LOCUS       Copy_of_pEPOZ0CM0220    2574 bp ds-DNA     circular     08-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(90..749)
                     /label="CmR (cat, chloramphenicol acetyltransferase)"
     primer          854..875
                     /label="ptoz011 pPUAP4-F"
                     /note="sequence: ACTCGAGTGCCACCTGACGTCT"
     misc_feature    984..984
                     /label="here, keep"
     misc_feature    992..995
                     /label="CDS1 fusion site"
     misc_feature    993..1429
                     /label="AT5G57420.1 Exon 1"
     CDS             993..1508
                     /label="Translation 993-1508"
     exon            1430..1508
                     /label="AT5G57420.1 Exon 2"
     misc_feature    1509..1512
                     /label="3'UTR fusion site"
     primer          complement(1591..1612)
                     /label="ptoz012 pPUAP4-R"
                     /note="sequence: AGCGAGGAAGCCTGCATAACGC"
     Replication_ori complement(1778..2366)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     Terminator      complement(2549..69)
                     /label="lambda t0 terminator"
ORIGIN
        1 GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT GGATCTATCA
       61 ACAGGAGTCC AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC
      121 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC
      181 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA
      241 ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC
      301 CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG
      361 TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG
      421 TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA
      481 GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG AAATTCCGGA
      541 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT
      601 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT
      661 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG
      721 GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT
      781 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT
      841 ACGTGCCCGA TCAACTCGAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
      901 AACCTATAAA AATAGGCGTA TCACGAGGCA GAATTTCAGA TAAAAAAAAT CCTTAGCTTT
      961 CGCTAAGGAT GATTTCTGGA ATTCggtctc AaatgAATAG TTTCGAGCCA CAAAGCCAAG
     1021 ACTCTTTGCA AAGAAGGTTT CATCAAGACA ACAGCACCAC ACAACAACCT CGTGACACCA
     1081 CGACACCTTT CATACCCAAA CCGGCTTCCa aaaaCCATAA TAATAGCAAC TCCAGCTCTG
     1141 GAGCGGCCGG GAGATCATTC CAAGGCTTTG GGCTTAACGT AGAGGACGAT CTTGTTTCAT
     1201 CGGTGGTTCC TCCGGTTACG GTTGTGCTAG AGGGACGTTC TATATGTCAA CGCATAAGCC
     1261 TAGACAAGCA TGGGAGTTAT CAGAGCTTGG CTTCGGCTCT AAGGCAAATG TTTGTCGATG
     1321 GAGCTGATTC AACGGACGAT CTTGATCTGT CAAACGCCAT TCCTGGCCAT CTTATTGCTT
     1381 ATGAAGACAT GGAGAACGAT CTCCTTCTTG CCGGAGATCT TACTTGGAAG GACTTTGTTC
     1441 GTGTAGCGAA GAGAATTCGA ATCTTGCCGG TCAAGGGAAA CACAAGACAA GTTAAAAGAA
     1501 ACGAGTGAgc ttTgagaccC TGCAGTCCGG CAAAAAAGGG CAAGGTGTCA CCACCCTGCC
     1561 CTTTTTCTTT AAAACCGAAA AGATTACTTC GCGTTATGCA GGCTTCCTCG CTCACTGACT
     1621 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
     1681 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
     1741 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCACAGGCTC CGCCCCCCTG
     1801 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     1861 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     1921 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     1981 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     2041 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     2101 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     2161 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
     2221 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     2281 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     2341 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
     2401 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
     2461 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
     2521 AAACTTGGTC TGACAGCTCG AGGCTTGGAT TCTCACCAAT AAAAAACGCC CGGC
//