LOCUS       pAC23        4876 bp DNA     circular SYN 21-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...86-82xpuqh
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          90..2890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..89)
                     /label=AC_010
     misc_feature    70..2910
                     /label=pBBR1
     primer_bind     70..109
                     /label=AC_011
     CDS             complement(136..801)
                     /codon_start=1
                     /product="replication protein for the 
                     broad-host-rangeplasmid pBBR1 from Bordetella 
                     bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     rep_origin      802..1571
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range 
                     plasmidpBBR1 from Bordetella bronchiseptica; requires the 
                     pBBR1 Rep protein for replication"
     primer_bind     complement(1670..1688)
                     /label=AC_013
     promoter        1676..1727
                     /label=pMob
     oriT            1694..1716
     CDS             1795..2814
                     /codon_start=1
                     /label=mob
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     primer_bind     2662..2676
                     /label=AC_036
                     /note="FZ-01"
     terminator      2822..2858
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     primer_bind     complement(2872..2910)
                     /label=AC_012
     misc_feature    2891..2926
                     /label=ConS'
     primer_bind     2891..2910
                     /label=AC_09
     primer_bind     complement(2891..2910)
                     /label=AC_013
     primer_bind     complement(2907..2946)
                     /label=AC_043
     primer_bind     2927..3015
                     /label=AC_042
     promoter        2927..2985
                     /label=CP25
     RBS             2996..3003
     misc_feature    3012..3728
                     /label=E2-crimson
     terminator      3733..3815
                     /label=rpoC
     primer_bind     complement(3825..3864)
                     /label=AC_041
     primer_bind     3845..3862
                     /label=AC_036
                     /note="FZ-01"
     terminator      3856..3952
                     /label=AmpR Terminator
                     /label=AmpR\Terminator
     CDS             complement(3950..4810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3953..4810)
                     /label=AmpR
     primer_bind     complement(4020..4040)
                     /label=AC_08
     promoter        complement(join(4811..4876,1..39))
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(join(4852..4876,1..4))
                     /label=amp prom
                     /label=amp\prom
ORIGIN
        1 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
       61 acctgtcatg accaaaatcc cttaacgtga cgcccggtag tgatcttatt tcattatggt
      121 gaaagttgga acctcttacg tacggcgcgg cagcgtgacc cgtgtcggcg gctccaacgg
      181 ctcgccatcg tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgtcg
      241 ttcccattcc tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg
      301 ctggctgggc ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt
      361 cgggatgcgg cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac
      421 caccacggcg gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac
      481 gcggtcattg accacgtagg ccgacacggt gccggggccg ttgagcttca cgacggagat
      541 ccagcgctcg gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat
      601 gagcttggaa agtgtcttct ggctgaccac cacggcgttc tggtggccca tctgcgccac
      661 gaggtgatgc agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc
      721 atgcgctttg cgttccgttt gcacccagtg accgggcttg ttcttggctt gaatgccgat
      781 ttctctggac tgcgtggcca tgcttatctc catgcggtag ggtgccgcac ggttgcggca
      841 ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag
      901 tggcagtcaa ttacagattt tctttaacct acgcaatgag ctattgcggg gggtgccgca
      961 atgagctgtt gcgtaccccc cttttttaag ttgttgattt ttaagtcttt cgcatttcgc
     1021 cctatatcta gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt
     1081 cggcgcggct ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg
     1141 gccttcggcc ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga
     1201 ggctcggggg gcaggcgggc gggcttcgcc ttcgactgcc cccactcgca taggcttggg
     1261 tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc ttgacccgcc
     1321 ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga ggcttttccc
     1381 cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg gagccggtgg
     1441 gtatgtggtc gaaggctggg tagccggtgg gcaatccctg tggtcaagct cgtgggcagg
     1501 cgcagcctgt ccatcagctt gtccagcagg gttgtccacg ggccgagcga agcgagccag
     1561 ccggtggccg ctcgcggcca tcgtccacat atccacgggc tggcaaggga gcgcagcgac
     1621 cgcgcagggc gaagcccgga gagcaagccc gtagggcgcc gcagccgccg taggcggtca
     1681 cgactttgcg aagcaaagtc tagtgagtat actcaagcat tgagtggccc gccggaggca
     1741 ccgccttgcg ctgcccccgt cgagccggtt ggacaccaaa agggaggggc aggcatggcg
     1801 gcatacgcga tcatgcgatg caagaagctg gcgaaaatgg gcaacgtggc ggccagtctc
     1861 aagcacgcct accgcgagcg cgagacgccc aacgctgacg ccagcaggac gccagagaac
     1921 gagcactggg cggccagcag caccgatgaa gcgatgggcc gactgcgcga gttgctgcca
     1981 gagaagcggc gcaaggacgc tgtgttggcg gtcgagtacg tcatgacggc cagcccggaa
     2041 tggtggaagt cggccagcca agaacagcag gcggcgttct tcgagaaggc gcacaagtgg
     2101 ctggcggaca agtacggggc ggatcgcatc gtgacggcca gcatccaccg tgacgaaacc
     2161 agcccgcaca tgaccgcgtt cgtggtgccg ctgacgcagg acggcaggct gtcggccaag
     2221 gagttcatcg gcaacaaagc gcagatgacc cgcgaccaga ccacgtttgc ggccgctgtg
     2281 gccgatctag ggctgcaacg gggcatcgag ggcagcaagg cacgtcacac gcgcattcag
     2341 gcgttctacg aggccctgga gcggccacca gtgggccacg tcaccatcag cccgcaagcg
     2401 gtcgagccac gcgcctatgc accgcaggga ttggccgaaa agctgggaat ctcaaagcgc
     2461 gttgagacgc cggaagccgt ggccgaccgg ctgacaaaag cggttcggca ggggtatgag
     2521 cctgccctac aggccgccgc aggagcgcgt gagatgcgca agaaggccga tcaagcccaa
     2581 gagacggccc gagaccttcg ggagcgcctg aagcccgttc tggacgccct ggggccgttg
     2641 aatcgggata tgcaggccaa ggccgccgcg atcatcaagg ccgtgggcga aaagctgctg
     2701 acggaacagc gggaagtcca gcgccagaaa caggcccagc gccagcagga acgcgggcgc
     2761 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgagcggaa
     2821 caaaactaaa gcgccacaag ggcgctttag tttgttttca gtccagttac gctggagtct
     2881 gaggctcgtc atctgaatca tgcgcggatg acacgaactc acgacgcttt ggcagtttat
     2941 tcttgacatg tagtgagggg gctggtataa tcacatagta ctgttataca gaaacagagg
     3001 agatattaca tatggatagc actgagaacg tcatcaagcc cttcatgcgc ttcaaggtgc
     3061 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgtgggc gagggcaagc
     3121 cctacgaggg cacccagacc gccaagctgc aagtgaccaa gggcggcccc ctgcccttcg
     3181 cctgggacat cctgtccccc cagttcttct acggctccaa ggcgtacatc aagcaccccg
     3241 ccgacatccc cgactacctc aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
     3301 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca
     3361 ccctcatcta ccacgtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc
     3421 agaagaagac tctgggctgg gagccctcca ctgagcgcaa ctacccccgc gacggcgtgc
     3481 tgaagggcga gaaccacatg gcgctgaagc tgaagggcgg cggccactac ctgtgtgagt
     3541 tcaagtccat ctacatggcc aagaagcccg tgaagctgcc cggctaccac tacgtggact
     3601 acaagctcga catcacctcc cacaacgagg actacaccgt ggtggagcag tacgagcgcg
     3661 ccgaggcccg ccaccacctg ttccagactc acggtatgga cgaattgtac aagcacgacg
     3721 aattgtaaat ccgtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
     3781 tttatggggg gagtttaggg aaagagcatt tgtcagctgg atcagcagat gggaaccgga
     3841 tactcgagcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4021 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4081 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4861 tgagcggata catatt
//
