LOCUS       pEPSW1KN0033            7811 bp ds-DNA     circular     01-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2..4
                     /label="TAC fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    18..42
                     /label="RB"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1104..2104)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1343..1972)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1712..2104)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2401..3474
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2698..3698
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3540..3734
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(4108..4368)
                     /label="pBR322"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4389..5003)
                     /label="rep\(pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(4508..4788)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5083..5895)
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5896..6323)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6328..6353
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6371..6373
                     /label="GCA fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Promoter        6379..6804
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     5'_UTR          6821..6882
                     /label="TMV Omega"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     Terminator      7604..7807
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 atactgaaga gcaattctga caggatatat tggcgggtaa acctaagaga aaagagcgtt
       61 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
      121 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
      181 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg
      241 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
      301 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
      361 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
      421 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
      481 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
      541 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
      601 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
      661 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
      721 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
      781 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
      841 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
      901 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
      961 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     1021 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     1081 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     1141 acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     1201 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     1261 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     1321 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     1381 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     1441 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     1501 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     1561 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     1621 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     1681 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     1741 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     1801 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     1861 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     1921 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     1981 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     2041 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     2101 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     2161 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     2221 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     2281 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     2341 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     2401 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     2461 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     2521 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     2581 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     2641 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     2701 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     2761 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     2821 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     2881 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     2941 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     3001 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     3061 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     3121 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     3181 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     3241 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     3301 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     3361 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     3421 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     3481 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg actctttcct
     3541 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     3601 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     3661 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     3721 ctggcctgtg cataactgtc tggccagcgc acagccgaag tgctgcaaaa agcgcctacc
     3781 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     3841 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     3901 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     3961 cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga
     4021 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     4081 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     4141 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     4201 agaaaatacc gcatcaggcg ctcatccgct tcctcgctca ctgactcgct gcgctcggtc
     4261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4801 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5041 aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact catcgagcat
     5101 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     5161 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     5221 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     5281 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     5341 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     5401 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac
     5461 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     5521 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     5581 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     5641 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     5701 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     5761 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     5821 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     5881 ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag tatttaaaga
     5941 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     6001 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     6061 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     6121 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     6181 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     6241 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     6301 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acataacgaa
     6361 ttgctcttca gcaaggagGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT
     6421 GTCAAAGATA CAGTCTCAGA AGATCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT
     6481 TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA
     6541 GAAAAGGAAG GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA
     6601 GATCTCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA
     6661 AAAGAAGAGG TTCCAACCAC GTCTACAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC
     6721 GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT
     6781 TCATTTCATT TGGAGAGGAC ACGCTCGAGT ATAAGAGCTC ATTTTTACAA CAATTACCAA
     6841 CAACAACAAA CAACAAACAA CATTACAATT ACATTTACAA TTATCGATAC AATGGGGAGA
     6901 GGGAAGATAG TTATACGAAG GATCGATAAC TCTACAAGTA GACAAGTGAC TTTCTCCAAG
     6961 AGAAGGAGTG GTTTGCTTAA GAAAGCTAAA GAGTTATCGA TCCTTTGTGA TGCAGAAGTT
     7021 GGTGTTATCA TATTCTCTAG CACCGGAAAG CTCTACGACT ACGCAAGCAA TTCAAGTATG
     7081 AAAACAATCA TTGAGCGGTA CAACAGAGTA AAAGAGGAGC AGCATCAACT TCTGAATCAT
     7141 GCCTCAGAGA TAAAGTTTTG GCAAAGAGAG GTTGCAAGTT TGCAGCAGCA GCTCCAATAT
     7201 CTACAAGAAT GCCACAGGAA ACTAGTGGGA GAGGAACTTT CTGGAATGAA TGCTAACGAC
     7261 CTACAAAACC TTGAAGACCA GCTAGTAACA AGTCTAAAAG GTGTTCGTCT CAAAAAGGAT
     7321 CAACTTATGA CAAATGAAAT CAGAGAACTT AATCGTAAGG GACAAATCAT CCAAAAAGAG
     7381 AATCACGAGC TACAAAATAT TGTAGATATA ATGCGTAAGG AAAATATTAA ATTGCAAAAG
     7441 AAGGTTCATG GAAGAACAAA TGCGATTGAA GGCAATTCAA GTGTAGATCC AATAAGCAAT
     7501 GGAACCACAA CATATGCACC ACCGCAACTT CAACTCATAC AACTACAACC AGCTCCTAGA
     7561 GAAAAATCAA TCAGACTAGG AATATGTCTG AATCTTtgag cttCTCTAGC TAGAGTCGAT
     7621 CGACAAGCTC GAGTTTCTCC ATAATAATGT GTGAGTAGTT CCCAGATAAG GGAATTAGGG
     7681 TTCCTATAGG GTTTCGCTCA TGTGTTGAGC ATATAAGAAA CCCTTAGTAT GTATTTGTAT
     7741 TTGTAAAATA CTTCTATCAA TAAAATTTCT AATTCCTAAA ACCAAAATCC AGTACTAAAA
     7801 TCCAGATCGC T
//