LOCUS       pEPSW0CM0032_ARF18_CDSw 3867 bp ds-DNA     circular     07-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     Terminator      complement(27..121)
                     /label="lambda t0 terminator"
     CDS             complement(142..801)
                     /label="CmR (cat, chloramphenicol acetyltransferase)"
     primer          967..989
                     /label="pUAP/DP2-F"
                     /note="sequence: ggcgtatcacgaggcagaatttc"
     misc_feature    1036..1036
                     /label="here, keep"
     misc_feature    1044..1047
                     /label="CDS1 fusion site"
     misc_feature    1045..2853
                     /label="ARF18_CDS"
     CDS             1045..2853
                     /label="Translation 1045-2853"
                     /translation="MASVEGDDDFGSSSSRSYQDQLYTELWKVCAGPLVEVPRAQERVFYFPQGHMEQLVASTNQGINSEEIPVFDLPPKILCRVLDVTLKAEHETDEVYAQITLQPEEDQSEPTSLDPPIVGPTKQEFHSFVKILTASDTSTHGGFSVLRKHATECLPSLDMTQATPTQELVTRDLHGFEWRFKHIFRGQPRRHLLTTGWSTFVSSKRLVAGDAFVFLRGENGDLRVGVRRLARHQSTMPTSVISSQSMHLGVLATASHAVRTTTIFVVFYKPRISQFIVGVNKYMEAIKHGFSLGTRFRMRFEGEESPERIFTGTIVGSGDLSSQWPASKWRSLQVQWDEPTTVQRPDKVSPWEIEPFLATSPISTPAQQPQSKCKRSRPIEPSVKTPAPPSFLYSLPQSQDSINASLKLFQDPSLERISGGYSSNNSFKPETPPPPTNCSYRLFGFDLTSNSPAPIPQDKQPMDTCGAAKCQEPITPTSMSEQKKQQTSRSRTKVQMQGIAVGRAVDLTLLKSYDELIDELEEMFEIQGQLLARDKWIVVFTDDEGDMMLAGDDPWNEFCKMAKKIFIYSSDEVKKMTTKLKISSSLENEEYGNESFENRSRG*"
     primer          complement(2820..2857)
                     /label="SW11-p32-no-stop-R"
                     /note="sequence: AAGCTCTTCgtctcAcgaaccCCCCCTACTACGATTTTCGAATGATTCATTA"
     misc_feature    2854..2857
                     /label="3'UTR fusion site"
     primer          complement(2880..2900)
                     /label="pUAP/DP2-R"
                     /note="sequence: ggtggtgacaccttgcccttt"
     Replication_ori complement(3123..3711)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 ACTTGGTCTG ACAGCTCGAG GCTTGGATTC TCACCAATAA AAAACGCCCG GCGGCAACCG
       61 AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA CTGGATCTAT CAACAGGAGT
      121 CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA
      181 TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC
      241 CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC
      301 GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT
      361 GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC
      421 GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC
      481 ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC
      541 ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGAAATTCCG GATGAGCATT
      601 CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC
      661 GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC
      721 TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA
      781 TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA
      841 AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCC
      901 GATCAACTCG AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA
      961 AAAATAGGCG TATCACGAGG CAGAATTTCA GATAAAAAAA ATCCTTAGCT TTCGCTAAGG
     1021 ATGATTTCTG GAATTCggtc tcAaatgGCG AGTGTTGAAG GTGATGATGA TTTCGGAAGT
     1081 TCTTCGTCAA GGTCTTATCA AGATCAACTA TACACAGAGC TATGGAAAGT TTGTGCAGGT
     1141 CCATTAGTGG AAGTTCCTCG TGCTCAAGAG AGAGTTTTCT ACTTCCCTCA GGGTCACATG
     1201 GAACAACTTG TGGCGTCAAC TAATCAAGGA ATCAATTCAG AAGAAATACC TGTTTTTGAT
     1261 CTTCCTCCAA AGATACTTTG TCGAGTTCTT GATGTCACTT TAAAGGCGGA GCATGAAACA
     1321 GATGAGGTTT ACGCTCAGAT CACATTACAA CCAGAGGAAG ATCAAAGTGA ACCAACAAGT
     1381 CTTGATCCAC CTATTGTTGG ACCAACTAAG CAAGAGTTTC ATTCGTTTGT TAAGATTTTA
     1441 ACGGCTTCAG ATACAAGCAC TCATGGTGGA TTCTCTGTTC TTCGTAAACA CGCCACTGAA
     1501 TGCTTGCCTT CTTTGGATAT GACACAAGCT ACTCCTACTC AAGAACTTGT GACTAGAGAT
     1561 CTTCATGGCT TTGAATGGAG GTTTAAGCAT ATATTCAGAG GACAACCACG GAGGCATTTG
     1621 CTTACTACGG GTTGGAGTAC ATTTGTATCC TCGAAAAGAC TTGTAGCTGG AGATGCTTTT
     1681 GTGTTCTTGA GGGGTGAGAA TGGGGATTTA CGGGTTGGAG TGAGACGATT AGCTCGGCAT
     1741 CAAAGCACAA TGCCTACTTC GGTTATTTCA AGTCAGAGCA TGCATTTGGG AGTTCTTGCT
     1801 ACAGCTTCTC ATGCTGTGCG TACAACAACA ATCTTTGTTG TCTTTTACAA GCCTAGGATA
     1861 AGCCAATTCA TAGTTGGGGT GAACAAGTAT ATGGAAGCTA TAAAGCATGG ATTTTCTCTC
     1921 GGTACCCGAT TCAGAATGAG GTTTGAAGGA GAAGAGTCTC CTGAGAGAAT ATTTACTGGT
     1981 ACGATTGTGG GAAGTGGAGA TCTATCTTCA CAATGGCCAG CTTCTAAATG GAGGTCATTG
     2041 CAGGTACAAT GGGATGAGCC AACAACAGTT CAaagaCCAG ATAAAGTCTC ACCATGGGAG
     2101 ATAGAGCCTT TCTTGGCAAC TTCCCCAATT TCAACTCCTG CTCAACAACC ACAATCGAAA
     2161 TGCAAGCGGT CAAGACCCAT CGAGCCATCA GTTAAAACAC CAGCCCCACC TAGTTTCTTG
     2221 TACAGCCTCC CTCAGAGCCA AGATTCCATT AATGCATCCC TTAAACTGTT TCAAGATCCA
     2281 TCACTTGAGA GAATTTCAGG TGGATACTCC TCAAACAACA GCTTCAAACC CGAGACTCCT
     2341 CCTCCTCCAA CGAATTGTAG CTATAGGTTG TTTGGATTTG ATCTCACAAG CAATTCTCCT
     2401 GCTCCAATCC CTCAAGACAA GCAACCGATG GATACTTGTG GAGCTGCCAA GTGTCAAGAA
     2461 CCCATCACTC CAACCTCAAT GAGTGAGCAG AAGAAGCAAC AAACATCAAG AAGTCGAACT
     2521 AAAGTGCAAA TGCAAGGCAT TGCGGTTGGT CGTGCGGTTG ATTTAACACT GTTGAAATCT
     2581 TACGATGAAC TGATTGATGA GCTTGAGGAG ATGTTTGAGA TTCAAGGACA GCTTCTTGCC
     2641 CGAGACAAAT GGATCGTTGT CTTCACTGAT GATGAAGGAG ATATGATGCT TGCTGGTGAT
     2701 GATCCGTGGA ATGAGTTTTG CAAGATGGCA AAGAAGATAT TTATATATTC GAGCGATGAG
     2761 GTTAAGAAAA TGACAACGAA ACTGAAGATT TCTTCGTCGT TAGAGAATGA GGAATATGGT
     2821 AATGAATCAT TCGAAAATCG TAGTAGGGGG tgagcttTga gaccCTGCAG TCCGGCAAAA
     2881 AAGGGCAAGG TGTCACCACC CTGCCCTTTT TCTTTAAAAC CGAAAAGATT ACTTCGCGTT
     2941 ATGCAGGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
     3001 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
     3061 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
     3121 TTTTTCCACA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
     3181 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
     3241 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
     3301 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
     3361 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
     3421 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     3481 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     3541 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     3601 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     3661 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     3721 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     3781 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     3841 AAATCAATCT AAAGTATATA TGAGTAA
//