LOCUS       Exported                9099 bp ds-DNA     circular SYN 27-JAN-2023
DEFINITION  A:T-to-G:C base editing.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV_ABEmax_P2A_GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP,
            Raguram A, Liu DR
  TITLE     Improving cytidine and adenine base editors by expression 
            optimization and ancestral reconstruction.
  JOURNAL   Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
  PUBMED    29813047
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 1, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      132..335
                     /standard_name="CMV promoter"
                     /label=CMV promoter
                     /note="Geneious type: promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     regulatory      377..395
                     /standard_name="T7 promoter"
                     /label=T7 promoter
                     /note="Geneious type: promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    400..412
                     /standard_name="Kozak"
                     /label=Kozak
                     /note="Geneious type: motif"
     CDS             409..411
                     /standard_name="start"
                     /label=start
     CDS             412..465
                     /standard_name="BPNLS(SV40)"
                     /label=BPNLS(SV40)
     CDS             463..963
                     /standard_name="TadA*8e"
                     /label=TadA*8e
     misc_feature    529..531
                     /standard_name="W23R"
                     /label=W23R
     misc_feature    568..570
                     /standard_name="H36L"
                     /label=H36L
     misc_feature    604..606
                     /standard_name="P48A"
                     /label=P48A
     misc_feature    613..615
                     /standard_name="R51L"
                     /label=R51L
     misc_feature    712..714
                     /standard_name="L84F"
                     /label=L84F
     misc_feature    778..780
                     /standard_name="A106V"
                     /label=A106V
     misc_feature    784..786
                     /standard_name="D108N"
                     /label=D108N
     misc_feature    787..789
                     /standard_name="A109S"
                     /label=A109S
     misc_feature    793..795
                     /standard_name="T111R"
                     /label=T111R
     misc_feature    817..819
                     /standard_name="D119N"
                     /label=D119N
     misc_feature    826..828
                     /standard_name="H122N"
                     /label=H122N
     misc_feature    829..831
                     /standard_name="H123Y"
                     /label=H123Y
     misc_feature    898..900
                     /standard_name="S146C"
                     /label=S146C
     misc_feature    901..903
                     /standard_name="D147 (changed back from D147Y in ABE7.10)"
                     /label=D147 (changed back from D147Y in ABE7.10)
     misc_feature    907..909
                     /standard_name="F149Y"
                     /label=F149Y
     misc_feature    916..918
                     /standard_name="R152P"
                     /label=R152P
     misc_feature    925..927
                     /standard_name="E155V"
                     /label=E155V
     misc_feature    928..930
                     /standard_name="I156F"
                     /label=I156F
     misc_feature    931..933
                     /standard_name="K157N"
                     /label=K157N
     misc_feature    958..960
                     /standard_name="T166I"
                     /label=T166I
     misc_feature    961..963
                     /standard_name="D167N"
                     /label=D167N
     CDS             964..1059
                     /standard_name="linker"
                     /label=linker
     CDS             1057..5160
                     /standard_name="nSpCas9(D10A)-NRTH"
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=nSpCas9(D10A)-NRTH
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the D10A mutation in the RuvC catalytic domain"
     misc_difference 1084..1086
                     /standard_name="D10A"
                     /label=D10A
     misc_difference 2020..2022
                     /standard_name="I322V"
                     /label=I322V
     misc_difference 2281..2283
                     /standard_name="S409I"
                     /label=S409I
     misc_difference 2335..2337
                     /standard_name="E427G"
                     /label=E427G
     misc_difference 3016..3018
                     /standard_name="R654L"
                     /label=R654L
     misc_difference 3313..3315
                     /standard_name="R753G"
                     /label=R753G
     misc_difference 4396..4398
                     /standard_name="R1114G"
                     /label=R1114G
     misc_difference 4459..4461
                     /standard_name="D1135N"
                     /label=D1135N
     misc_difference 4594..4596
                     /standard_name="D1180G"
                     /label=D1180G
     misc_difference 4708..4710
                     /standard_name="G1218S"
                     /label=G1218S
     misc_difference 4711..4713
                     /standard_name="E1219V"
                     /label=E1219V
     misc_difference 4717..4719
                     /standard_name="Q1221H"
                     /label=Q1221H
     misc_difference 4801..4803
                     /standard_name="P1249S"
                     /label=P1249S
     misc_difference 4813..4815
                     /standard_name="E1253K"
                     /label=E1253K
     misc_difference 5017..5019
                     /standard_name="P1321S"
                     /label=P1321S
     misc_difference 5050..5052
                     /standard_name="D1332G"
                     /label=D1332G
     misc_difference 5059..5061
                     /standard_name="R1335L"
                     /label=R1335L
     CDS             5161..5184
                     /standard_name="linker"
                     /label=linker
     CDS             5185..5235
                     /standard_name="BPNLS(SV40)"
                     /label=BPNLS(SV40)
     CDS             5236..5247
                     /standard_name="linker"
                     /label=linker
     CDS             5248..5313
                     /standard_name="3xFLAG"
                     /label=3xFLAG
                     /note="pLannotate"
     CDS             5314..5322
                     /standard_name="linker"
                     /label=linker
     CDS             5323..5388
                     /standard_name="P2A"
                     /label=P2A
     CDS             5389..6105
                     /standard_name="EGFP"
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
     CDS             6106..6108
     misc_feature    6160..6384
                     /standard_name="bGH poly(A) signal"
                     /label=bGH poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6479..6495
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(6503..6533)
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="Geneious type: promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6548..6569
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6857..7445)
                     /direction=LEFT
     CDS             complement(7616..8476)
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(8477..8581)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious type: promoter"
     regulatory      join(8851..9099,1..131)
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="Geneious type: enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaacagca aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 gatttctacc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
      961 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag acaagaagta cagcatcggc
     1081 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     1141 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     1201 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     1261 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     1321 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     1381 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     1441 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     1501 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1561 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1621 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1681 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     1741 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1801 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1861 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1921 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1981 aacaccgaga tcaccaaggc ccccctgagc gcctctatgg tgaagagata cgacgagcac
     2041 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     2101 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     2161 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     2221 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     2281 atcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcagggcgat
     2341 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     2401 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     2461 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     2521 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2581 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2641 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2701 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2761 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2821 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2881 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2941 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     3001 aagcagctga agcggctgag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     3061 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     3121 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     3181 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     3241 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     3301 aaagtgatgg gcggccacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     3361 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     3421 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     3481 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3541 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     3601 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3661 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3721 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3781 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3841 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3901 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3961 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     4021 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     4081 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     4141 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     4201 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     4261 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     4321 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     4381 tctatcctgc ccaagggcaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     4441 aagaagtacg gcggcttcaa cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     4501 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     4561 atggaaagaa gcagcttcga gaagaatccc atcggctttc tggaagccaa gggctacaaa
     4621 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     4681 ggccggaaga gaatgctggc ctctgccagc gtgctgcaca agggaaacga actggccctg
     4741 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     4801 agcgaggata ataagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     4861 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     4921 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     4981 atcatccacc tgtttaccct gaccaatctg ggagccagcg ccgccttcaa gtactttgac
     5041 accaccatcg gccggaagct gtacaccagc accaaagagg tgctggacgc caccctgatc
     5101 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     5161 ggatccggcg gaggcggaag cgggaaaaga accgccgacg gcagcgaatt cgagcccaag
     5221 aagaagagga aagtctcgag cggaggcgac tacaaagacc atgacggtga ttataaagat
     5281 catgacatcg attacaagga tgacgatgac aagggaggcg gtggaagcgg cgcaacaaac
     5341 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggtgagcaag
     5401 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5461 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5521 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5581 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5641 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5701 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5761 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5821 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5881 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5941 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     6001 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6061 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgacc ggtcatcatc
     6121 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6181 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6241 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6301 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6361 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     6421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     6541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac
     7501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7741 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8581 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     8641 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8701 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8761 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     8821 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     8881 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     8941 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9001 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9061 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
