LOCUS       X651_pUC19-piEB1        9016 bp    DNA     circular     24-JAN-2023
FEATURES             Location/Qualifiers
     misc_feature    6629..6651
                     /dnas_title="m13 f"
                     /note="m13 f"
     misc_feature    185..207
                     /dnas_title="m13 rev"
                     /note="m13 rev"
     misc_feature    285..1713
                     /dnas_title="Untitled"
     misc_feature    285..305
                     /dnas_title="Untitled"
     misc_feature    1714..1743
                     /dnas_title="opto-EB1N 3'"
                     /note="opto-EB1N 3'"
     misc_feature    1717..1739
                     /dnas_title="guide 6"
                     /note="guide 6"
     misc_feature    1720..1742
                     /dnas_title="guide 12"
                     /note="guide 12"
     misc_feature    1722..1743
                     /dnas_title="crispr resistant sequence"
                     /note="crispr resistant sequence"
     misc_feature    4640..6618
                     /dnas_title="Untitled"
     misc_feature    6067..6087
                     /dnas_title="Untitled"
     misc_feature    6598..6618
                     /dnas_title="Untitled"
     misc_feature    1744..1836
                     /dnas_title="GCN4 Leucine Zipper"
                     /note="GCN4 Leucine Zipper"
     misc_feature    1894..2325
                     /dnas_title="LOV2 wt"
                     /note="LOV2 wt"
     misc_feature    2344..2475
                     /dnas_title="sv40polyA"
                     /note="sv40polyA"
     misc_feature    2498..2513
                     /dnas_title="start ef1a"
                     /note="start ef1a"
     misc_feature    2498..3675
                     /dnas_title="EF1alpha-promoter"
                     /note="EF1alpha-promoter"
     misc_feature    3698..4405
                     /dnas_title="mCherry"
                     /note="mCherry"
     misc_feature    4418..4594
                     /dnas_title="Zdk"
                     /note="Zdk"
     misc_feature    4448..4453
                     /dnas_title="Untitled"
     misc_feature    1663..1689
                     /dnas_title="pcr primer genomic dna analysis"
                     /note="pcr primer genomic dna analysis"
     misc_feature    4694..4720
                     /dnas_title="pcr primer genomic dna analysis"
                     /note="pcr primer genomic dna analysis"
     misc_feature    4530..4554
                     /dnas_title="forward 2 genomic analysis insertion"
                     /note="forward 2 genomic analysis insertion site"
     misc_feature    1891..1915
                     /dnas_title="reverse 1 primer genomic analysis"
                     /note="reverse 1 primer genomic analysis insertion site"
     source          1..9016
                     /dnas_title="X651_pUC19-piEB1-mCherry-knock-in, HDR
                     plasmid, EF1alphapromoter.dna"
ORIGIN      
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgGCATGC CTATACCTCA
      301 TTTGATTCAG CAGTTCATGG TCTTTTGCGT TGTTTCCCAC TTTATGGCAG ATAGGAATAA
      361 TGCTGCTGTG AACGTTCATA TACGGCTTCT GTGTGGACCT ACAAGTTTTG ATTTCTCTCA
      421 GTTACAGACC CAAGAGTATA TACTCTGGGA GCAGAATTGC TAATTGCTGT GCCATGTGTT
      481 AACTCTGTAT TTAACCTTTT GCAGAACTAC CTGTTTTCCA AAGTGCCTCA GCTCTTTACA
      541 TTCCCATCAG CAGGGTTCCC GTTTCTCTAG ATATTGAGTA CTTTTTATTC TTTACCTGTT
      601 TAGAATGGGA AACTACATAG ATAACCCCGT GCATTTCATA TTTCAGATGC TGCTACCAGT
      661 TGAGTGGAGA CGTGCATTAA GCATTTAGTC TTTGGGATAA ACGTGCTAGA GAGAGTCATC
      721 CAGAGGAAAT GCCTATTACT GTGGGTGACA GGCCTTGTGA TTATAGTTTG GGGTGTTAGG
      781 AAGATGAGAT ATTTTCAAAC CTGGCATGTA ATATGCAAAC TTTACAAAAT AATTGCTAAG
      841 TAGGTGAATC ATCAGTGATA TATATACCTT GAGGTTTAGC CCATTCTTGA TTAATTTTAC
      901 TTGTAGATAG GTTGGCTCCT TTACTCCTTT TTTCTTGTCT TTGTCTGCTT TTGGCTGTAA
      961 ATACTGAGAG ACGTCTATAA ATACTAGTCA GTGTGATGCT TTTCTCTCCT CACTCTTCTA
     1021 AACTCCTTCT AAAAGGTAAG CAGAGATGAA GATTTTAAAG CAATTCATGG GAAGAGCAGG
     1081 CTCTCCTGCT CTTATGGATT CCCTCCCTCT TCCACTCCAA GGAGTCTGAG GTGAGGAGTT
     1141 AGGAGGGACC TAACTTTCCA GATGGGGAGC AGTGGGCACC TGGCAACAGC CGAGACTGTG
     1201 TGCTCATGTC TAGCGTGAAG TTTGCCTTTG CTCTACGAAG CTGGATTTGG TTTGTAGATG
     1261 TGTTAACTAA ATCTTAGCTG TTGTAACCAA CATGAAACAA CCTGTTTGTG GATTTGGTCA
     1321 GAGCTGGGTT AAGGTGGATC ATCTCTGTGC ACCAGAATGA CTGTACCCCT TTCAGATGGT
     1381 AGTCCTGTTC ACCGCAGTGG AGAAAAGACC TCACTGAAGG ACTTATTCCC AGCATGAAAC
     1441 ATCATGACAG AAATTGGTAC TTAACTCTGC TGCCAGGATA GAAACCACTC AGTAGGCAAA
     1501 TGTGGGAGCG TTGTCTTGCA TGCGGGGGCT CTCTAGCATT GGTTCCCTTC ACTGAACACC
     1561 TGTGCAAGGC AGATGGTCAC AGCTTCTCCA TGTTGTCTTG TGGATTTTTT GTTTGTTTGG
     1621 GCTGGAATTA CTCCTTTTCA AAAACCTGTG CTCTCTTTTT CAGCTCCCCA GAGGCCCATC
     1681 TCAACACAGA GAACCGCTGC GGCTCCTAAG GCTGGCCCTG GcGTcGTtCG cAAGAAtCCa
     1741 GGTATGAAAC AGCTGGAAGA TAAAGTGGAA GAGCTGTTGA GCAAAAACTA TCATCTGGAA
     1801 AACGAAGTGG CGCGTCTGAA AAAGCTGGTG GGCGAACTCG AGCTCAAGCT TCGAATTCTG
     1861 CAGTCGACGG TACCGCGGGG TTCTGGAAGT GGATTGGCTG CTGCACTTGA ACGTATTGAG
     1921 AAGAACTTTG TCATTACTGA CCCAAGATTG CCAGATAATC CCATTATATT CGCGTCCGAT
     1981 AGTTTCTTGC AGTTGACAGA ATATAGCCGT GAAGAAATTT TGGGAAGAAA CTGCAGGTTT
     2041 CTACAAGGTC CTGAAACTGA TCGCGCGACA GTGAGAAAAA TTAGAGATGC CATAGATAAC
     2101 CAAACAGAGG TCACTGTTCA GCTGATTAAT TATACAAAGA GTGGTAAAAA GTTCTGGAAC
     2161 CTCTTTCACT TGCAGCCTAT GCGAGATCAG AAGGGAGATG TCCAGTACTT TATTGGGGTT
     2221 CAGTTGGATG GAACTGAGCA TGTCCGAGAT GCTGCCGAGA GAGAGGGAGT CATGCTGATT
     2281 AAGAAAACTG CAGAAAATAT TGATGAGGCG GCAAAAGAAC TTTAAGGATC CGCACAATTC
     2341 GAGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     2401 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     2461 TCTTATCATG TCTGGGGGAC AGCAGAGATC CAGTTTGGGC TCCGGTGCCC GTCAGTGGGC
     2521 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGATCCGG
     2581 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT
     2641 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT
     2701 TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG
     2761 CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACTGGCT GCAGTACGTG
     2821 ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC TTGCGCTTAA
     2881 GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG CCGCCGCGTG
     2941 CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT AGCCATTTAA
     3001 AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT AAATGCGGGC
     3061 CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG GGCCCGTGCG
     3121 TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG AATCGGACGG
     3181 GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC GTGTATCGCC
     3241 CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA AAGATGGCCG
     3301 CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGGAGGACGC GGCGCTCGGG AGAGCGGGCG
     3361 GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC TTCATGTGAC
     3421 TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT TTGGAGTACG
     3481 TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG
     3541 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT
     3601 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA
     3661 TTTCAGGTGT CGTGAGCTAG CGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGGA
     3721 TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA
     3781 CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC
     3841 CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC
     3901 TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT
     3961 GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG
     4021 CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA
     4081 GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCTGTAATG CAGAAGAAGA CTATGGGCTG
     4141 GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA
     4201 GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC
     4261 CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC
     4321 CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC
     4381 CGGCGGCATG GACGAGCTGT ACAAGGGTTC TGGATCCATG GTGGATAACA AATTCAATAA
     4441 AGAAAAGACG CGTGCCGGTG CGGAAATCCA TTCTCTGCCA AACCTGAATG TTGAGCAGAA
     4501 GTTTGCCTTC ATCGTGAGCC TGTTTGATGA TCCATCTCAG AGCGCCAATC TGCTGGCCGA
     4561 AGCCAAAAAA CTGAACGATG CCCAGGCCCC AAAAGGTGGT TCTGGTGGTA GCGTGGGCAA
     4621 CGGAGACGAt GAaGCAGCTG AGTTGATGCA GCAGGTGGGC ACCCCTGTGT TTAGCACGTG
     4681 AGTCACCTCG GGGGATAGGA TCCTTGCGGT GGCCAGGGTG CCTTATCCGT GTTCTTAAAT
     4741 GAACACCTGC CTTGTTTGCT TGCCAGAGCA TGTGACACGT GTGAGCCTCA GGTGCTGTGC
     4801 GGGGAGCATG AACGTGGAGA TGTAAGAGAG AGATCAGGTG TGCCTGGGCT CTCTTAGTGT
     4861 GGCAGAAGCC TGTGCTCGGA AGCCAGTGCT TAGACTAGAA TCCTGTTGAA TGTTTCTGGA
     4921 ATGCTGCCAG GGACCTGGGA TTTGGAGAAT GAAAGATAAA GGAGGTCTCC TTTTTGAATA
     4981 ATTTAATAAA AAGGATTCCG CAGTCTCTCT GCTGAGTCCT AGACTGGGGT CACTGGAGAT
     5041 TGGAATTCAG AGTGGAACTG GTATGAAAAG CCTGTGGCTC TTCTAGTTCT GTTCTTAGTA
     5101 ATTGGGTGAT TCGAAATGTC TTCTTCAGGA GTAAATGTCC TTAAGGCACC TGTCAGAGGT
     5161 CTCTGTGGAT CAAAACAAGT TTTAGACTTT TAGACATTTT CCCACTAATC CTTGTAATTG
     5221 GTTGGTTTGC TTGCTTTCAG ATGATTCTTT TTGTTAATTC TATTTTTTAA ATTTTGTTTT
     5281 ATTAAAAAAG TTTTTTTAAT AAAATAGAGA TAGGGTCTCA CTATGGTGCC AAGGCTGGTC
     5341 TCTAACTCCT GAGCTCAAGT GATCCCCCTG CCTGAGCCTC CCAAAGTGCT GGAGTTACAG
     5401 GTGTGAGTCA CCACGCCTGG CTCCATTGAA AAAATCTGGG ATGCTCTGTG ATTCCAGTGA
     5461 GTTTGGGATT GCATCCTTTT ACTTTTCTAA AAGCCAAGTC CTGAAACTTG AGGGAATTGA
     5521 TTTCTAATGC AGAACCTCTC ATGACCCACA CTCAGACTTC TAAGAGATCA GGACAGTGAG
     5581 AACCTGCCCA AAAATCACAT TTCACTTTTT TATTTTTTAT TTTCTTTTTT TGAGACGGAG
     5641 TCTTGCTGTG TCGCCCAGGC TGGAGTGCAG TGGCGTGATC TCAGCTCACT GCAACCTCCG
     5701 CCTCCCAGGT TCAAGGGATT CTTCCGCCTC AGCCTCCCGA GTAGCTGGGA CTACAGTCGT
     5761 GCGCCACCAC GCCTGGCTGA TTTTTGTATT TTTAGTAGAG ACAGGGTTTC ACCATATTGG
     5821 CCAGGCTGGT CTCCAACTCC TGACCTCAGA TGATCCACCT GCCTTGGCCT CCCAGTGCTG
     5881 GGATTACAGG CATGAACCAC TGCGCCTGGC CCACATTTCA CTTTTTCGTT CTTTCATTTG
     5941 TGTGTGCCCC GTGGTCTTAG GTGCCTTGGG AGTGTCCAGG TGACTGGGTC ACCGTCCTCA
     6001 TCCTTGGAGT GGGTGTGGCT GACTTGAGCA CCCGCTGGGG CTGTTACTTT GAAGTACAGA
     6061 AGCCTCGGAG AACAAGGAGA GACTTCCTGT AACTATTTCA AGACATGGAT TTCAAACACA
     6121 GGAAGCAGTT TCAGAAAGAA ATACGAGAGA GTAAAGAGTG GTTAATGTTG AGGCTGGGTA
     6181 GCAGCGACTT GATCTGTGCT CTCCGAGAGA GCGTGCCAGG TTCTGCAGTG TGGCTGCTCT
     6241 CTGCTCAGCT CTTTTTTTTT TTTAATTATT ATTATTATTA TACTTTAAGT TTTAGGGTAC
     6301 ATGTGTACAA CGTGCAGGTT AGTTACATAT GTATACATGT GCCATGCTGG TGCACTGCAC
     6361 CCACTAACTC GTCATCTAGC ATTAGGTATA TCTCCCAATG CTATCCCTCC CCCCTCCCCC
     6421 CACCGCACAA CAGTCCCCAG AGTGTGATGT TCCCCTTCCT GTGTCCATGT GTTCTCATCC
     6481 GCTCAGCTCT TATCCTGGGT GATGGAGCCC AGGCTTGGGG TTCCATAGAT TGAGGGAGAA
     6541 GTAGGAGTGT CTCTGTCAGT TAACTTCTAT CTGGACAAGT CAGCCTCTCT GAACTTACTG
     6601 CCTCACGTGT GAAGTGGGca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6661 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6721 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     6781 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     6841 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6901 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6961 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7021 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7081 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7141 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7201 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7261 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7321 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7381 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7441 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7501 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7621 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7741 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7801 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7981 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8041 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8161 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8221 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8281 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8341 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8401 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8461 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8521 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8581 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8641 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8701 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8761 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8821 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8881 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8941 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9001 gcgaggaagc ggaaga
//

