LOCUS       Exported                3452 bp ds-DNA     linear   UNA 04-MAR-2022
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        495..772
                     /label=chicken beta-actin promoter
     regulatory      1193..1198
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1199..1654
                     /codon_start=1
                     /label=BDFP1.6
                     /translation="MANREVVETLKELLADGEKRVQVAGVIGTNAAEVVKTAVSLLFQE
                     YPELVSPGGCAYTTRRYNMCVRDMNYFLRMCSYAIVAGGASVLDGRMLAGFRDTFNSLG
                     IPLCPAARGIQLMKKIVKEKLATAGMTNIAFVDEPFDYIARVISETEI"
     CDS             1679..1735
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1748..2464
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    2489..3077
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2960..2971)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3120..3241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cagtaccatg acgtcaggcg gccatcgtgg tacgccgatc cgtgccggtc gctgattatt
       61 atgcagctgc ccgctcgctc gctcactgag ccgccgggca aagcccgggc gtcgggcgac
      121 tttgttcgcc cgccctcagt gaccgaccga accgccccag agaggagtgc actcatcact
      181 agggtctgta gtaatgatta acccgccatg ctactatcta cgtagccatg ctctaggaag
      241 agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      481 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
      541 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
      601 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
      661 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
      721 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      781 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      841 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      901 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      961 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg
     1021 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1081 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1141 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccggta ccgccaccat
     1201 ggccaatcgc gaggttgttg agaccctgaa ggagctcctg gctgacgggg agaagagagt
     1261 gcaggtggct ggagtgattg ggactaacgc tgccgaagtt gtcaagaccg ccgtgtcact
     1321 tctgtttcag gagtacccag agttggtgtc ccctggcggg tgtgcctata caacaaggcg
     1381 ctacaacatg tgtgtgaggg acatgaacta cttcctcagg atgtgtagct acgccatcgt
     1441 ggccgggggc gccagcgtct tggatggaag gatgctggcc gggttccggg atacattcaa
     1501 ctccctcggc atccctctgt gccccgctgc ccggggcatc caactgatga agaagatcgt
     1561 caaagagaaa ctggcgacag ccggcatgac taacattgca tttgtcgatg agccctttga
     1621 ctacatcgcc cgtgtgatta gcgaaacgga aattcgaccg gttcacgtgg gaagtggggc
     1681 tacaaacttc tcactgctga aacaggccgg ggatgtggag gaaaacccag gaccacacgt
     1741 gactagtgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1801 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1861 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1921 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1981 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2041 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2101 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2161 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2221 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2281 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2341 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2401 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2461 gtaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2521 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2581 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2641 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2701 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2761 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2821 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2881 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2941 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3001 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3061 ttgggccgcc tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat
     3121 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3181 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3241 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     3301 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatctcc cctagagcat
     3361 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     3421 gagttggcat cctctctttt gggcgggtct cc
//
