PZALGW
                     D55KU4
                     "
                     /note="/building_history_54211=QUEEN.dna_dict['RS243_48']–=
                     –QUEEN 
                     (seq='GGTAGAATTGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCATGG
                      TAATACTCTAGAGCTTCATGTGGTCGGGGTAG',–product='RS243')"
                     /note="/building_history_54211="
                     /note="/building_history_54211=pRS140_14-ENR0X171FHUWELGQGH
                     BWPOM1
                     "
                     /note="/building_history_54212=QUEEN.dna_dict['RS244_48']–=
                     –QUEEN 
                     (seq='GTAATACTCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTTGTAC
                      AGCTCGTCCATGCC',–product='RS244')"
                     /note="/building_history_54212="
                     /note="/building_history_54212=pRS140_14-3YABRXPBSIVJEM45PQ
                     H35SOF
                     "
                     /note="/building_history_55231=QUEEN.queried_features_dict[
                     'pRS13 
                     8_f15582']–=–QUEEN.dna_dict['pRS138_12'].searchsequence(que
                      ry=cs.lib['EcoRI'].cutsite,–product='re1site')"
                     /note="/building_history_55231=query: 
                     cs.lib['EcoRI'].cutsite"
                     /note="/building_history_55231=pRS140_14-802X0ETDT5KO6MJ83Q
                     2A4L5D
                     "
                     /note="/building_history_55232=QUEEN.queried_features_dict[
                     'pRS13 
                     8_f15583']–=–QUEEN.dna_dict['pRS138_12'].searchsequence(que
                      ry=cs.lib['XbaI'].cutsite,–product='re2site')"
                     /note="/building_history_55232=query: 
                     cs.lib['XbaI'].cutsite"
                     /note="/building_history_55232=pRS140_14-523TDIVLH8PX2QR87M
                     9W3T19
                     "
                     /note="/building_history_55233=QUEEN.dna_dict['digested_bac
                     kbone_ 
                     426']–=–cropdna(QUEEN.dna_dict['pRS138_12'],–start=QUEEN.qu
                      
                     eried_features_dict['pRS138_f15583'][0],–end=QUEEN.queried_
                      
                     features_dict['pRS138_f15582'][0],–product='digested_backbo
                      
                     ne',–process_name='Digest–backbone',–process_description='V
                      
                     ector–backbone–plasmid–was–digested–with–two–distinct–restr
                      
                     iction–enzymes–(see–Table–S2–for–details)–with–rCutSmart–Bu
                      
                     ffer–(NEB–#B6004S)–at–37ºC,–then–size-selected–and–purified
                      
                     –using–GeneJET–Gel–Extraction–Kit–(Thermo–Fisher–Scientific
                      –#K0691).')"
                     /note="/building_history_55233=start: '3638/3642'; end:
                     '3371/3375'"
                     /note="/building_history_55233=pRS140_14-631EV8T33JNX53K3MT
                     8476ZR
                     "
                     /note="/building_history_55327=QUEEN.queried_features_dict[
                     'pLV_S 
                     I_112_f15653']–=–QUEEN.dna_dict['pLV_SI_34'].searchsequence
                      
                     (query=QUEEN.dna_dict['SI681_47'].seq[-24:],–product='match
                      ')"
                     /note="/building_history_55327=query:
                     QUEEN.dna_dict['SI681_47'].seq[-24:]"
                     /note="/building_history_55327=pRS140_14-2J4V24MUX6TD5IJ5G3
                     XN8AC3
                     "
                     /note="/building_history_55365=QUEEN.queried_features_dict[
                     'pLV_S 
                     I_112_f15691']–=–QUEEN.dna_dict['pLV_SI_34'].searchsequence
                      
                     (query=QUEEN.dna_dict['RS244_48'].seq[-55:],–product='match
                      ')"
                     /note="/building_history_55365=query:
                     QUEEN.dna_dict['RS244_48'].seq[-55:]"
                     /note="/building_history_55365=pRS140_14-46X8XUP1SZOH1LCJ1D
                     35IFAN
                     "
                     /note="/building_history_55367=QUEEN.dna_dict['extract1_655
                     ']–=–c 
                     ropdna(QUEEN.dna_dict['pLV_SI_34'],–start=QUEEN.queried_fea
                      
                     tures_dict['pLV_SI_112_f15653'][0].end,–end=QUEEN.queried_f
                      
                     eatures_dict['pLV_SI_112_f15691'][0].start,–product='extrac
                      
                     t1',–process_name='Amplify–insert',–process_description='In
                      
                     sert–fragments–were–amplified–by–Phusion–High-Fidelity–DNA–
                      
                     Polymerase–(NEB–#M0530)–using–forward–and–reverse–primers–(
                      
                     see–Table–S2–for–details)–according–to–the–manufacturer's–P
                      CR–protocol.')"
                     /note="/building_history_55367=start: 819; end: 1540"
                     /note="/building_history_55367=pRS140_14-9I971VHBS9HQ1Y41XS
                     JG120M
                     "
                     /note="/building_history_55369=QUEEN.dna_dict['PCR_product_
                     655']– 
                     =–modifyends(QUEEN.dna_dict['extract1_655'],–left=QUEEN.dna
                      
                     _dict['SI681_47'].seq,–right=QUEEN.dna_dict['RS244_48'].rcs
                      
                     eq,–product='PCR_product',–process_name='Amplify–insert',–p
                      
                     rocess_description='Insert–fragments–were–amplified–by–Phus
                      
                     ion–High-Fidelity–DNA–Polymerase–(NEB–#M0530)–using–forward
                      
                     –and–reverse–primers–(see–Table–S2–for–details)–according–t
                      o–the–manufacturer's–PCR–protocol.')"
                     /note="/building_history_55369=left: 
                     CTACTAGAGGATCTATTTCCGGTG;
                     right: 
                     GGCATGGACGAGCTGTACAAGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTAG
                      AGTATTAC; leftobj: QUEEN.dna_dict['SI681_47'].seq; 
                     rightobj: QUEEN.dna_dict['RS244_48'].rcseq"
                     /note="/building_history_55369=pRS140_14-7PQ6UY74Y8YMDD8IZE
                     Q3AH0Y
                     "
                     /note="/building_history_55507=QUEEN.queried_features_dict[
                     'PCR_p 
                     roduct_655_f15806']–=–QUEEN.dna_dict['PCR_product_655'].sea
                      
                     rchsequence(query=cs.lib['EcoRI'].cutsite,–product='re1site
                      ')"
                     /note="/building_history_55507=query: 
                     cs.lib['EcoRI'].cutsite"
                     /note="/building_history_55507=pRS140_14-1QOKGVLDF1JA6MJ83Q
                     2A4L5D
                     "
                     /note="/building_history_55508=QUEEN.queried_features_dict[
                     'PCR_p 
                     roduct_655_f15807']–=–QUEEN.dna_dict['PCR_product_655'].sea
                      
                     rchsequence(query=cs.lib['XbaI'].cutsite,–product='re2site'
                      )"
                     /note="/building_history_55508=query: 
                     cs.lib['XbaI'].cutsite"
                     /note="/building_history_55508=pRS140_14-1R5Y2JOXCDRY2QR87M
                     9W3T19
                     "
                     /note="/building_history_55509=QUEEN.dna_dict['digested_ins
                     ert_28 
                     7']–=–cropdna(QUEEN.dna_dict['PCR_product_655'],–start=QUEE
                      
                     N.queried_features_dict['PCR_product_655_f15806'][0],–end=Q
                      
                     UEEN.queried_features_dict['PCR_product_655_f15807'][0],–pr
                      
                     oduct='digested_insert',–process_name='Digest–insert',–proc
                      
                     ess_description='Insert–PCR–products–were–digested–with–two
                      
                     –distinct–restriction–enzymes–(see–Table–S2–for–details)–wi
                      th–rCutSmart–Buffer–(NEB–#B6004S)–at–37ºC.')"
                     /note="/building_history_55509=start: '24/28'; end: 
                     '800/804'"
                     /note="/building_history_55509=pRS140_14-BJMUA4WM243L17K49Z
                     00EB5Q
                     "
                     /note="/building_history_55534=QUEEN.dna_dict['pRS140_14']–
                     =–join 
                     dna(*[QUEEN.dna_dict['digested_insert_287'],–QUEEN.dna_dict
                      
                     ['digested_backbone_426']],–topology='circular',–product='p
                      
                     RS140',–process_name='Ligate',–process_description='Digeste
                      
                     d–insert–and–vector–backbone–fragments–were–mixed–with–T4–D
                      
                     NA–Ligase–(NEB–#M0202L)–and–10x–T4–DNA–Ligase–Buffer–(NEB#B
                      
                     0202S)–according–to–the–manufacturer's–ligation–protocol.')
                      "
                     /note="/building_history_55534=topology: circular"
                     /note="/building_history_55534=pRS140_14-9N4CW3WQ7KCN8J4AYP
                     AWI32M
                     "
     source          3372..4092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="/broken_feature=pLV_SI_34:0:8328:-:0..8328:820..1540
                     "
     misc_feature    269..389
                     /label=SV40-PA terminator
     misc_feature    1015..1203
                     /label=5' LTR
     misc_feature    1254..1391
                     /label=Psi
     misc_feature    1358..1711
                     /label=GAG
     misc_feature    1865..2163
                     /label=PRE
     misc_feature    2164..2580
                     /label=ENV
     misc_feature    2595..2712
                     /label=CPPT
     promoter        2760..3344
                     /label=CMV promoter
     promoter        2769..3072
                     /label=CMV enhancer
     promoter        3073..3276
                     /label=CMV promoter
     misc_feature    3348..3454
                     /label=1st PCR, MiSeq amplicon
     misc_feature    3371..3376
                     /label=EcoR1
     CDS             3400..4116
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             3400..4113
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3442..3463)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3703..3722)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4050..4071
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     gap             4117..4146
                     /estimated_length=30
     misc_feature    4153..4166
                     /label=pcr binding site
     misc_feature    4167..4196
                     /label=polyA30
     misc_feature    4197..4214
                     /label=primer binding
                     
     misc_feature    4197
                     /label=Spacer
     misc_feature    4198..4202
                     /label=MCS
     promoter        4212..4711
                     /label=PGK promoter
     misc_feature    4731..5330
                     /label=PuroR
     misc_feature    5342..5935
                     /label=WPRE
     misc_feature    6185..6373
                     /label=3' LTR
     primer_bind     complement(6497..6517)
                     /label=M13-rev
     misc_binding    complement(6523..6545)
                     /label=LacO
     promoter        complement(6549..6579)
                     /label=lac promoter
     rep_origin      complement(6885..7567)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7665..8324)
                     /label=AmpR
ORIGIN
        1 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
       61 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgacgtcg acggatcggg agatcaactt tgtttattgc agcttataat ggttacaaat
      301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      361 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
      421 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
      481 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
      541 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
      601 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
      661 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
      721 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
      781 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
      841 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
      901 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
      961 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     1021 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     1081 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     1141 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     1201 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     1261 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     1321 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     1381 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     1441 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1501 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1561 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     1621 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1681 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     1741 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1801 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1861 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1921 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1981 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     2041 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     2101 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     2161 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     2221 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     2281 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     2341 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     2401 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     2461 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     2521 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     2581 ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     2641 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2701 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg ataagcttgg
     2761 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2821 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2881 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2941 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3001 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3061 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     3121 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     3181 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3241 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3301 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta ctagaggatc
     3361 tatttccggt gaattcccga gcgtgtcagg gtgaccgtgg tgagcaaggg cgaggagctg
     3421 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3481 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3541 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3601 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3661 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3721 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3781 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3841 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3901 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3961 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4021 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4081 gggatcactc tcggcatgga cgagctgtac aagtaannnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnntcta gagtattacc atggtcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaggat
     4201 ccaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     4261 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4321 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4381 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4441 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4501 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     4561 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     4621 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     4681 gttctcctct tcctcatctc cgggcctttc gacctgcagt taacgccgcc atgaccgagt
     4741 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     4801 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     4861 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4921 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4981 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5041 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5101 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5161 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5221 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5281 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg
     5341 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5401 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5461 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5521 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5581 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5641 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5701 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5761 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5821 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5881 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     5941 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6001 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6061 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6121 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6181 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6241 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6301 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6361 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6421 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc
     6481 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8401 cgtgcaccc
//
