LOCUS       pAC24        8300 bp DNA     circular SYN 21-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...86-82xpuqh
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..6225
                     /label=pTF-FC2 fragment
     primer_bind     1..20
                     /label=AC_051
     misc_feature    136..465
                     /label=oriV
     repeat_region   266..280
     repeat_region   285..301
     repeat_region   313..319
     repeat_region   325..331
     repeat_region   392..413
     repeat_region   414..435
     CDS             complement(422..1321)
                     /codon_start=1
                     /transl_table=11
                     /gene="repC"
                     /product="replication protein C"
                     /label=repC
                     /protein_id="AAA27382.1"
                     /translation="MEQEKKGESAMSYDLTHARHDPAHCLTPGLFRSLKRGERKRLKLD
                     VTYNYGDDSIRFWGPEPLGGDDLRVLQGLVAMAAISGDNGRGIVLRHETESEAGQQLRL
                     WLDMRWDAIEKDTMVAKGSFRQLARELGYAEDGGSQFKTIRESIERLWAVSVIVERGGK
                     RQGFRILSEYASDEQEGKLFVALNPRLADAVMGERPHTRINMAEVRKLETDPARLLHQR
                     LCGWIDPGKSGKAEIDTLCGYVWPDAANDEAMKKRRQTARKALVELAAVGWTVNEYAKG
                     KWEISRPNPRRNVPQPPA"
     gene            complement(422..1321)
                     /gene="repC"
                     /label=repC
     repeat_region   complement(436..457)
                     /gene="repC"
     primer_bind     complement(1269..1288)
                     /label=AC_056
     CDS             complement(1281..2153)
                     /codon_start=1
                     /transl_table=11
                     /gene="repA"
                     /product="replication protein A"
                     /label=repA
                     /protein_id="AAA27381.1"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFALE
                     AAMSIACSVAGGDLVGLTPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIAEN
                     LMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVSVLE
                     HIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQEAERM
                     SDRGFDRSPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHSGGVLLPVELQEAISNGAG
                     KKGGKRNEL"
     gene            complement(1281..2153)
                     /gene="repA"
                     /label=repA
     primer_bind     complement(2004..2023)
                     /label=AC_055
     CDS             complement(2219..2434)
                     /codon_start=1
                     /label=pasC / ORF5
                     /translation="MIEYSYQIDPRPSDLGGGWRLRLLESGEEVGGGVFPLSEYATAEN
                     AEEAASYAYEDALAEASAWLASRGEN"
     CDS             complement(2226..2237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2434..2703)
                     /codon_start=1
                     /transl_table=11
                     /label=pasB / ORF4
                     /translation="MAWKVELDPAAERELGKIDQQTARRILAFLHGRVAQLDDPRSIGE
                     ALKGSKLGAFWKYRVGDWRIIASIEDGALRILVMRIGNRKEVYRQ"
     RBS             2444..2452
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2687..2911)
                     /codon_start=1
                     /transl_table=11
                     /label=pasA / ORF3
                     /translation="MLAIRLPAEVETRLEALAQATGRTKTFYAREAILEHLDDLEDLYL
                     AEQRLIDIRAGKTQTVPLEEVMKRYGMEG"
     primer_bind     complement(2900..2919)
                     /label=AC_054
     CDS             complement(2968..4029)
                     /codon_start=1
                     /transl_table=11
                     /gene="repB"
                     /product="replication primase"
                     /label=repB
                     /protein_id="AAA27386.1"
                     /translation="MKSEQLKQFERYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGI
                     TRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIKDGYQPA
                     AVLESSPGNYQAIITVSKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
                     KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALE
                     LAAASGSAIDAYQRHYRDVLKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCA
                     PATRQKDEGRDWNDYAQRTARYAYSAAGDRQAADLGKYRQQWEKLEGREPVRQQEQAKA
                     QKIERDNSPGMSL"
     primer_bind     3732..3751
                     /label=AC_049
     primer_bind     complement(3732..3751)
                     /label=AC_050
     regulatory      4041..4046
                     /gene="repB"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(4240..5466)
                     /codon_start=1
                     /transl_table=11
                     /gene="mobA"
                     /label=mobA
                     /note="ORF6"
                     /protein_id="AAA27389.1"
                     /translation="MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELG
                     VKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQ
                     REQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKA
                     QTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPQR
                     QQVAQREPEPIKPDVPGWKDYITGRKAHYSEKNADKLVQDKRQEQERKQLAEQQKARRD
                     ELMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRM
                     QKSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEGGRGGGV
                     SFVDKGKSID"
     gene            complement(4240..5466)
                     /gene="mobA"
                     /label=mobA
     primer_bind     complement(4509..4528)
                     /label=AC_053
     primer_bind     complement(5394..5413)
                     /label=AC_052
     oriT            6079..6133
     primer_bind     complement(6206..6225)
                     /label=AC_048
     misc_feature    6226..6261
                     /label=ConS'
     primer_bind     6226..6245
                     /label=AC_09
     primer_bind     complement(6242..6281)
                     /label=AC_043
     primer_bind     6262..6350
                     /label=AC_042
     promoter        6262..6320
                     /label=CP25
     RBS             6331..6338
     misc_feature    6347..7063
                     /label=E2-crimson
     terminator      7068..7150
                     /label=rpoC
     primer_bind     complement(7160..7199)
                     /label=AC_041
     primer_bind     7180..7197
                     /label=AC_036
                     /note="FZ-01"
     terminator      7191..7287
                     /label=AmpR Terminator
                     /label=AmpR\Terminator
     CDS             complement(7285..8145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(7288..8145)
                     /label=AmpR
     primer_bind     complement(7355..7375)
                     /label=AC_08
     promoter        complement(8146..8276)
                     /label=AmpR Promoter
                     /label=AmpR\Promoter
     promoter        complement(8146..8250)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(8187..8215)
                     /label=amp prom
                     /label=amp\prom
     primer_bind     complement(8278..8300)
                     /label=AC_010
     primer_bind     8278..8300
                     /label=AC_18
ORIGIN
        1 ctcaaggcag ccagaacatc caaaaatccg ctgtaggcga ttctgatggc ttctgttcgg
       61 ggtatgaggc gatgtgctcc acaagcacga ccgacaagcc agccgaaacg ctcgccaagc
      121 tggccgctgc gctacctgcc ggagaggcca aacgctaccg ggtgatggtg tccattgtcc
      181 cggtggaaga atgaaggcga gcggggcggg gcggggtgtg tctggccggg cttgtcccgg
      241 ccttgtcttt tggggttttt ggtaaggact tttgacgccc tcaagggcgg cgaaaagtac
      301 cgcgcctagt ggatgatctt ggatagatca tggagggatt ttgcgaatcg cccgcaaacc
      361 cgcgccgttg ctaggttttt cagatttcgg cggggaacgt tacgccgggg gttggggaac
      421 gttacgccgg gggttgggga acgttacgcc gggggttggg cctgctgatt tcccacttgc
      481 ccttggcgta ctcgttcacc gtccaaccaa cggcggcaag ctcgacaagc gccttgcggg
      541 cggtctggcg gcgctttttc attgcttcat cgttggctgc gtctggccat acataaccgc
      601 acagcgtgtc gatttcagct ttgccagact ttccggggtc aatccagcca catagccgct
      661 ggtgtagcag ccgtgccggg tctgtttcca gcttgcgaac ttctgccatg ttgatgcggg
      721 tgtgcgggcg ctctcccata accgcatccg ccagccgggg attaagcgca acaaataact
      781 tgccttcttg ctcgtcgctc gcgtactcgg acagaatgcg gaacccttgc cgcttaccac
      841 ctctttcgac aatcaccgat accgcccaaa gccgttcgat gctttcccgg atggttttaa
      901 actgacttcc tccatcttcg gcgtagccaa gttctcgggc caactggcgg aagctgccct
      961 tggctaccat cgtatctttc tctatggcgt cccaccgcat atcaagccat aggcggagtt
     1021 gctggcctgc ttctgattcc gtttcgtgcc gtagcacgat gccgcgcccg ttatctccgg
     1081 aaattgcagc cattgccacc agcccttgca atacgcgcaa gtcatcgccg ccaagtggtt
     1141 caggccccca aaaacggatt gagtcatctc cgtagttgta ggtcacatcg agcttgagcc
     1201 tctttcgttc tccgcgcttg agactgcgga aaagccccgg cgtgaggcaa tgcgcggggt
     1261 cgtgccgcgc atgggtgagg tcatagctca ttgcgctttc cccctttttt tcctgctcca
     1321 ttgctgattg cctcctgtag ttcaacgggc aacaacaccc cgccgctgtg ccgctgatac
     1381 cagcggtcta gcggggtcgc gtcgtagttc tgcttgctca cgccaaagcg gacaaaaagg
     1441 ccgcgccgct cgttgccgat gggcgaacga tcaaagcccc ggtcactcat gcgctcggct
     1501 tcctgctccg tcattttggc gacatagccg caccatctgg cgttgtcgat cagggcagac
     1561 gcgccccgcg ctgcctgctg ctggtcggtc tggccgtcgc gggcgctgcc cttgttgacg
     1621 tggtgcaggt acaggacagc cgccccggtg gtcgccgcga tgtgttccaa cacggaaaca
     1681 aggtgggcca tgtcgccatt gctgttctcg tcgaggctgt gaatccggct cagggtgtcc
     1741 agcacaatca gccgggcccc tgcgctgtag tcgatgacgc ggcgcaagtg cgcctcgtcc
     1801 atcacgttta gccgcttgcc catgatcggc tcaagcatca ggttctcagc gatggcttcg
     1861 cgggccgact ggttgaggtg ctggccgatg gcgtggacac gtcgcacaag ggcgggctgt
     1921 ggatcttcgc cagcgagata aaccacgcgc ccggtgtgcg ccggggttag tcccacaagg
     1981 tcgccgcctg ccacactgca agcgattgac atggccgctt caagagcaaa aaagctcttg
     2041 ccagttgcgc caggtgcgac aagtgcgccc acggttccgg ccaagaatcc gggccagatg
     2101 aaatcaagtt ctggcggctc attggtgaag gccgccataa tgtctaaagc catgcttgtc
     2161 tccttttcag acagttgtgg ttaggcgctg ccggggccgc aatcccctgc cgcgccgctc
     2221 aattttcgcc cctcgatgcc agccacgccg aagcctcggc caaggcgtcc tcataggcgt
     2281 agctggccgc ttcttctgcg ttctctgctg tggcgtactc ggacaacggg aacactccgc
     2341 cgccgacttc ctcgccgctt tccaacaggc gcaaccgcca gccgccgcca aggtcggagg
     2401 ggcgcgggtc gatctggtag ctgtattcga tcattggcgg taaacctcct tacgattgcc
     2461 gatgcgcata acgaggatgc gcaaagcacc gtcctcgatg ctggcgatga ttcgccaatc
     2521 cccaacgcgg tatttccaga aggctcccag tttggagcct ttgagggctt cgccaatgct
     2581 gcgcgggtcg tcgagctggg cgacacggcc atgcaaaaaa gcgaggatgc ggcgggcggt
     2641 ctgctggtcg atcttgccta gctcgcgctc ggcggctggg tcgagttcaa ccttccatgc
     2701 catagcgttt catcacttct tcgagtggca cggtttgggt tttgcctgcg cgaatgtcga
     2761 tcaggcgttg ctctgcaagg tacaaatctt cgaggtcatc caagtgctca aggatggctt
     2821 cgcgggcata gaaagtcttg gtccgccctg tggcctgcgc cagtgcttca aggcgggttt
     2881 ccacttcggc gggcagtcgg attgcaagca tgtttcggct cctgttctga aaacgctata
     2941 caagtatatc acaaccacca cgcaacgcta gagactcatt cccggcgagt tgtcgcgctc
     3001 gatcttctgc gcctttgcct gctcctgctg tcgtacaggc tcgcgccctt ccagcttctc
     3061 ccactgctgc cggtacttgc caagatcggc ggcttgccgg tcgcctgcgg cgctgtaggc
     3121 atagcgggcg gtgcgctgcg cgtagtcgtt ccaatcgcgg ccctcgtctt tctgccgggt
     3181 ggccggtgcg cactggcgga tagcgccctc gatggccgct tgatcgtggc cggtgacgcg
     3241 catacgcacg gcaatcatgg aatccacgcg ggacaagtcc acctcgccgc cacgctgccg
     3301 cttgagcacg tcgcggtaat gccgctggta ggcgtcgatc gcgctgccgc tggccgctgc
     3361 aagctccaag gcgggcttgg ctttgctgcg ctcgggctgc tgcgccttca aggccgcttg
     3421 ccgctgatac tcggcgtcga tctggctgga caaggccagc gccttgacgc actcccggcg
     3481 ctcggccttg agcaagcgca cctctggata gctgccgtcc tcccgctggt gcttgggctt
     3541 gcggttctcg tagccgggtg cgcggtgcgg gtggatggct cccgatagct tcgggtcgcc
     3601 gtattcacgg ttcagggcat cgctcaggcg gttgcccacg tccttatcgt gggccgtccc
     3661 cagcttcgac acggtgatga tggcctgata gttgccgggg ctggattcca gcacggcggc
     3721 gggctggtag ccgtctttga taagccgctc cagcttctcc cggttcatgt cgtcgatgag
     3781 gatgtgatgc ttcttgtccg atagcggcgt gtagtagagg ttttcgcccc ggcgctgtag
     3841 gcgctgcatc tccggcgtgc gctgctcgat ctcctgcggt gtgaaacccc gcgtgatgcc
     3901 gtccttcttg tcgaggatga aggtttgctt ccttccgtct gcctgcatct tgatggacgt
     3961 tacccggtag cgctccgcgc ctaccgcttc cgcgtaccgc tcgaactgct ttagctgctc
     4021 cgatttcatc gcctgcgccc tctcctgctg tatccgctgc cgctcctgct ggatgctctc
     4081 ctgaagctct gcatttgtga tcttgaagcc gtgctctgct gctaacttcc cgcacatggc
     4141 cttgtattcg tcgttcccgg ttacggtgaa gctgccccac ttctgcgccg atagctgcaa
     4201 ggcggcaagc gtgctgtccc ggttgcgcca gtcgtgaata tcgatgcttt tgcccttgtc
     4261 cacgaaagac acacccccgc cccgcccccc ttcttccttc cggctgtaat gcacctgctg
     4321 gcccacgatt tcaggctgat aggcgcgaat gtcgcgcggc gtcggcggct cgctgcggtc
     4381 gccctcgatg cgctgcggct cgctggcccg gtgtcgccac tgctcggcaa gctccgggct
     4441 tttctgcatc cgctgccact gctccaggtc gggatagggt ctgaactgct gccggtgctg
     4501 ctcgcgctgc ttctggtgct tctccttcaa ggcggctttc tctgcggcct gctcggcggc
     4561 aatcacgctg cgcatggcgt tgagcacttc gcccttgcct ttccagttgc cgcgcatgag
     4621 ttcatcgcgg cgggccttct gctgctcggc aagttgcttg cgctcctgct cctgccgctt
     4681 gtcctgtacc agcttgtccg cgttcttctc cgagtagtgc gccttgcgcc cggtgatgta
     4741 gtccttccat cccggcacgt ctggcttgat cggctccggc tcgcgctgtg ctacctgctg
     4801 acgctgcggc ggctggtaag ctccgagtcg cttttgcaac ttggataggc tcgcgtcccg
     4861 gtctgcgctg ctggccttca cgccaacatc accgacaaag agcgtcgccc cgctgcctgt
     4921 tttctcgtag cgcatcccct tggctgctaa ctcgcggtgc aactgctccc acgtctgcgc
     4981 ctttttgatg atcggtgcgc cgtcctcaat ggcgatgcgt tcggcggatt tctccccggt
     5041 gcggttttcc atgtcgcgct tcggctgggc tggctggcgc ggcttgtcct tgtcgatgtg
     5101 ctcccggcct aactcaccat tctccaacac ctgataacgc ccgttctgct cacgctgcca
     5161 gccttgtgcg ttctcgattc gtgctatcgc cttgtgcgct gcctcaatgt cgaagccgtt
     5221 attgatcttc accaccttga gcgtttcagg atgcacgcgg ttgatggcaa ggtgcaggtg
     5281 caagttgtcc gtgtcggcgt gcaaaccata gatggcttga tggtctttaa cacctagctc
     5341 atccatgaaa atgcttaccg cttcttcgac ctgctccggg ctgggctgct cgccttctcg
     5401 ccaactcagg acatagtgat tgatcgtatc cttgcttcgc actgcctctt gtgacaaggc
     5461 aatcatttct gccgtctggc tctgtggttc atcggtgatg aatccacgcg ccccggcatg
     5521 gatgcacttc tcctgcgagt tctcacgctc aggctcacgg atgtagccag ttaggcggga
     5581 aacgcgaacc gccttgcttg ctgccttctg cgggttctta accttcttga cgatcacggc
     5641 tcagtttccc gatataggct ttcaagacca caagcgcccc ggctgtgtcc ttgctgtaca
     5701 cgcctccgct ctcgttgtgg atgtgcttaa gcaatccacc gatgcggcgc aactctttga
     5761 gcattaccgc gtcggcgctg gcaatgatcg gcctgccaaa gtagcggcgg cggacaagct
     5821 ccgacatgct caacccggca aggtcggcat cctctttcaa gcgggccttc tccgctgcgg
     5881 tcagccgtac attgataacg gcgtccagcg gttccggccc ctgcgtttca aatggcatcg
     5941 tgtctccttc tcgcgggggg cgggcggggt gtgtcttgga cagtaagaag cgagagcgcc
     6001 gcactgtccg gggtcaaggg gatacccctt gcaggataag ccagaccgtg agaagcgaag
     6061 cgccgaatgg gcaggcgtcc gttggaacca cgtggcgaag ccatgtgtgt tacaacggtc
     6121 atcctgtatt gctcaaccgc tctactatca tatcagcagt ttaggcacac tagatgcact
     6181 ttgcaacaag cataaaaggc gctatgctgt caactccgaa accagatctg aatcatgcgc
     6241 ggatgacacg aactcacgac gctttggcag tttattcttg acatgtagtg agggggctgg
     6301 tataatcaca tagtactgtt atacagaaac agaggagata ttacatatgg atagcactga
     6361 gaacgtcatc aagcccttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     6421 cgagttcgag atcgagggcg tgggcgaggg caagccctac gagggcaccc agaccgccaa
     6481 gctgcaagtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     6541 cttctacggc tccaaggcgt acatcaagca ccccgccgac atccccgact acctcaagca
     6601 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     6661 gaccgtgacc caggactcct ccctgcagga cggcaccctc atctaccacg tgaagttcat
     6721 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactctgg gctgggagcc
     6781 ctccactgag cgcaactacc cccgcgacgg cgtgctgaag ggcgagaacc acatggcgct
     6841 gaagctgaag ggcggcggcc actacctgtg tgagttcaag tccatctaca tggccaagaa
     6901 gcccgtgaag ctgcccggct accactacgt ggactacaag ctcgacatca cctcccacaa
     6961 cgaggactac accgtggtgg agcagtacga gcgcgccgag gcccgccacc acctgttcca
     7021 gactcacggt atggacgaat tgtacaagca cgacgaattg taaatccgta atcgttaatc
     7081 cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga
     7141 gcatttgtca gctggatcag cagatgggaa ccggatactc gagcggccgc gattatcaaa
     7201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7261 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7321 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7381 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     7441 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7501 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7561 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7621 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7681 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7741 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7801 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7861 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7921 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7981 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8041 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8101 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8161 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat
     8281 gaccaaaatc ccttaacgtg
//
