LOCUS       pAcBac_Ma_sfGFP        9795 bp DNA     circular SYN 28-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   OSU CoSINe
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..515
                     /label=Ma pylT cassette 1
                     /note="This feature has 2 segments:
                        1:   1 .. 357 / #a6acb3
                        2: 358 .. 515 / #a6acb3"
     misc_feature    1..361
                     /label=Ma pylT gB2
                     /note="This feature has 2 segments:
                        1:   1 .. 357 / #ccffff
                        2: 358 .. 361 / #ccffff"
     promoter        17..257
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    266..334
                     /label=Ma Pyl tRNA(6)
                     /note="color: #ffcc99"
     misc_feature    342..515
                     /label=Ma pylT gB1
                     /note="This feature has 2 segments:
                        1: 342 .. 357 / #ff99cc
                        2: 358 .. 515 / #ff99cc"
     promoter        342..436
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
                     /note="This forward directional feature has 2 segments:
                        1: 342 .. 357 / #ffffff
                        2: 358 .. 436 / #ffffff"
     protein_bind    390..408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    418..436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    441..509
                     /label=Ma Pyl tRNA(6)
                     /note="color: #ffcc99"
     primer_bind     511..532
                     /label=pAcBac-OriBits-for-PaqCl-GG
                     /note="color: black; sequence: 
                     TCTACCCACCTGCctctttttcagctggggctctaggg"
     misc_feature    512..515
                     /label=ov1
                     /note="color: #a6acb3"
     polyA_site      568..587
                     /label=HSV tk PolyA
                     /note="color: #a6acb3"
     primer_bind     588..607
                     /label=strip down R
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(638..658)
                     /label=sqF
                     /note="color: #a020f0; direction: LEFT"
     promoter        927..955
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1144..1677
                     /label=Gentamicin Resistance
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1744..1968
                     /label=Tn7R
                     /note="color: #a6acb3"
     rep_origin      2253..2935
                     /label=ColE1 origin
                     /note="color: #ffffff"
     CDS             complement(3030..3890)
                     /label=AmpR
                     /note="color: #ffffff; direction: LEFT"
     promoter        3891..3995
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     rep_origin      4022..4477
                     /label=M13 origin
                     /note="color: #ffffff"
     rep_origin      4032..4472
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #ffffff"
     mobile_element  4661..4826
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #a6acb3"
     misc_feature    4661..4826
                     /label=Tn7L
                     /note="color: #a6acb3"
     primer_bind     4774..4808
                     /label=sqR
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4814..4839)
                     /label=pAcBac-OriBits-rev-PaqCl-GG
                     /note="color: black; sequence: 
                     GGTAGACACCTGCagaggctaggagatccgaaccagataagtg"
     misc_feature    4840..5343
                     /label=Ma pylT cassette 2
                     /note="This feature has 2 segments:
                        1: 4840 .. 5003 / #a6acb3
                        2: 5004 .. 5343 / #a6acb3"
     misc_feature    4840..5013
                     /label=Ma pylT gB4
                     /note="This feature has 2 segments:
                        1: 4840 .. 5003 / #cc99ff
                        2: 5004 .. 5013 / #cc99ff"
     misc_feature    4846..4914
                     /label=Ma Pyl tRNA(6)
                     /note="color: #ffff99"
     promoter        complement(4919..5013)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
                     /note="This reverse directional feature has 2 segments:
                        1: 4919 .. 5003 / #ffffff
                        2: 5004 .. 5013 / #ffffff"
     protein_bind    4919..4937
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4947..4965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    4994..5343
                     /label=Ma pylT gB3
                     /note="This feature has 2 segments:
                        1: 4994 .. 5003 / #99ccff
                        2: 5004 .. 5343 / #99ccff"
     misc_feature    5021..5089
                     /label=Ma Pyl tRNA(6)
                     /note="color: #ffff99"
     promoter        complement(5098..5338)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     5335..5358
                     /label=pAcBac-RS-for-PaqCl-GG
                     /note="color: black; sequence: 
                     TCTACCCACCTGCctcttcctgcccgacctaggctc"
     misc_feature    5340..5343
                     /label=ov3
                     /note="color: #a6acb3"
     misc_feature    complement(5344..5349)
                     /label=U6
                     /label=U6(1)
                     /note="U6"
                     /note="color: #ff9900; direction: LEFT"
     polyA_signal    5371..5505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    5375..5566
                     /label=SV40 late polyA
                     /note="color: #ffffff; direction: RIGHT"
     polyA_site      5375..5505
                     /label=SV40 Early PolyA(1)
                     /note="color: #a6acb3"
     polyA_signal    5440..5474
                     /label=SV40 Early PolyA
                     /note="color: #a6acb3"
     CDS             complement(5651..7186)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /note="color: #ffffff"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     misc_feature    7187..7207
                     /label=L21 sequence
                     /note="L21 sequence"
                     /note="color: #a6acb3"
     promoter        complement(7245..7336)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7428..8015
                     /label=CMV promoter
                     /label=CMV
                     /note="CMV"
                     /note="color: #ffff00; direction: RIGHT"
     enhancer        7431..7810
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        7811..8014
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     7965..7985
                     /label=CMV-F
                     /note="color: #a020f0; direction: RIGHT"
     promoter        8059..8077
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     8065..8115
                     /label=pAcBac_sfGFP_for
                     /note="color: black; sequence: 
                     gactcactatagggagacccaagctggctagcaccatggtttctaaaggtg"
     regulatory      8096..8103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             8100..8822
                     /codon_start=1
                     /label=sfGFP
                     /label=GFP
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYKGS"
     CDS             8823..8864
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /note="color: #993366"
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(8860..8915)
                     /label=pAcBac_sfGFP_rev
                     /note="color: black; sequence: 
                     ggttgattgtcgacttaacgcgttgaattcttagtggtgatggtggtgatgagtag"
     CDS             8865..8882
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #993366"
                     /translation="HHHHHH"
     primer_bind     complement(8882..8916)
                     /label=C-term-EcoRI-5458
                     /note="color: black; sequence: 
                     aggttgattgtcgacttaacgcgttgaattcttag"
     misc_feature    8908..9496
                     /label=WPRE
                     /note="WPRE"
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(9545..9562)
                     /label=BghR
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    9551..9775
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #ffff00"
     primer_bind     complement(9776..9795)
                     /label=pAcBac-RS-rev-PaqCI-GG
                     /note="color: black; sequence: 
                     GGTAGACACCTGCagagggttctttccgcctcagaag"
ORIGIN
        1 agatctcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
       61 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
      121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      241 tatcttgtgg aaaggacgaa acaccggggg acggtccggc gaccagcggg tctctaaaac
      301 ctagcatagc ggggttcgac accccggtct ctcgtttttt aatatttgca tgtcgctatg
      361 tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc
      421 ctatcagtga tagagacacc gggggacggt ccggcgacca gcgggtctct aaaacctagc
      481 atagcggggt tcgacacccc ggtctctcgt tttttcagct ggggctctag ggggtatccc
      541 cacgcgccct gtagcggcgc attaagcgca agccctgcca tagccactac gggtacgtag
      601 gccaaccact agaactatag ctagagtcct gggcgaacaa acgatgctcg ccttccagaa
      661 aaccgaggat gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc
      721 gaggtctacc gatctcctga agccagggca gatccgtgca cagcaccttg ccgtagaaga
      781 acagcaaggc cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca
      841 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt
      901 ggaaacggat gaaggcacga acccagttga cataagcctg ttcggttcgt aaactgtaat
      961 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1021 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag tctatgcctc
     1081 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     1141 acgatgttac gcagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     1201 ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc
     1261 atgcgggctg ctcttgatct tttcggtcgt gagttcggag acgtagccac ctactcccaa
     1321 catcagccgg actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt
     1381 gctgccttcg accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaggttt
     1441 gagcagccgc gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg
     1501 cagggcattg ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct
     1561 tatgtgatct acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag
     1621 ttgggcatac gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaacaa
     1681 ttcgttcaag ccgagatcgg cttcccggcc gcggagttgt tcggtaaatt gtcacaacgc
     1741 cgcgaatata gtctttacca tgcccttggc cacgcccctc tttaatacga cgggcaattt
     1801 gcacttcaga aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac
     1861 agcataactg gactgatttc agtttacaac tattctgtct agtttaagac tttattgtca
     1921 tagtttagat ctattttgtt cagtttaaga ctttattgtc cgcccacacc cgcttacgca
     1981 gggcatccat ttattactca accgtaaccg attttgccag gttacgcggc tggtctgcgg
     2041 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     2101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2161 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2221 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2281 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2341 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2401 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2461 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2521 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2581 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2641 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2701 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2761 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2821 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2881 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2941 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3001 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3061 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3901 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3961 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4021 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4081 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4141 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4201 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4261 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4321 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4381 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4441 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcaa
     4501 ataagcgttg atattcagtc aattacaaac attaataacg aagagatgac agaaaaattt
     4561 tcattctgtg acagagaaaa agtagccgaa gatgacggtt tgtcacatgg agttggcagg
     4621 atgtttgatt aaaaacataa caggaagaaa aatgccccgc tgtgggcgga caaaatagtt
     4681 gggaactggg aggggtggaa atggagtttt taaggattat ttagggaaga gtgacaaaat
     4741 agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa ttaaaaatga caaaatagtt
     4801 tggaactaga tttcacttat ctggttcgga tctcctagca aaaaacgaga gaccggggtg
     4861 tcgaaccccg ctatgctagg ttttagagac ccgctggtcg ccggaccgtc ccccggtgtc
     4921 tctatcactg atagggaact tataagtctc tatcactgat agggatttca cgtttatggt
     4981 gatttcccag aacacatagc gacatgcaaa tattaaaaaa cgagagaccg gggtgtcgaa
     5041 ccccgctatg ctaggtttta gagacccgct ggtcgccgga ccgtcccccg gtgtttcgtc
     5101 ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat
     5161 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc
     5221 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct
     5281 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt
     5341 cctgcccgac ctaggctcaa gcagtgatca gatccagaca tgataagata cattgatgag
     5401 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5461 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5521 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     5581 ctctacaaat gtggtatggc tgattatgat cctctagtac ttctcgacaa gcttggccgg
     5641 cctggcctta tcactttcca agtcggttca tctctatgtc tgtataaatc tgtcttttct
     5701 tggtgtgctt taatttaatg caaagatgga taccaactcg gagaaccaag aatagtccaa
     5761 tgattaaccc tatgataaag aaaaaagagg caatagagct tttccaacta ctgaaccaac
     5821 cttctacaag ctcgattgga tttttggata gcccagtatc accaaaaaat aaactctcat
     5881 catcaggaag ttgcgaagca gcgtcttgaa tgtgaggatg ttcgaacacc tgagcctttg
     5941 agctaagatg aagatcggag tccaacatac catgtccaat catgtataaa ggaaacttat
     6001 atcctgaact ggtcctcaga actccattgg gtccaatttc cacgtcttca tatggtgccc
     6061 agtcatccca cagttccctt tctgtggtag ttccactgat cattccgacc attcttgaga
     6121 ggattggagc agcaatatcg actctgatgt atctggtctc aaagtatttt agggtaccat
     6181 tgattatggt gaaagcagga ccggttcctg ggtttttagg agcaagatag ctgagatcca
     6241 ctggagagat tggaagaccc gctctgattt tgctccaggt ttcttggcag agggaataat
     6301 ccaagatcct ctcaacgtcc tgaattagac ttacatccac tgaggtctga gatggagcag
     6361 agatacttga cccttctggg cattcaggga atctggctgc agcaaagaga tccttatcag
     6421 ccatctcgaa ccagacacct gatgggagtc tgactcccca atgcttgcag tattgcattt
     6481 tgcaggcctt gcctccagtt tcataagcaa agtagttact tctgaaccct gtgccctcct
     6541 ttcccaggga tgatagctct ccgtcctctg agaagaaggt gatgtccatg gaaatgaggt
     6601 tagaatcaca tagccctttg accttatagt cagaatgcca ggttgtagag ttatggacag
     6661 tggggcatat gtaattgctg cattttccgt tgatgaactg tgaatcaacc cattctcctg
     6721 tgtattcatc aaccagcaca tggtgaggag tcacctggac aatcactgct tcggcatccg
     6781 tcacagttgc atatccacaa ctttgaggag ggaagcctgg attcagccaa gttccttgtt
     6841 tcgtttgttc aatgctttcc ttgcattgtt ctacagatgg agtgaaggat cggatggaat
     6901 gtgttatata cttcggtcca taccagcgga aatcacaagt agtgacccat ttggaagcat
     6961 gacacatcca accgtctgct tgaatagcct tgtgactctt gggcattttg acttgtaagg
     7021 ctgtgcctat taagtcatta tgccaattta aatctgagct tgacgggcaa taatggtaat
     7081 tagaaggaac atttttccag tttccttttt ggttgtgtgg aaaaactatg gtgaacttgc
     7141 aattcacccc aatgaataaa aaggctaagt acaaaaggca cttcatggtg gcggtttttt
     7201 aggagttcgc gcccgatggt gggacggtat gaataatccg gaatatttat aggttttttt
     7261 attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt
     7321 taattatctc catgatctat taatattccg gagtatacgg accgcggccg caaatacctg
     7381 caggatccgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     7441 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     7501 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     7561 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     7621 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     7681 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     7741 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     7801 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     7861 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     7921 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     7981 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
     8041 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcacca
     8101 tggtttctaa aggtgaagaa ctttttactg gcgttgtgcc gattctggtg gaactggatg
     8161 gtgatgtgaa tggccataaa tttagcgttc gtggcgaagg cgaaggtgat gcgaccaacg
     8221 gtaaactgac cctgaaattt atttgcacca ccggtaaact gccggttccg tggccgaccc
     8281 tggtgaccac cctgacctat ggcgttcagt gctttagccg ctatccggat catatgaaac
     8341 gccatgattt ctttaaaagc gcgatgccgg aaggctatgt gcaggaacgt accattagct
     8401 tcaaagatga tggcacctat aaaacccgtg cggaagttaa atttgaaggc gataccctgg
     8461 tgaaccgcat tgaactgaaa ggtattgatt ttaaagaaga tggcaacatt ctgggtcata
     8521 aactggaata taatttcaac agccataatg tgtatattac cgccgataaa cagaaaaatg
     8581 gcatcaaagc gaactttaaa atccgtcaca acgtggaaga tggtagcgtg cagctggcgg
     8641 atcattatca gcagaatacc ccgattggtg atggcccggt gctgctgccg gataatcatt
     8701 atctgagcac ccagagcgtt ctgagcaaag atccgaatga aaaacgtgat catatggtgc
     8761 tgctggaatt tgttaccgcc gcgggcatta cccacggtat ggatgaactt tataaaggtt
     8821 ctggaaagcc gattccaaat cccctgttgg gactggattc tactcatcac caccatcacc
     8881 actaagaatt caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8941 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     9001 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9061 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9121 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9181 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9241 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9301 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9361 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9421 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9481 tgggccgcct ccccgcgtcg actttaactc gagtctagag ggcccgttta aacccgctga
     9541 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     9601 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9661 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9721 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9781 gaggcggaaa gaacc
//
