LOCUS       pAC14        8359 bp DNA     circular SYN 21-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...86-82xpuqh
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..98
                     /label=ConS'
     primer_bind     1..20
                     /label=AC_09
     primer_bind     complement(33..52)
                     /label=AC_019
     promoter        103..161
                     /label=CP25
     RBS             172..179
     misc_feature    188..904
                     /label=GFP-mut3
     terminator      909..991
                     /label=rpoC
     primer_bind     complement(993..1034)
                     /label=AC_037
     misc_feature    996..1014
                     /label=ConE'
     primer_bind     1015..1032
                     /label=AC_036
     terminator      1026..1122
                     /label=AmpR Terminator
                     /label=AmpR\Terminator
     CDS             complement(1120..1980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1123..1980)
                     /label=AmpR
     primer_bind     complement(1190..1210)
                     /label=AC_08
     promoter        complement(1981..2085)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(2022..2050)
                     /label=amp prom
                     /label=amp\prom
     primer_bind     complement(2113..2135)
                     /label=AC_010
     primer_bind     2113..2135
                     /label=AC_018
     misc_feature    2136..8359
                     /label=pTF-FC2
     primer_bind     2136..2155
                     /label=AC_051
     misc_feature    2270..2599
                     /label=oriV
     repeat_region   2400..2414
     repeat_region   2419..2435
     repeat_region   2447..2453
     repeat_region   2459..2465
     repeat_region   2526..2547
     repeat_region   2548..2569
     CDS             complement(2556..3455)
                     /codon_start=1
                     /transl_table=11
                     /gene="repC"
                     /product="replication protein C"
                     /label=repC
                     /protein_id="AAA27382.1"
                     /translation="MEQEKKGESAMSYDLTHARHDPAHCLTPGLFRSLKRGERKRLKLD
                     VTYNYGDDSIRFWGPEPLGGDDLRVLQGLVAMAAISGDNGRGIVLRHETESEAGQQLRL
                     WLDMRWDAIEKDTMVAKGSFRQLARELGYAEDGGSQFKTIRESIERLWAVSVIVERGGK
                     RQGFRILSEYASDEQEGKLFVALNPRLADAVMGERPHTRINMAEVRKLETDPARLLHQR
                     LCGWIDPGKSGKAEIDTLCGYVWPDAANDEAMKKRRQTARKALVELAAVGWTVNEYAKG
                     KWEISRPNPRRNVPQPPA"
     gene            complement(2556..3455)
                     /gene="repC"
                     /label=repC
     repeat_region   complement(2570..2591)
                     /gene="repC"
     primer_bind     complement(3403..3422)
                     /label=AC_056
     CDS             complement(3415..4287)
                     /codon_start=1
                     /transl_table=11
                     /gene="repA"
                     /product="replication protein A"
                     /label=repA
                     /protein_id="AAA27381.1"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFALE
                     AAMSIACSVAGGDLVGLTPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIAEN
                     LMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVSVLE
                     HIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQEAERM
                     SDRGFDRSPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHSGGVLLPVELQEAISNGAG
                     KKGGKRNEL"
     gene            complement(3415..4287)
                     /gene="repA"
                     /label=repA
     primer_bind     complement(4138..4157)
                     /label=AC_055
     CDS             complement(4353..4568)
                     /codon_start=1
                     /label=pasC / ORF5
                     /translation="MIEYSYQIDPRPSDLGGGWRLRLLESGEEVGGGVFPLSEYATAEN
                     AEEAASYAYEDALAEASAWLASRGEN"
     CDS             complement(4360..4371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4568..4837)
                     /codon_start=1
                     /transl_table=11
                     /label=pasB / ORF4
                     /translation="MAWKVELDPAAERELGKIDQQTARRILAFLHGRVAQLDDPRSIGE
                     ALKGSKLGAFWKYRVGDWRIIASIEDGALRILVMRIGNRKEVYRQ"
     RBS             4578..4586
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4821..5045)
                     /codon_start=1
                     /transl_table=11
                     /label=pasA / ORF3
                     /translation="MLAIRLPAEVETRLEALAQATGRTKTFYAREAILEHLDDLEDLYL
                     AEQRLIDIRAGKTQTVPLEEVMKRYGMEG"
     primer_bind     complement(5034..5053)
                     /label=AC_054
     CDS             complement(5102..6163)
                     /codon_start=1
                     /transl_table=11
                     /gene="repB"
                     /product="replication primase"
                     /label=repB
                     /protein_id="AAA27386.1"
                     /translation="MKSEQLKQFERYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGI
                     TRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIKDGYQPA
                     AVLESSPGNYQAIITVSKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
                     KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALE
                     LAAASGSAIDAYQRHYRDVLKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCA
                     PATRQKDEGRDWNDYAQRTARYAYSAAGDRQAADLGKYRQQWEKLEGREPVRQQEQAKA
                     QKIERDNSPGMSL"
     primer_bind     5866..5885
                     /label=AC_049
     primer_bind     complement(5866..5885)
                     /label=AC_050
     regulatory      6175..6180
                     /gene="repB"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(6374..7600)
                     /codon_start=1
                     /transl_table=11
                     /gene="mobA"
                     /label=mobA
                     /note="ORF6"
                     /protein_id="AAA27389.1"
                     /translation="MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELG
                     VKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQ
                     REQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKA
                     QTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPQR
                     QQVAQREPEPIKPDVPGWKDYITGRKAHYSEKNADKLVQDKRQEQERKQLAEQQKARRD
                     ELMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRM
                     QKSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEGGRGGGV
                     SFVDKGKSID"
     gene            complement(6374..7600)
                     /gene="mobA"
                     /label=mobA
     primer_bind     complement(6643..6662)
                     /label=AC_053
     primer_bind     complement(7528..7547)
                     /label=AC_052
     oriT            8213..8267
     primer_bind     complement(8340..8359)
                     /label=AC_048
ORIGIN
        1 atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat taacccacag
       61 tacagacact gcgacaacgt ggcaattcgt cgcaatacaa cgctttggca gtttattctt
      121 gacatgtagt gagggggctg gtataatcac atagtactgt tatacagaaa cagaggagat
      181 attacatatg agtaaaggag aagagctttt cacaggagtt gtcccaatcc tcgtggaatt
      241 agacggtgat gttaatgggc acaagttctc tgtcagtgga gagggtgaag gcgacgcaac
      301 atatggcaag ctgaccctta aatttatttg caccacgggt aaactacctg ttccatggcc
      361 aacactggtc actacgttcg ggtatggggt tcagtgcttt gcgcgctacc cagatcacat
      421 gaaacagcac gactttttca agagtgcaat gcccgaaggc tatgtacagg agagaaccat
      481 cttttttaag gatgacggca actataagac acgcgccgaa gtgaagttcg agggtgatac
      541 ccttgttaat agaatcgagt taaagggtat tgactttaag gaagatggaa atattttagg
      601 ccacaaactg gaatataact ataactccca taatgtgtac attatggccg acaagcaaaa
      661 gaacggtatc aaggttaact tcaagatcag acacaacatt gaggatggaa gcgttcaact
      721 agccgaccat taccaacaaa acaccccaat tggcgatggg cctgtgctgt taccagacaa
      781 ccattacctg tccactcaat ctgccctttc gaaagatccc aacgaaaagc gcgaccacat
      841 ggtccttctt gagtttgtca cggctgctgg gattacacac ggcatggatg aactatacaa
      901 ataaatccgt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
      961 tggggggagt ttagggaaag agcatttgtc agctggaaat ctgctcgtca gtggcgagcg
     1021 gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1261 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2101 agtgccacct gtcatgacca aaatccctta acgtgctcaa ggcagccaga acatccaaaa
     2161 atccgctgta ggcgattctg atggcttctg ttcgggtatg aggcgatgtg ctccacaagc
     2221 acgaccgaca agccagccga aacgctcgcc aagctggccg ctgcgctacc tgccggagag
     2281 gccaaacgct accgggtgat ggtgtccatt gtcccggtgg aagaatgaag gcgagcgggg
     2341 cggggcgggg tgtgtctggc cgggcttgtc ccggccttgt cttttggggt ttttggtaag
     2401 gacttttgac gccctcaagg gcggcgaaaa gtaccgcgcc tagtggatga tcttggatag
     2461 atcatggagg gattttgcga atcgcccgca aacccgcgcc gttgctaggt ttttcagatt
     2521 tcggcgggga acgttacgcc gggggttggg gaacgttacg ccgggggttg gggaacgtta
     2581 cgccgggggt tgggcctgct gatttcccac ttgcccttgg cgtactcgtt caccgtccaa
     2641 ccaacggcgg caagctcgac aagcgccttg cgggcggtct ggcggcgctt tttcattgct
     2701 tcatcgttgg ctgcgtctgg ccatacataa ccgcacagcg tgtcgatttc agctttgcca
     2761 gactttccgg ggtcaatcca gccacatagc cgctggtgta gcagccgtgc cgggtctgtt
     2821 tccagcttgc gaacttctgc catgttgatg cgggtgtgcg ggcgctctcc cataaccgca
     2881 tccgccagcc ggggattaag cgcaacaaat aacttgcctt cttgctcgtc gctcgcgtac
     2941 tcggacagaa tgcggaaccc ttgccgctta ccacctcttt cgacaatcac cgataccgcc
     3001 caaagccgtt cgatgctttc ccggatggtt ttaaactgac ttcctccatc ttcggcgtag
     3061 ccaagttctc gggccaactg gcggaagctg cccttggcta ccatcgtatc tttctctatg
     3121 gcgtcccacc gcatatcaag ccataggcgg agttgctggc ctgcttctga ttccgtttcg
     3181 tgccgtagca cgatgccgcg cccgttatct ccggaaattg cagccattgc caccagccct
     3241 tgcaatacgc gcaagtcatc gccgccaagt ggttcaggcc cccaaaaacg gattgagtca
     3301 tctccgtagt tgtaggtcac atcgagcttg agcctctttc gttctccgcg cttgagactg
     3361 cggaaaagcc ccggcgtgag gcaatgcgcg gggtcgtgcc gcgcatgggt gaggtcatag
     3421 ctcattgcgc tttccccctt tttttcctgc tccattgctg attgcctcct gtagttcaac
     3481 gggcaacaac accccgccgc tgtgccgctg ataccagcgg tctagcgggg tcgcgtcgta
     3541 gttctgcttg ctcacgccaa agcggacaaa aaggccgcgc cgctcgttgc cgatgggcga
     3601 acgatcaaag ccccggtcac tcatgcgctc ggcttcctgc tccgtcattt tggcgacata
     3661 gccgcaccat ctggcgttgt cgatcagggc agacgcgccc cgcgctgcct gctgctggtc
     3721 ggtctggccg tcgcgggcgc tgcccttgtt gacgtggtgc aggtacagga cagccgcccc
     3781 ggtggtcgcc gcgatgtgtt ccaacacgga aacaaggtgg gccatgtcgc cattgctgtt
     3841 ctcgtcgagg ctgtgaatcc ggctcagggt gtccagcaca atcagccggg cccctgcgct
     3901 gtagtcgatg acgcggcgca agtgcgcctc gtccatcacg tttagccgct tgcccatgat
     3961 cggctcaagc atcaggttct cagcgatggc ttcgcgggcc gactggttga ggtgctggcc
     4021 gatggcgtgg acacgtcgca caagggcggg ctgtggatct tcgccagcga gataaaccac
     4081 gcgcccggtg tgcgccgggg ttagtcccac aaggtcgccg cctgccacac tgcaagcgat
     4141 tgacatggcc gcttcaagag caaaaaagct cttgccagtt gcgccaggtg cgacaagtgc
     4201 gcccacggtt ccggccaaga atccgggcca gatgaaatca agttctggcg gctcattggt
     4261 gaaggccgcc ataatgtcta aagccatgct tgtctccttt tcagacagtt gtggttaggc
     4321 gctgccgggg ccgcaatccc ctgccgcgcc gctcaatttt cgcccctcga tgccagccac
     4381 gccgaagcct cggccaaggc gtcctcatag gcgtagctgg ccgcttcttc tgcgttctct
     4441 gctgtggcgt actcggacaa cgggaacact ccgccgccga cttcctcgcc gctttccaac
     4501 aggcgcaacc gccagccgcc gccaaggtcg gaggggcgcg ggtcgatctg gtagctgtat
     4561 tcgatcattg gcggtaaacc tccttacgat tgccgatgcg cataacgagg atgcgcaaag
     4621 caccgtcctc gatgctggcg atgattcgcc aatccccaac gcggtatttc cagaaggctc
     4681 ccagtttgga gcctttgagg gcttcgccaa tgctgcgcgg gtcgtcgagc tgggcgacac
     4741 ggccatgcaa aaaagcgagg atgcggcggg cggtctgctg gtcgatcttg cctagctcgc
     4801 gctcggcggc tgggtcgagt tcaaccttcc atgccatagc gtttcatcac ttcttcgagt
     4861 ggcacggttt gggttttgcc tgcgcgaatg tcgatcaggc gttgctctgc aaggtacaaa
     4921 tcttcgaggt catccaagtg ctcaaggatg gcttcgcggg catagaaagt cttggtccgc
     4981 cctgtggcct gcgccagtgc ttcaaggcgg gtttccactt cggcgggcag tcggattgca
     5041 agcatgtttc ggctcctgtt ctgaaaacgc tatacaagta tatcacaacc accacgcaac
     5101 gctagagact cattcccggc gagttgtcgc gctcgatctt ctgcgccttt gcctgctcct
     5161 gctgtcgtac aggctcgcgc ccttccagct tctcccactg ctgccggtac ttgccaagat
     5221 cggcggcttg ccggtcgcct gcggcgctgt aggcatagcg ggcggtgcgc tgcgcgtagt
     5281 cgttccaatc gcggccctcg tctttctgcc gggtggccgg tgcgcactgg cggatagcgc
     5341 cctcgatggc cgcttgatcg tggccggtga cgcgcatacg cacggcaatc atggaatcca
     5401 cgcgggacaa gtccacctcg ccgccacgct gccgcttgag cacgtcgcgg taatgccgct
     5461 ggtaggcgtc gatcgcgctg ccgctggccg ctgcaagctc caaggcgggc ttggctttgc
     5521 tgcgctcggg ctgctgcgcc ttcaaggccg cttgccgctg atactcggcg tcgatctggc
     5581 tggacaaggc cagcgccttg acgcactccc ggcgctcggc cttgagcaag cgcacctctg
     5641 gatagctgcc gtcctcccgc tggtgcttgg gcttgcggtt ctcgtagccg ggtgcgcggt
     5701 gcgggtggat ggctcccgat agcttcgggt cgccgtattc acggttcagg gcatcgctca
     5761 ggcggttgcc cacgtcctta tcgtgggccg tccccagctt cgacacggtg atgatggcct
     5821 gatagttgcc ggggctggat tccagcacgg cggcgggctg gtagccgtct ttgataagcc
     5881 gctccagctt ctcccggttc atgtcgtcga tgaggatgtg atgcttcttg tccgatagcg
     5941 gcgtgtagta gaggttttcg ccccggcgct gtaggcgctg catctccggc gtgcgctgct
     6001 cgatctcctg cggtgtgaaa ccccgcgtga tgccgtcctt cttgtcgagg atgaaggttt
     6061 gcttccttcc gtctgcctgc atcttgatgg acgttacccg gtagcgctcc gcgcctaccg
     6121 cttccgcgta ccgctcgaac tgctttagct gctccgattt catcgcctgc gccctctcct
     6181 gctgtatccg ctgccgctcc tgctggatgc tctcctgaag ctctgcattt gtgatcttga
     6241 agccgtgctc tgctgctaac ttcccgcaca tggccttgta ttcgtcgttc ccggttacgg
     6301 tgaagctgcc ccacttctgc gccgatagct gcaaggcggc aagcgtgctg tcccggttgc
     6361 gccagtcgtg aatatcgatg cttttgccct tgtccacgaa agacacaccc ccgccccgcc
     6421 ccccttcttc cttccggctg taatgcacct gctggcccac gatttcaggc tgataggcgc
     6481 gaatgtcgcg cggcgtcggc ggctcgctgc ggtcgccctc gatgcgctgc ggctcgctgg
     6541 cccggtgtcg ccactgctcg gcaagctccg ggcttttctg catccgctgc cactgctcca
     6601 ggtcgggata gggtctgaac tgctgccggt gctgctcgcg ctgcttctgg tgcttctcct
     6661 tcaaggcggc tttctctgcg gcctgctcgg cggcaatcac gctgcgcatg gcgttgagca
     6721 cttcgccctt gcctttccag ttgccgcgca tgagttcatc gcggcgggcc ttctgctgct
     6781 cggcaagttg cttgcgctcc tgctcctgcc gcttgtcctg taccagcttg tccgcgttct
     6841 tctccgagta gtgcgccttg cgcccggtga tgtagtcctt ccatcccggc acgtctggct
     6901 tgatcggctc cggctcgcgc tgtgctacct gctgacgctg cggcggctgg taagctccga
     6961 gtcgcttttg caacttggat aggctcgcgt cccggtctgc gctgctggcc ttcacgccaa
     7021 catcaccgac aaagagcgtc gccccgctgc ctgttttctc gtagcgcatc cccttggctg
     7081 ctaactcgcg gtgcaactgc tcccacgtct gcgccttttt gatgatcggt gcgccgtcct
     7141 caatggcgat gcgttcggcg gatttctccc cggtgcggtt ttccatgtcg cgcttcggct
     7201 gggctggctg gcgcggcttg tccttgtcga tgtgctcccg gcctaactca ccattctcca
     7261 acacctgata acgcccgttc tgctcacgct gccagccttg tgcgttctcg attcgtgcta
     7321 tcgccttgtg cgctgcctca atgtcgaagc cgttattgat cttcaccacc ttgagcgttt
     7381 caggatgcac gcggttgatg gcaaggtgca ggtgcaagtt gtccgtgtcg gcgtgcaaac
     7441 catagatggc ttgatggtct ttaacaccta gctcatccat gaaaatgctt accgcttctt
     7501 cgacctgctc cgggctgggc tgctcgcctt ctcgccaact caggacatag tgattgatcg
     7561 tatccttgct tcgcactgcc tcttgtgaca aggcaatcat ttctgccgtc tggctctgtg
     7621 gttcatcggt gatgaatcca cgcgccccgg catggatgca cttctcctgc gagttctcac
     7681 gctcaggctc acggatgtag ccagttaggc gggaaacgcg aaccgccttg cttgctgcct
     7741 tctgcgggtt cttaaccttc ttgacgatca cggctcagtt tcccgatata ggctttcaag
     7801 accacaagcg ccccggctgt gtccttgctg tacacgcctc cgctctcgtt gtggatgtgc
     7861 ttaagcaatc caccgatgcg gcgcaactct ttgagcatta ccgcgtcggc gctggcaatg
     7921 atcggcctgc caaagtagcg gcggcggaca agctccgaca tgctcaaccc ggcaaggtcg
     7981 gcatcctctt tcaagcgggc cttctccgct gcggtcagcc gtacattgat aacggcgtcc
     8041 agcggttccg gcccctgcgt ttcaaatggc atcgtgtctc cttctcgcgg ggggcgggcg
     8101 gggtgtgtct tggacagtaa gaagcgagag cgccgcactg tccggggtca aggggatacc
     8161 ccttgcagga taagccagac cgtgagaagc gaagcgccga atgggcaggc gtccgttgga
     8221 accacgtggc gaagccatgt gtgttacaac ggtcatcctg tattgctcaa ccgctctact
     8281 atcatatcag cagtttaggc acactagatg cactttgcaa caagcataaa aggcgctatg
     8341 ctgtcaactc cgaaaccag
//
