LOCUS       Exported                3395 bp ds-DNA     linear   UNA 04-MAR-2022
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        495..772
                     /label=chicken beta-actin promoter
     regulatory      1193..1198
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1199..1597
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
     CDS             1622..1678
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1691..2407
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    2432..3020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2903..2914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3063..3184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cagtaccatg acgtcaggcg gccatcgtgg tacgccgatc cgtgccggtc gctgattatt
       61 atgcagctgc ccgctcgctc gctcactgag ccgccgggca aagcccgggc gtcgggcgac
      121 tttgttcgcc cgccctcagt gaccgaccga accgccccag agaggagtgc actcatcact
      181 agggtctgta gtaatgatta acccgccatg ctactatcta cgtagccatg ctctaggaag
      241 agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      481 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
      541 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
      601 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
      661 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
      721 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      781 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      841 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      901 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      961 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg
     1021 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1081 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1141 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccggta ccgccaccat
     1201 ggctaagact tccgaacaga gggtgaacat tgctacactg ctgacagaaa ataagaagaa
     1261 aatcgtggat aaggcttccc aggatctgtg gcggagacac ccagacctga tcgcaccagg
     1321 aggaattgct ttctctcaga gggaccgcgc tctgtgcctg cgagattacg gctggttcct
     1381 gcatctgatc accttttgtc tgctggccgg agataagggc cccatcgagt ctattgggct
     1441 gatcagtatt cgagaaatgt ataactcact gggagtgccc gtccctgcaa tgatggagag
     1501 cattagatgc ctgaaagaag ccagcctgtc cctgctggac gaagaggacg ccaacgagac
     1561 cgcaccctac tttgattaca ttattaaggc tatgagccga ccggttcacg tgggaagtgg
     1621 ggctacaaac ttctcactgc tgaaacaggc cggggatgtg gaggaaaacc caggaccaca
     1681 cgtgactagt gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1741 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1801 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1861 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1921 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1981 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2041 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2101 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     2161 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     2221 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2281 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     2341 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2401 caagtaagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     2461 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2521 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2581 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2641 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2701 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2761 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2821 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2881 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2941 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3001 cctttgggcc gcctccccgc atcgataccg tcgacccggg cggccgcttc gagcagacat
     3061 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3121 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3181 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     3241 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc tcccctagag
     3301 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     3361 atggagttgg catcctctct tttgggcggg tctcc
//
