LOCUS       pINSPECT_5'xrRNA_NLuc_3'XAP1        9509 bp    DNA     circular UNA 28-DEC-2022
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...6et-dzu71gd
VERSION     urn.local...6et-dzu71gd
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(complement(<7304..7364),complement(460..>661))
                     /standard_name="lacZ'"
     misc_feature    662..667
                     /note="Geneious type: motif"
                     /standard_name="SD"
     misc_RNA        729..781
                     /standard_name="5'UTR_ß-globin"
     misc_feature    782..859
                     /note="Geneious type: motif"
                     /standard_name="xrRNA1"
     misc_feature    944..1009
                     /note="Geneious type: motif"
                     /standard_name="xrRNA2"
     gene            complement(1064..1097)
                     /note="Geneious type: Gene"
                     /standard_name="loxP; loxH"
     gene            complement(1168..1201)
                     /note="Geneious type: Gene"
                     /standard_name="loxP; loxH"
     source          <1235..>1436
                     /organism="Mason-Pfizer monkey virus"
                     /proviral
                     /mol_type="genomic RNA"
                     /db_xref="taxon:11855"
     misc_feature    1238..1407
                     /note="Geneious type: Comment"
                     /standard_name="CTE motif (Wodich 2000)"
     source          <1445..>1646
                     /organism="Mason-Pfizer monkey virus"
                     /proviral
                     /mol_type="genomic RNA"
                     /db_xref="taxon:11855"
     misc_feature    1448..1617
                     /note="Geneious type: Comment"
                     /standard_name="CTE motif (Wodich 2000)"
     polyA_signal    complement(1653..1700)
     polyA_signal    complement(1709..1929)
     source          complement(<1709..>1929)
                     /organism="Macaca mulatta polyomavirus 1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1891767"
     polyA_signal    complement(1936..2043)
     source          complement(<1936..>2043)
                     /organism="Oryctolagus cuniculus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9986"
     exon            complement(<2044..2073)
                     /note="beta-1 globin"
                     /number=3
     source          complement(<2044..>2113)
                     /organism="Oryctolagus cuniculus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9986"
     misc_feature    complement(2074..2076)
                     /note="Geneious type: Comment"
                     /note="Oriinal location: 1752..1753"
                     /note="Geneious type: motif"
                     /standard_name="SA"
     misc_feature    complement(2074..2113)
                     /note="Geneious type: region"
                     /standard_name="Rabbit Beta Globin Splice Acceptor"
     gene            2133..2166
                     /note="Geneious type: Gene"
                     /standard_name="loxP; loxH"
     misc_feature    complement(2223..4172)
                     /note="Geneious type: ORF"
                     /standard_name="ORF 5 (frame 3)"
     misc_feature    complement(2226..2317)
                     /note="Geneious type: region"
                     /standard_name="Cryptic peptide"
     gene            2237..2270
                     /note="Geneious type: Gene"
                     /standard_name="loxP; loxH"
     misc_feature    2284..2317
                     /note="Geneious type: region"
                     /standard_name="FRT F3 mutant"
     misc_feature    complement(2319..2384)
                     /note="Geneious type: region"
                     /standard_name="GSG-P2A"
     misc_feature    complement(2385..3509)
                     /note="Geneious type: region"
                     /standard_name="HSV thymidine kinase"
     misc_feature    complement(3510..3575)
                     /note="Geneious type: region"
                     /standard_name="GSG-P2A"
     misc_feature    complement(3576..4172)
                     /note="Geneious type: region"
                     /standard_name="Puromycin N-acetyltransferase"
     promoter        complement(4199..5386)
     misc_feature    5387..5420
                     /note="Geneious type: region"
                     /standard_name="FRT F3 mutant"
     misc_feature    5455..6034
                     /note="Geneious type: IRES"
                     /standard_name="EMCV IRES"
     misc_feature    6043..6558
                     /note="Geneious type: ORF"
                     /standard_name
     misc_feature    6043..6555
                     /note="Geneious type: region"
                     /standard_name="NanoLuc luciferase"
     source          complement(<6565..6711)
                     /organism="Oryctolagus cuniculus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9986"
                     /chromosome=1
                     /sex="female"
     misc_RNA        6612..6628
                     /standard_name="5'_complementary"
     misc_RNA        6629..6647
                     /standard_name="XAP1"
     misc_RNA        6648..6664
                     /standard_name="3'_complementary"
     misc_feature    6725..6925
                     /note="Geneious type: region"
                     /standard_name="CTE from Patent of RTEm26"
     misc_feature    6960..7160
                     /note="Geneious type: region"
                     /standard_name="CTE from Patent of RTEm26"
     misc_feature    7294..7296
                     /note="Geneious type: motif"
                     /standard_name="SA"
     rep_origin      7706..8373
     misc_feature    complement(8521..9381)
                     /note="Geneious type: ORF"
                     /standard_name="ORF 118 (frame 3)"
     misc_feature    complement(8524..9381)
                     /note="Geneious type: misc_marker"
                     /standard_name="Amp(R)"
     misc_feature    complement(8647..8674)
                     /note="Geneious type: primer"
                     /standard_name="pBKS_dual_BsaI_1_rv"
     primer_bind     complement(8647..8664)
                     /standard_name="64-L654"
     misc_feature    8654..8681
                     /note="Geneious type: primer"
                     /standard_name="pBKS_dual_BsaI_2_fw"
     misc_feature    8654..8674
                     /note="Geneious type: Comment"
                     /standard_name="Gibson Overlap"
     misc_feature    8665
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     misc_feature    8665
                     /note="Geneious type: Comment"
                     /standard_name="C->T to get rid of BsaI"
     primer_bind     8666..8681
                     /standard_name="70-L654"
ORIGIN      
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggaggtctc
      661 tgtaagtatc ctttttacag cacaacttaa tgagacagat agaaactggt cttgtagaaa
      721 cagagtagac acttgctttt gacacaactg tgtttacttg caatccccca aaacagacag
      781 atagtcaggc cagccggtta ggctgccacc gaaggttggt agacggtgct gcctgcgacc
      841 aaccccagga ggactgggtt accaaagctg attctccacg gttggaaagc ctcccagaac
      901 cgtctcggaa gaggagtccc tgccaacaat ggagatgaag cccgtgtcag atcgcgaaag
      961 cgccacttcg ccgaggagtg caatctgtga ggccccagga ggactgggta aacagtcgac
     1021 ccgggcggcc gcattttatc gaattgcaac ctcttcgagg gacataactt cgtataatgt
     1081 atgctatacg aagttatggg tcgatgctga gatctggact agagggtcga tggtgatgct
     1141 tggatcaaaa taggaagaat cccaatgata acttcgtata aagtatccta tacgaagtta
     1201 tactagaatt gcgtaccgcc accatgtcgc caaggccacc tcccctgtga gctagactgg
     1261 acagccaatg acgggtaaga gagtgacatt tctcactaac ctaagacagg agggccgtca
     1321 aagctactgc ctaatccaat gacgggtaat agtgacaaga aatgtatcac tccaacctaa
     1381 gacaggcgca gcctccgagg gatgtgtctt ttgtttttta taattaaaaa gggtgacacg
     1441 tgaggccacc tcccctgtga gctagactgg acagccaatg acgggtaaga gagtgacatt
     1501 tctcactaac ctaagacagg agggccgtca aagctactgc ctaatccaat gacgggtaat
     1561 agtgacaaga aatgtatcac tccaacctaa gacaggcgca gcctccgagg gatgtgtctt
     1621 ttgtttttta taattaaaaa gggtgaagcc gacacacaaa aaaccaacac acagatctct
     1681 agagctctgg atcttttatt gcccgggcta ccacatttgt agaggtttta cttgctttaa
     1741 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     1801 acttgtttat tgcagcttat aatggttaca aatatagcaa tagcatcaca aatttcacaa
     1861 atatagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1921 atcatgtctg tatactttaa atgatttgcc ctcccatatg tccttccgag tgagagacac
     1981 aaaaaattcc aacacactat tgcaatgaaa atacatttcc tttattagcc agaagtcaga
     2041 tgccagcaca acaaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac
     2101 atagttagca gagttacgta gcgatcgcta gtataacttc gtatagcata cattatacga
     2161 agttatgggt cgatggtgac aatagcgaaa aagaaagaac aatcaagggt ccccaaactc
     2221 acctaggtcc tttgatataa cttcgtatag gatactttat acgaagttat ggcatctcct
     2281 tcggaagttc ctattctatt tgaagtatag gaacttccag gtccaggatt ttcctcgacg
     2341 tcccctgcct gcttgagcag tgaaaagtta gttgccccgg atccgttggc ttcgcccatc
     2401 tctctagcga aggttctggc gaggtcacag atggtgggga tgctgccagg ggttgtcaca
     2461 tgggtctgca ccatgccgct tgtcagttgc agcagggcat ctctgcatcc ggctgggctc
     2521 tgatcgtagt ccaggatgaa cacatgcatg ggccgcagtc tttttgccag cacgtcgaga
     2581 gcccaggcga acacgttata caggtcgccg ttaggggcca gcagttcagg ggctctgaac
     2641 agtgtgaaca gggtgtcgcc gatgtgaggt ctgggtccag cgttagactg aggctcagcg
     2701 ccttgtggag gaactgctgt gccagagagc tgtccccagt cctctctcca ggaaccgccg
     2761 ccttgaagat agcgcacggt gttagccagc aggccgtaca ctcttctgat ggcggccagc
     2821 atagccagat ccagtctctc gccaggcctc tgtctcttag ccagtctgtc gatgtgtctg
     2881 tcctctggca gggctccaag cacgatgttt gtgccgggca gtgtaggagg aatcagagcc
     2941 acgaaagcca gcacggcctg aggtgtcatg ctgcccatca gataccgagc ggcagggtaa
     3001 cacagcaggg cgaagatggg gtgtctgtcg aagatcaggg tcagagcagg tggtggagcg
     3061 tgagaagatc cagcctcgcc gccaatatgt ggagccagca cagcgtctgt cacggcgtaa
     3121 ggcatgccca ttgtgatctg ggcagatgtc atgaccacag cagcgtcgcc tgcgctaatt
     3181 tcgccctggt ccagtctgtg ctgtgtggtg tagatgttgg cgattgtctc agaggctccc
     3241 aggacctgcc agtaggtcat aggctcgggc acgtacacga tgtcatctct gctgcccaga
     3301 gccaccagca gctgtgttgt tgtggtcttg cccatgccat gagggccgtc gatatacact
     3361 ctcagcagtg tgggcatctt ctgttccagc cgcacttctg tagcctcttg ctgccttctt
     3421 ggcctgaggg ctgttcttct gttgctgtgg cctctgcttc tagcggcttg atcgaaggcg
     3481 ctggcgtgct ggtgacaagg ataagaagca ggaccggggt tctcttccac gtcgccagcc
     3541 tgtttcagca ggctgaagtt tgtagcgccg gatccggcgc caggctttct ggtcatgcac
     3601 caggttctag ggccctcagg cacttccacg tcggcggtca cggtgaagcc cagtctctcg
     3661 tagaagggca ggtttctggg ggcgcttgtt tccaggaagg cgggcacgcc agccctttca
     3721 gcagcttcca ccccaggcag caccacagca gatcccagtc ccttgccctg gtggtcaggt
     3781 gacacgccca cggtggccag aaaccaggca ggctcttttg gtctgtgggg ggccagcagg
     3841 ccttccatct gctgctgggc agccagtcta gagccgctca gctcggccat tctaggtccg
     3901 atctcggcga acacagcgcc ggcttccaca gactcagggg ttgtccacac agccacagcg
     3961 gcgccatcat cggccaccca cactttgccg atgtccaggc ccactctggt cagaaacagt
     4021 tcctgcagct cggtcactct ctcgatgtgc cggtcggggt ccacggtgtg tcttgtggca
     4081 gggtaatcgg cgaaggcagc ggccagtgtc cgcacagctc ttggcacatc gtccctggtg
     4141 gccagccgca ctgtgggctt gtactcggtc atggtggcgc gccttttagg ggtagttttc
     4201 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     4261 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     4321 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     4381 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     4441 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     4501 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag
     4561 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     4621 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     4681 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     4741 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     4801 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     4861 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     4921 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     4981 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     5041 cgggatcaag aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg
     5101 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     5161 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     5221 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     5281 cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact
     5341 gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caattcgaag ttcctattct
     5401 atttgaagta taggaacttc aaatacaagg gcttactgtg cggactggga cgcccccccc
     5461 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     5521 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5581 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5641 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5701 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     5761 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     5821 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     5881 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     5941 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
     6001 tggttttcct ttgaaaaaca cgatgataat atggccacca ccatggtctt tacactggaa
     6061 gatttcgtcg gcgactggcg gcagacagct ggctacaatc tggaccaggt gctggaacaa
     6121 ggcggcgtgt cctctctgtt tcaaaacctg ggagtgtctg tgacccctat ccagagaatc
     6181 gtgctgagcg gcgagaacgg cctgaagatc gacatccatg tgatcatccc ttacgagggc
     6241 ctgtccggcg atcagatggg ccagatcgag aagattttca aggtggtgta ccccgtggac
     6301 gaccaccact tcaaagtgat cctgcactac ggcaccctgg tcatcgatgg cgtgacccct
     6361 aacatgatcg actacttcgg cagaccctac gagggaatcg ccgtgttcga cggcaagaaa
     6421 atcaccgtga ccggcacact gtggaacggc aacaagatca tcgacgagcg gctgatcaac
     6481 cccgatggca gcctgctgtt cagagtgacc atcaacggcg tgacaggatg gcggctgtgc
     6541 gaaagaatcc tggcctaatg tacagatctt tttccctctg ccaaaaatta tggggacatc
     6601 atgaagcccc tgggttgcac acgtctcgag ggacgggcag ggctgggcga gacgtgtgca
     6661 accctgagca tctgacttct ggctaatata ggaaatttat tttcattgca agtacagata
     6721 tcactatcga taccgcgggg atcctctaga gtagaccacc tcccctgcga gctaagctgg
     6781 acagccaatg acgactaaga gagtgacatt tttcactaag ctaagacagg agggccgtca
     6841 gagctactgc ctaatccaaa gacggctaaa agtgataaaa atgtatcact ccaagttaag
     6901 acaggcgcag cttccgaggg atttgcctgg ccggattgac tgacgtctta accactgcat
     6961 atcgataccg cggggatcct ctagagtaga ccacctcccc tgcgagctaa gctggacagc
     7021 caatgacgac taagagagtg acatttttca ctaagctaag acaggagggc cgtcagagct
     7081 actgcctaat ccaaagacgg ctaaaagtga taaaaatgta tcactccaag ttaagacagg
     7141 cgcagcttcc gagggatttg cgtttaaacg cggccgctct atactcgacg attgactatc
     7201 tgacaaatca tagagaacat agagaacctc ctcgagtcgc ctgcttttct gcctggtact
     7261 aacttctctc ccctctcctc ttttcttttt ctgcagggag accgtaccca gcttttgttc
     7321 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7381 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7441 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7501 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7561 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8101 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8161 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8221 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8281 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8341 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8401 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8461 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8521 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8581 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8641 cagtgctgca atgataccgc gagatccacg ctcaccggct ccagatttat cagcaataaa
     8701 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8761 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8821 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8881 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8941 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9001 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9061 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9121 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9181 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9241 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9301 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9361 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9421 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9481 tccgcgcaca tttccccgaa aagtgccac
//
