LOCUS       Exported                8498 bp ds-DNA     circular UNK 17-JAN-2023
DEFINITION  Plasmid was generated by QUEEN v1.2.0 
            (https://github.com/yachielab/QUEEN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="."
                     /note="/building_history_39738=QUEEN.dna_dict['pRS176_1']–=
                     –QUEEN 
                     (record='https://benchling.com/s/seq-3zhUL2NJVBnoY0ZtbfZX',
                      –dbtype='benchling',–product='pRS176')"
                     /note="/building_history_39738="
                     /note="/building_history_39738=pRS193_15-16PTLX99CVCE6RN5OY
                     JVDBUS
                     "
                     /note="/building_history_39739=QUEEN.dna_dict['pRS178_1']–=
                     –QUEEN 
                     (record='https://benchling.com/s/seq-82d6VWOf9T7NvkbiD8o9',
                      –dbtype='benchling',–product='pRS178')"
                     /note="/building_history_39739="
                     /note="/building_history_39739=pRS193_15-73IMJX2SE6XE3J1FYA
                     7MUCM9
                     "
                     /note="/building_history_48110=QUEEN.dna_dict['SI447_32']–=
                     –QUEEN (seq='GCCACATAGCAGAACTTTAAAAGTG',–product='SI447')"
                     /note="/building_history_48110="
                     /note="/building_history_48110=pRS193_15-56AMITBBLOBQCPKD9C
                     4M9FBE
                     "
                     /note="/building_history_48223=QUEEN.dna_dict['RS307_31']–=
                     –QUEEN 
                     (seq='CAGGTGTCGTGAACTCTACTAGAGGATCTATTTCC',–product='RS307'
                      )"
                     /note="/building_history_48223="
                     /note="/building_history_48223=pRS193_15-CKK86S3FC794C10N6M
                     9GXOK4
                     "
                     /note="/building_history_49633=QUEEN.dna_dict['SI447_33']–=
                     –QUEEN (seq='GCCACATAGCAGAACTTTAAAAGTG',–product='SI447')"
                     /note="/building_history_49633="
                     /note="/building_history_49633=pRS193_15-8Z4LQL7NDW0TCPKD9C
                     4M9FBE
                     "
                     /note="/building_history_49634=QUEEN.dna_dict['SI448_32']–=
                     –QUEEN (seq='CACTTTTAAAGTTCTGCTATGTGGC',–product='SI448')"
                     /note="/building_history_49634="
                     /note="/building_history_49634=pRS193_15-53AORE219LSXCHRWJD
                     42CJRO
                     "
                     /note="/building_history_49746=QUEEN.dna_dict['RS307_32']–=
                     –QUEEN 
                     (seq='CAGGTGTCGTGAACTCTACTAGAGGATCTATTTCC',–product='RS307'
                      )"
                     /note="/building_history_49746="
                     /note="/building_history_49746=pRS193_15-8OGBWD88BSAJC10N6M
                     9GXOK4
                     "
                     /note="/building_history_49747=QUEEN.dna_dict['RS308_31']–=
                     –QUEEN 
                     (seq='CCTCTAGTAGAGTTCACGACACCTGAAATGGAA',–product='RS308')"
                     /note="/building_history_49747="
                     /note="/building_history_49747=pRS193_15-E1RPB30IRI5K51S0KQ
                     WZLD3D
                     "
                     /note="/building_history_50880=QUEEN.dna_dict['SI447_34']–=
                     –QUEEN (seq='GCCACATAGCAGAACTTTAAAAGTG',–product='SI447')"
                     /note="/building_history_50880="
                     /note="/building_history_50880=pRS193_15-BXGP9Z69IIRJCPKD9C
                     4M9FBE
                     "
                     /note="/building_history_50881=QUEEN.dna_dict['SI448_33']–=
                     –QUEEN (seq='CACTTTTAAAGTTCTGCTATGTGGC',–product='SI448')"
                     /note="/building_history_50881="
                     /note="/building_history_50881=pRS193_15-169MPZ8WT8MTCHRWJD
                     42CJRO
                     "
                     /note="/building_history_50993=QUEEN.dna_dict['RS307_33']–=
                     –QUEEN 
                     (seq='CAGGTGTCGTGAACTCTACTAGAGGATCTATTTCC',–product='RS307'
                      )"
                     /note="/building_history_50993="
                     /note="/building_history_50993=pRS193_15-EO6GDI31F90QC10N6M
                     9GXOK4
                     "
                     /note="/building_history_50994=QUEEN.dna_dict['RS308_32']–=
                     –QUEEN 
                     (seq='CCTCTAGTAGAGTTCACGACACCTGAAATGGAA',–product='RS308')"
                     /note="/building_history_50994="
                     /note="/building_history_50994=pRS193_15-E8RUIW7BSQKY51S0KQ
                     WZLD3D
                     "
                     /note="/building_history_54101=QUEEN.dna_dict['SI447_35']–=
                     –QUEEN (seq='GCCACATAGCAGAACTTTAAAAGTG',–product='SI447')"
                     /note="/building_history_54101="
                     /note="/building_history_54101=pRS193_15-75864JZ8JP69CPKD9C
                     4M9FBE
                     "
                     /note="/building_history_54102=QUEEN.dna_dict['SI448_34']–=
                     –QUEEN (seq='CACTTTTAAAGTTCTGCTATGTGGC',–product='SI448')"
                     /note="/building_history_54102="
                     /note="/building_history_54102=pRS193_15-D73CPKRU64PACHRWJD
                     42CJRO
                     "
                     /note="/building_history_54214=QUEEN.dna_dict['RS307_34']–=
                     –QUEEN 
                     (seq='CAGGTGTCGTGAACTCTACTAGAGGATCTATTTCC',–product='RS307'
                      )"
                     /note="/building_history_54214="
                     /note="/building_history_54214=pRS193_15-60WG43Y670M0C10N6M
                     9GXOK4
                     "
                     /note="/building_history_54215=QUEEN.dna_dict['RS308_33']–=
                     –QUEEN 
                     (seq='CCTCTAGTAGAGTTCACGACACCTGAAATGGAA',–product='RS308')"
                     /note="/building_history_54215="
                     /note="/building_history_54215=pRS193_15-70QZ0HIWAQ3U51S0KQ
                     WZLD3D
                     "
                     /note="/building_history_58196=QUEEN.dna_dict['SI447_36']–=
                     –QUEEN (seq='GCCACATAGCAGAACTTTAAAAGTG',–product='SI447')"
                     /note="/building_history_58196="
                     /note="/building_history_58196=pRS193_15-C3ZM4MQ4RZFACPKD9C
                     4M9FBE
                     "
                     /note="/building_history_58197=QUEEN.dna_dict['SI448_35']–=
                     –QUEEN (seq='CACTTTTAAAGTTCTGCTATGTGGC',–product='SI448')"
                     /note="/building_history_58197="
                     /note="/building_history_58197=pRS193_15-5V2CCVXO5J87CHRWJD
                     42CJRO
                     "
                     /note="/building_history_58309=QUEEN.dna_dict['RS307_35']–=
                     –QUEEN 
                     (seq='CAGGTGTCGTGAACTCTACTAGAGGATCTATTTCC',–product='RS307'
                      )"
                     /note="/building_history_58309="
                     /note="/building_history_58309=pRS193_15-72I7N5SX29JDC10N6M
                     9GXOK4
                     "
                     /note="/building_history_58310=QUEEN.dna_dict['RS308_34']–=
                     –QUEEN 
                     (seq='CCTCTAGTAGAGTTCACGACACCTGAAATGGAA',–product='RS308')"
                     /note="/building_history_58310="
                     /note="/building_history_58310=pRS193_15-D2NL2JAOKSLQ51S0KQ
                     WZLD3D
                     "
                     /note="/building_history_59047=QUEEN.queried_features_dict[
                     'pRS17 
                     8_f16699']–=–QUEEN.dna_dict['pRS178_1'].searchsequence(quer
                      y=QUEEN.dna_dict['SI447_36'].seq[-25:],–product='match')"
                     /note="/building_history_59047=query:
                     QUEEN.dna_dict['SI447_36'].seq[-25:]; _source: tmpsyxktm3s"
                     /note="/building_history_59047=pRS193_15-EQMEA4GHY3LV4BDJCL
                     C3W94Y
                     "
                     /note="/building_history_59050=QUEEN.queried_features_dict[
                     'pRS17 
                     8_f16702']–=–QUEEN.dna_dict['pRS178_1'].searchsequence(quer
                      y=QUEEN.dna_dict['RS307_35'].seq[-20:],–product='match')"
                     /note="/building_history_59050=query:
                     QUEEN.dna_dict['RS307_35'].seq[-20:]; _source: tmpsyxktm3s"
                     /note="/building_history_59050=pRS193_15-ECT1C5RGJXGTF0NRCW
                     ASAAD3
                     "
                     /note="/building_history_59052=QUEEN.dna_dict['extract1_692
                     ']–=–c 
                     ropdna(QUEEN.dna_dict['pRS178_1'],–start=QUEEN.queried_feat
                      
                     ures_dict['pRS178_f16699'][0].end,–end=QUEEN.queried_featur
                      
                     es_dict['pRS178_f16702'][0].start,–product='extract1',–proc
                      
                     ess_name='Amplify–backbone',–process_description='Backbone–
                      
                     fragments–were–amplified–by–Phusion–High-Fidelity–DNA–Polym
                      
                     erase–(NEB–#M0530)–using–forward–and–reverse–primers–and–1–
                      
                     ng–of–template–plasmid–(see–Table–S2–for–details)–according
                      –to–the–manufacturer's–PCR–protocol.')"
                     /note="/building_history_59052=start: 8416; end: 3090; 
                     _source:
                     tmpsyxktm3s"
                     /note="/building_history_59052=pRS193_15-6BVBUE1K57OY5XSV9O
                     3T4JOC
                     "
                     /note="/building_history_59054=QUEEN.dna_dict['PCR_product_
                     693']– 
                     =–modifyends(QUEEN.dna_dict['extract1_692'],–left=QUEEN.dna
                      
                     _dict['SI447_36'].seq,–right=QUEEN.dna_dict['RS307_35'].rcs
                      
                     eq,–product='PCR_product',–process_name='Amplify–backbone',
                      
                     –process_description='Backbone–fragments–were–amplified–by–
                      
                     Phusion–High-Fidelity–DNA–Polymerase–(NEB–#M0530)–using–for
                      
                     ward–and–reverse–primers–and–1–ng–of–template–plasmid–(see–
                      
                     Table–S2–for–details)–according–to–the–manufacturer's–PCR–p
                      rotocol.')"
                     /note="/building_history_59054=left: 
                     GCCACATAGCAGAACTTTAAAAGTG;
                     right: GGAAATAGATCCTCTAGTAGAGTTCACGACACCTG; leftobj: 
                     QUEEN.dna_dict['SI447_36'].seq; rightobj: 
                     QUEEN.dna_dict['RS307_35'].rcseq; _source: tmpsyxktm3s"
                     /note="/building_history_59054=pRS193_15-80C8WKU3UITK255P7Y
                     BUXNA4
                     "
                     /note="/building_history_59433=QUEEN.queried_features_dict[
                     'pRS17 
                     6_f17043']–=–QUEEN.dna_dict['pRS176_1'].searchsequence(quer
                      y=QUEEN.dna_dict['RS308_34'].seq[-20:],–product='match')"
                     /note="/building_history_59433=query:
                     QUEEN.dna_dict['RS308_34'].seq[-20:]; _source: tmpsyxktm3s"
                     /note="/building_history_59433=pRS193_15-1RBCHAUQ0L9IF0NRCW
                     ASAAD3
                     "
                     /note="/building_history_59442=QUEEN.queried_features_dict[
                     'pRS17 
                     6_f17052']–=–QUEEN.dna_dict['pRS176_1'].searchsequence(quer
                      y=QUEEN.dna_dict['SI448_35'].seq[-25:],–product='match')"
                     /note="/building_history_59442=query:
                     QUEEN.dna_dict['SI448_35'].seq[-25:]; _source: tmpsyxktm3s"
                     /note="/building_history_59442=pRS193_15-49OUCGDM1CKI4BDJCL
                     C3W94Y
                     "
                     /note="/building_history_59443=QUEEN.dna_dict['extract1_706
                     ']–=–c 
                     ropdna(QUEEN.dna_dict['pRS176_1'],–start=QUEEN.queried_feat
                      
                     ures_dict['pRS176_f17043'][0].end,–end=QUEEN.queried_featur
                      
                     es_dict['pRS176_f17052'][0].start,–product='extract1',–proc
                      
                     ess_name='Amplify–insert',–process_description='Insert–frag
                      
                     ments–were–amplified–by–Phusion–High-Fidelity–DNA–Polymeras
                      
                     e–(NEB–#M0530)–using–forward–and–reverse–primers–and–1–ng–o
                      
                     f–template–plasmid–(see–Table–S2–for–details)–according–to–
                      the–manufacturer's–PCR–protocol.')"
                     /note="/building_history_59443=start: 3650; end: 8900; 
                     _source:
                     tmpsyxktm3s"
                     /note="/building_history_59443=pRS193_15-39V2CXHO6IK4AC6FXC
                     CT6M0N
                     "
                     /note="/building_history_59445=QUEEN.dna_dict['PCR_product_
                     707']– 
                     =–modifyends(QUEEN.dna_dict['extract1_706'],–left=QUEEN.dna
                      
                     _dict['RS308_34'].seq,–right=QUEEN.dna_dict['SI448_35'].rcs
                      
                     eq,–product='PCR_product',–process_name='Amplify–insert',–p
                      
                     rocess_description='Insert–fragments–were–amplified–by–Phus
                      
                     ion–High-Fidelity–DNA–Polymerase–(NEB–#M0530)–using–forward
                      
                     –and–reverse–primers–and–1–ng–of–template–plasmid–(see–Tabl
                      
                     e–S2–for–details)–according–to–the–manufacturer's–PCR–proto
                      col.')"
                     /note="/building_history_59445=left:
                     CCTCTAGTAGAGTTCACGACACCTGAAATGGAA; right: 
                     GCCACATAGCAGAACTTTAAAAGTG; leftobj: 
                     QUEEN.dna_dict['RS308_34'].seq; rightobj: 
                     QUEEN.dna_dict['SI448_35'].rcseq; _source: tmpsyxktm3s"
                     /note="/building_history_59445=pRS193_15-BO7W0E4FCAWN71GXXS
                     5VJBTL
                     "
                     /note="/building_history_59682=QUEEN.dna_dict['PCR_product_
                     723']– 
                     =–cropdna(QUEEN.dna_dict['PCR_product_707'],–start='25/25',
                      –end='5283/5283')"
                     /note="/building_history_59682=start: 25; end: 5283; 
                     _source:
                     tmpsyxktm3s"
                     /note="/building_history_59682=pRS193_15-4E8M48ZAEFRE14DYT4
                     4LY8TS
                     "
                     /note="/building_history_59683=QUEEN.dna_dict['pRS193_15']–
                     =–join 
                     dna(*[QUEEN.dna_dict['PCR_product_723'],–QUEEN.dna_dict['PC
                      
                     R_product_693']],–topology='circular',–product='pRS193',–pr
                      
                     ocess_name='Assemble',–process_description='Higher–molar–ra
                      
                     tios–of–insert–to–digested–backbone–fragments–were–mixed–to
                      
                     –a–total–volume–of–20–µL–with–2–µL–of–5x–ISO–Buffer,–0.2–µL
                      
                     –of–T5–Exonuclease–(NEB–#M0363L),–1–µL–of–Taq–DNA–Ligase–(N
                      
                     EB–#M0208S),–and–0.125–µL–of–Phusion–High-Fidelity–DNA–Poly
                      merase–(NEB–#M0530)–and–incubated–at–50ºC–for–1–hour.')"
                     /note="/building_history_59683=topology: circular; _source:
                     tmpsyxktm3s"
                     /note="/building_history_59683=pRS193_15-CONUG7BSMKJH2IGF9H
                     5PINBI
                     "
     promoter        complement(1..1152)
                     /label=EF1-alpha promoter
                     /note="/broken_feature=pRS176_1:2100:1172:GTGCCCGTCAGTGGGCA
                     GAGCGC 
                     ACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGATCCGGTGCCTA
                      
                     GAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTC
                      
                     CCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGC
                      
                     AACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCT
                      
                     CTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGA
                      
                     TTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAA
                      
                     GGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGT
                      
                     GCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTT
                      
                     AAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCG
                      
                     GGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCG
                      
                     TGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCG
                      
                     GACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGT
                      
                     ATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAG
                      
                     ATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAG
                      
                     AGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCT
                      
                     TCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTT
                      
                     TTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACA
                      
                     CTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAA
                      
                     TTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAG
                      TTTTTTTCTTCCATTTCAGGTGTCGTGA:3630..4802:1152..1"
     misc_feature    1153
                     /label=Spacer
     misc_feature    1154..1158
                     /label=MCS
     promoter        1168..1667
                     /label=PGK promoter
     misc_feature    1687..2286
                     /label=PuroR
     misc_feature    2298..2891
                     /label=WPRE
     misc_feature    3141..3329
                     /label=3' LTR
     primer_bind     complement(3453..3473)
                     /label=M13-rev
     misc_binding    complement(3479..3501)
                     /label=LacO
     promoter        complement(3505..3535)
                     /label=lac promoter
     rep_origin      complement(3841..4523)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(4621..5280)
                     /label=AmpR
     misc_feature    5634..5754
                     /label=SV40-PA terminator
     misc_feature    6380..6568
                     /label=5' LTR
     misc_feature    6619..6756
                     /label=Psi
     misc_feature    6723..7076
                     /label=GAG
     misc_feature    7230..7528
                     /label=PRE
     misc_feature    7529..7945
                     /label=ENV
     misc_feature    7960..8077
                     /label=CPPT
     misc_feature    8118..8475
                     /label=insert
     promoter        complement(8118..8120)
                     /label=PGK promoter
     misc_feature    8130..8134
                     /label=MCS
     misc_feature    8135
                     /label=Spacer
     misc_feature    complement(8136..8165)
                     /label=polyA30
     misc_feature    complement(8166..8179)
                     /label=pcr binding site
     gene            complement(8186..8423)
                     /label=trucEGFP (Non-ATG)
     CDS             complement(8186..8423)
                     /codon_start=1
                     /label=Translation 3058-2821
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMK"
     misc_feature    8369..8475
                     /label=1st PCR, MiSeq amplicon
     misc_feature    8424..8426
                     /label=GTG
     misc_feature    8426
                     /label=C>T
     misc_feature    8427..8443
                     /label=112-V4-BC2
     misc_feature    8444..8446
                     /label=PAM
     misc_feature    complement(8447..8452)
                     /label=EcoR1
ORIGIN
        1 gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa
       61 gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc
      121 actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac
      181 tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac
      241 atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc
      301 gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc
      361 atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga
      421 tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc
      481 cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac
      541 gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg
      601 catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa
      661 tggctagaga cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc
      721 ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag
      781 cgcaaggcct cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt
      841 actgcagcca ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag
      901 gcccgcggga accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa
      961 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     1021 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     1081 atcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     1141 actgacgggc acggatccaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     1201 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     1261 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     1321 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     1381 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     1441 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     1501 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     1561 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     1621 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagttaac
     1681 gccgccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     1741 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     1801 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     1861 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     1921 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     1981 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2041 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2101 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2161 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2221 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2281 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2341 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2401 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2461 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2521 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2581 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2641 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     2701 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2761 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2821 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2881 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     2941 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     3001 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     3061 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     3121 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3301 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     3361 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     3421 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4021 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4261 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4501 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4561 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4621 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4681 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4741 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4801 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4861 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4921 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4981 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5041 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5101 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5161 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5221 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5281 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5341 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5401 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5461 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5521 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5581 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aactttgttt
     5641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5761 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     5821 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     5881 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
     5941 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
     6001 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     6061 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     6121 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     6181 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     6241 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     6301 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     6361 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6421 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6481 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6541 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6601 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     6661 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     6721 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     6781 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     6841 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     6901 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     6961 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7021 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7081 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     7141 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     7201 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     7261 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     7321 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     7381 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     7441 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     7501 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     7561 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     7621 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     7681 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     7741 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     7801 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     7861 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     7921 agatccattc gattagtgaa cggatctcga cggtatcgcc tttaaaagaa aaggggggat
     7981 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8041 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     8101 agatccagtt tatcgatccc ggtagaattg gatccttttt tttttttttt tttttttttt
     8161 tttttgacca tggtaatact ctagagcttc atgtggtcgg ggtagcggct gaagcactgc
     8221 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     8281 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     8341 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     8401 aacagctcct cgcccttgct caccacggtc accctgacac gctcgggaat tcaccggaaa
     8461 tagatcctct agtagagttc acgacacctg aaatggaa
//
