LOCUS       X718_pUC19-piEB1        9025 bp    DNA     circular     24-JAN-2023
FEATURES             Location/Qualifiers
     misc_feature    6638..6660
                     /dnas_title="m13 f"
                     /note="m13 f"
     misc_feature    185..207
                     /dnas_title="m13 rev"
                     /note="m13 rev"
     misc_feature    285..1713
                     /dnas_title="Untitled"
     misc_feature    285..305
                     /dnas_title="Untitled"
     misc_feature    1714..1743
                     /dnas_title="opto-EB1N 3'"
                     /note="opto-EB1N 3'"
     misc_feature    1717..1739
                     /dnas_title="guide 6"
                     /note="guide 6"
     misc_feature    1720..1742
                     /dnas_title="guide 12"
                     /note="guide 12"
     misc_feature    1722..1743
                     /dnas_title="crispr resistant sequence"
                     /note="crispr resistant sequence"
     misc_feature    4649..6627
                     /dnas_title="Untitled"
     misc_feature    6076..6096
                     /dnas_title="Untitled"
     misc_feature    6607..6627
                     /dnas_title="Untitled"
     misc_feature    1744..1836
                     /dnas_title="GCN4 Leucine Zipper"
                     /note="GCN4 Leucine Zipper"
     misc_feature    1894..2325
                     /dnas_title="LOV2 wt"
                     /note="LOV2 wt"
     misc_feature    2344..2475
                     /dnas_title="sv40polyA"
                     /note="sv40polyA"
     misc_feature    2498..2513
                     /dnas_title="start ef1a"
                     /note="start ef1a"
     misc_feature    2498..3675
                     /dnas_title="EF1alpha-promoter"
                     /note="EF1alpha-promoter"
     misc_feature    4427..4603
                     /dnas_title="Zdk"
                     /note="Zdk"
     misc_feature    4457..4462
                     /dnas_title="Untitled"
     misc_feature    1663..1689
                     /dnas_title="pcr primer genomic dna analysis"
                     /note="pcr primer genomic dna analysis"
     misc_feature    4703..4729
                     /dnas_title="pcr primer genomic dna analysis"
                     /note="pcr primer genomic dna analysis"
     misc_feature    4539..4563
                     /dnas_title="forward 2 genomic analysis insertion"
                     /note="forward 2 genomic analysis insertion site"
     misc_feature    1891..1915
                     /dnas_title="reverse 1 primer genomic analysis"
                     /note="reverse 1 primer genomic analysis insertion site"
     misc_feature    3698..4414
                     /dnas_title="EGFP"
                     /note="EGFP"
     misc_feature    3666..3725
                     /dnas_title="#480 GA forward"
     misc_feature    4392..4451
                     /dnas_title="#481 gibson reverse"
     source          1..9025
                     /dnas_title="X718_pUC19-piEB1-EGFP-knock-in, HDR plasmid,
                     EF1alphapromoter.dna"
ORIGIN      
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgGCATGC CTATACCTCA
      301 TTTGATTCAG CAGTTCATGG TCTTTTGCGT TGTTTCCCAC TTTATGGCAG ATAGGAATAA
      361 TGCTGCTGTG AACGTTCATA TACGGCTTCT GTGTGGACCT ACAAGTTTTG ATTTCTCTCA
      421 GTTACAGACC CAAGAGTATA TACTCTGGGA GCAGAATTGC TAATTGCTGT GCCATGTGTT
      481 AACTCTGTAT TTAACCTTTT GCAGAACTAC CTGTTTTCCA AAGTGCCTCA GCTCTTTACA
      541 TTCCCATCAG CAGGGTTCCC GTTTCTCTAG ATATTGAGTA CTTTTTATTC TTTACCTGTT
      601 TAGAATGGGA AACTACATAG ATAACCCCGT GCATTTCATA TTTCAGATGC TGCTACCAGT
      661 TGAGTGGAGA CGTGCATTAA GCATTTAGTC TTTGGGATAA ACGTGCTAGA GAGAGTCATC
      721 CAGAGGAAAT GCCTATTACT GTGGGTGACA GGCCTTGTGA TTATAGTTTG GGGTGTTAGG
      781 AAGATGAGAT ATTTTCAAAC CTGGCATGTA ATATGCAAAC TTTACAAAAT AATTGCTAAG
      841 TAGGTGAATC ATCAGTGATA TATATACCTT GAGGTTTAGC CCATTCTTGA TTAATTTTAC
      901 TTGTAGATAG GTTGGCTCCT TTACTCCTTT TTTCTTGTCT TTGTCTGCTT TTGGCTGTAA
      961 ATACTGAGAG ACGTCTATAA ATACTAGTCA GTGTGATGCT TTTCTCTCCT CACTCTTCTA
     1021 AACTCCTTCT AAAAGGTAAG CAGAGATGAA GATTTTAAAG CAATTCATGG GAAGAGCAGG
     1081 CTCTCCTGCT CTTATGGATT CCCTCCCTCT TCCACTCCAA GGAGTCTGAG GTGAGGAGTT
     1141 AGGAGGGACC TAACTTTCCA GATGGGGAGC AGTGGGCACC TGGCAACAGC CGAGACTGTG
     1201 TGCTCATGTC TAGCGTGAAG TTTGCCTTTG CTCTACGAAG CTGGATTTGG TTTGTAGATG
     1261 TGTTAACTAA ATCTTAGCTG TTGTAACCAA CATGAAACAA CCTGTTTGTG GATTTGGTCA
     1321 GAGCTGGGTT AAGGTGGATC ATCTCTGTGC ACCAGAATGA CTGTACCCCT TTCAGATGGT
     1381 AGTCCTGTTC ACCGCAGTGG AGAAAAGACC TCACTGAAGG ACTTATTCCC AGCATGAAAC
     1441 ATCATGACAG AAATTGGTAC TTAACTCTGC TGCCAGGATA GAAACCACTC AGTAGGCAAA
     1501 TGTGGGAGCG TTGTCTTGCA TGCGGGGGCT CTCTAGCATT GGTTCCCTTC ACTGAACACC
     1561 TGTGCAAGGC AGATGGTCAC AGCTTCTCCA TGTTGTCTTG TGGATTTTTT GTTTGTTTGG
     1621 GCTGGAATTA CTCCTTTTCA AAAACCTGTG CTCTCTTTTT CAGCTCCCCA GAGGCCCATC
     1681 TCAACACAGA GAACCGCTGC GGCTCCTAAG GCTGGCCCTG GcGTcGTtCG cAAGAAtCCa
     1741 GGTATGAAAC AGCTGGAAGA TAAAGTGGAA GAGCTGTTGA GCAAAAACTA TCATCTGGAA
     1801 AACGAAGTGG CGCGTCTGAA AAAGCTGGTG GGCGAACTCG AGCTCAAGCT TCGAATTCTG
     1861 CAGTCGACGG TACCGCGGGG TTCTGGAAGT GGATTGGCTG CTGCACTTGA ACGTATTGAG
     1921 AAGAACTTTG TCATTACTGA CCCAAGATTG CCAGATAATC CCATTATATT CGCGTCCGAT
     1981 AGTTTCTTGC AGTTGACAGA ATATAGCCGT GAAGAAATTT TGGGAAGAAA CTGCAGGTTT
     2041 CTACAAGGTC CTGAAACTGA TCGCGCGACA GTGAGAAAAA TTAGAGATGC CATAGATAAC
     2101 CAAACAGAGG TCACTGTTCA GCTGATTAAT TATACAAAGA GTGGTAAAAA GTTCTGGAAC
     2161 CTCTTTCACT TGCAGCCTAT GCGAGATCAG AAGGGAGATG TCCAGTACTT TATTGGGGTT
     2221 CAGTTGGATG GAACTGAGCA TGTCCGAGAT GCTGCCGAGA GAGAGGGAGT CATGCTGATT
     2281 AAGAAAACTG CAGAAAATAT TGATGAGGCG GCAAAAGAAC TTTAAGGATC CGCACAATTC
     2341 GAGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     2401 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     2461 TCTTATCATG TCTGGGGGAC AGCAGAGATC CAGTTTGGGC TCCGGTGCCC GTCAGTGGGC
     2521 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGATCCGG
     2581 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT
     2641 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT
     2701 TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG
     2761 CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACTGGCT GCAGTACGTG
     2821 ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC TTGCGCTTAA
     2881 GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG CCGCCGCGTG
     2941 CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT AGCCATTTAA
     3001 AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT AAATGCGGGC
     3061 CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG GGCCCGTGCG
     3121 TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG AATCGGACGG
     3181 GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC GTGTATCGCC
     3241 CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA AAGATGGCCG
     3301 CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGGAGGACGC GGCGCTCGGG AGAGCGGGCG
     3361 GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC TTCATGTGAC
     3421 TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT TTGGAGTACG
     3481 TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG
     3541 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT
     3601 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA
     3661 TTTCAGGTGT CGTGAGCTAG CGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT
     3721 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT
     3781 CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT
     3841 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG
     3901 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC
     3961 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA
     4021 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG
     4081 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG
     4141 CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT
     4201 CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC
     4261 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT
     4321 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
     4381 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGTTCT GGATCCATGG TGGATAACAA
     4441 ATTCAATAAA GAAAAGACGC GTGCCGGTGC GGAAATCCAT TCTCTGCCAA ACCTGAATGT
     4501 TGAGCAGAAG TTTGCCTTCA TCGTGAGCCT GTTTGATGAT CCATCTCAGA GCGCCAATCT
     4561 GCTGGCCGAA GCCAAAAAAC TGAACGATGC CCAGGCCCCA AAAGGTGGTT CTGGTGGTAG
     4621 CGTGGGCAAC GGAGACGAtG AaGCAGCTGA GTTGATGCAG CAGGTGGGCA CCCCTGTGTT
     4681 TAGCACGTGA GTCACCTCGG GGGATAGGAT CCTTGCGGTG GCCAGGGTGC CTTATCCGTG
     4741 TTCTTAAATG AACACCTGCC TTGTTTGCTT GCCAGAGCAT GTGACACGTG TGAGCCTCAG
     4801 GTGCTGTGCG GGGAGCATGA ACGTGGAGAT GTAAGAGAGA GATCAGGTGT GCCTGGGCTC
     4861 TCTTAGTGTG GCAGAAGCCT GTGCTCGGAA GCCAGTGCTT AGACTAGAAT CCTGTTGAAT
     4921 GTTTCTGGAA TGCTGCCAGG GACCTGGGAT TTGGAGAATG AAAGATAAAG GAGGTCTCCT
     4981 TTTTGAATAA TTTAATAAAA AGGATTCCGC AGTCTCTCTG CTGAGTCCTA GACTGGGGTC
     5041 ACTGGAGATT GGAATTCAGA GTGGAACTGG TATGAAAAGC CTGTGGCTCT TCTAGTTCTG
     5101 TTCTTAGTAA TTGGGTGATT CGAAATGTCT TCTTCAGGAG TAAATGTCCT TAAGGCACCT
     5161 GTCAGAGGTC TCTGTGGATC AAAACAAGTT TTAGACTTTT AGACATTTTC CCACTAATCC
     5221 TTGTAATTGG TTGGTTTGCT TGCTTTCAGA TGATTCTTTT TGTTAATTCT ATTTTTTAAA
     5281 TTTTGTTTTA TTAAAAAAGT TTTTTTAATA AAATAGAGAT AGGGTCTCAC TATGGTGCCA
     5341 AGGCTGGTCT CTAACTCCTG AGCTCAAGTG ATCCCCCTGC CTGAGCCTCC CAAAGTGCTG
     5401 GAGTTACAGG TGTGAGTCAC CACGCCTGGC TCCATTGAAA AAATCTGGGA TGCTCTGTGA
     5461 TTCCAGTGAG TTTGGGATTG CATCCTTTTA CTTTTCTAAA AGCCAAGTCC TGAAACTTGA
     5521 GGGAATTGAT TTCTAATGCA GAACCTCTCA TGACCCACAC TCAGACTTCT AAGAGATCAG
     5581 GACAGTGAGA ACCTGCCCAA AAATCACATT TCACTTTTTT ATTTTTTATT TTCTTTTTTT
     5641 GAGACGGAGT CTTGCTGTGT CGCCCAGGCT GGAGTGCAGT GGCGTGATCT CAGCTCACTG
     5701 CAACCTCCGC CTCCCAGGTT CAAGGGATTC TTCCGCCTCA GCCTCCCGAG TAGCTGGGAC
     5761 TACAGTCGTG CGCCACCACG CCTGGCTGAT TTTTGTATTT TTAGTAGAGA CAGGGTTTCA
     5821 CCATATTGGC CAGGCTGGTC TCCAACTCCT GACCTCAGAT GATCCACCTG CCTTGGCCTC
     5881 CCAGTGCTGG GATTACAGGC ATGAACCACT GCGCCTGGCC CACATTTCAC TTTTTCGTTC
     5941 TTTCATTTGT GTGTGCCCCG TGGTCTTAGG TGCCTTGGGA GTGTCCAGGT GACTGGGTCA
     6001 CCGTCCTCAT CCTTGGAGTG GGTGTGGCTG ACTTGAGCAC CCGCTGGGGC TGTTACTTTG
     6061 AAGTACAGAA GCCTCGGAGA ACAAGGAGAG ACTTCCTGTA ACTATTTCAA GACATGGATT
     6121 TCAAACACAG GAAGCAGTTT CAGAAAGAAA TACGAGAGAG TAAAGAGTGG TTAATGTTGA
     6181 GGCTGGGTAG CAGCGACTTG ATCTGTGCTC TCCGAGAGAG CGTGCCAGGT TCTGCAGTGT
     6241 GGCTGCTCTC TGCTCAGCTC TTTTTTTTTT TTAATTATTA TTATTATTAT ACTTTAAGTT
     6301 TTAGGGTACA TGTGTACAAC GTGCAGGTTA GTTACATATG TATACATGTG CCATGCTGGT
     6361 GCACTGCACC CACTAACTCG TCATCTAGCA TTAGGTATAT CTCCCAATGC TATCCCTCCC
     6421 CCCTCCCCCC ACCGCACAAC AGTCCCCAGA GTGTGATGTT CCCCTTCCTG TGTCCATGTG
     6481 TTCTCATCCG CTCAGCTCTT ATCCTGGGTG ATGGAGCCCA GGCTTGGGGT TCCATAGATT
     6541 GAGGGAGAAG TAGGAGTGTC TCTGTCAGTT AACTTCTATC TGGACAAGTC AGCCTCTCTG
     6601 AACTTACTGC CTCACGTGTG AAGTGGGcac tggccgtcgt tttacaacgt cgtgactggg
     6661 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6721 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6781 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     6841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     6901 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6961 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7021 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7081 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8401 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8881 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8941 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9001 agtcagtgag cgaggaagcg gaaga
//

