LOCUS       pAcBac1-Mb-sfGFP        9795 bp DNA     circular SYN 02-MAY-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pAcBac1-sfGFP-150TAG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          1..3517
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /label=source:synthetic DNA construct(1)
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          4313..9795
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     mobile_element  83..248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #a6acb3"
     misc_feature    83..248
                     /label=Tn7L
                     /note="color: #a6acb3"
     primer_bind     196..230
                     /label=sqR
                     /note="color: #a020f0; direction: RIGHT"
     tRNA            complement(268..336)
                     /label=DhPyl3M
                     /note="DhPyl3M"
                     /note="color: #00ffff; direction: LEFT"
     promoter        341..435
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
                     /note="color: #ffffff"
     promoter        complement(341..435)
                     /label=H1-2O2
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
                     /note="color: #c0c0c0; direction: LEFT"
     protein_bind    341..359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     tRNA            complement(443..511)
                     /label=MmPylT-c
                     /note="MmPylT-c"
                     /note="color: #0000ff; direction: LEFT"
     promoter        complement(512..771)
                     /label=U6
                     /label=U6(1)
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #c0c0c0; direction: LEFT"
     promoter        520..760
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff"
     polyA_signal    793..927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    797..988
                     /label=SV40 late polyA
                     /note="color: #ffffff; direction: RIGHT"
     polyA_site      797..927
                     /label=SV40 Early PolyA(1)
                     /note="color: #a6acb3"
     polyA_signal    862..896
                     /label=SV40 Early PolyA
                     /note="color: #a6acb3"
     CDS             complement(1073..2608)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /note="color: #ffffff"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     misc_feature    2609..2629
                     /label=L21 sequence
                     /note="L21 sequence"
                     /note="color: #a6acb3"
     promoter        2667..2758
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff"
     misc_feature    2850..3437
                     /label=CMV promoter
                     /label=CMV
                     /note="CMV"
                     /note="color: #808000; direction: RIGHT"
     enhancer        2853..3232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        3233..3436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3387..3407
                     /label=CMV-F
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3481..3499
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3487..3537
                     /label=sfGFPFwd
                     /note="color: black; sequence: 
                     gactcactatagggagacccaagctggctagcaccatggtttctaaaggtg; added:
                     2022-02-25"
     primer_bind     3501..3540
                     /label=S203Y For
                     /note="color: black; sequence: 
                     agacccaagctggctagcaccatggtttctaaaggtgaag; added: 
                     2022-02-14"
     regulatory      3518..3525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             3522..4244
                     /codon_start=1
                     /label=sfGFP
                     /label=GFP
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSH*VYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLYTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYKGS"
     misc_difference 3969..3971
                     /label=N150TAG
                     /note="AAT>TAG
                     Asn>Amber"
                     /note="color: #ff0000"
     primer_bind     4117..4141
                     /label=sfGFP-S203Y Fwd
                     /note="color: black; sequence: ATCATTATCTGTATACCCAGAGCGT; 
                     added: 2021-09-09"
     primer_bind     complement(4117..4141)
                     /label=sfGFP-S203Y Rev
                     /note="color: black; sequence: ACGCTCTGGGTATACAGATAATGAT; 
                     added: 2021-09-09"
     primer_bind     complement(4222..4329)
                     /label=sfGFP_V5_del_FLAGadd_rev
                     /note="color: black; sequence: 
                     gtcgacttaacgcgttgaattcttagtggtgatggtggtgatgcttatcgtcgtcatcc
                     ttgtaatcagaacctttataaagttcatcca; added: 2022-05-02"
     primer_bind     complement(4224..4263)
                     /label=S203Y rev
                     /note="color: black; sequence: 
                     gatttggaatcggctttccagaacctttataaagttcatc; added: 
                     2022-02-14"
     CDS             4245..4286
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /note="color: #993366"
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4282..4337)
                     /label=sfGFPREV
                     /note="color: black; sequence: 
                     ggttgattgtcgacttaacgcgttgaattcttagtggtgatggtggtgatgagtag; 
                     added: 2022-02-25"
     CDS             4287..4304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #993366"
                     /translation="HHHHHH"
     primer_bind     complement(4304..4338)
                     /label=C-term-EcoRI-5458
                     /note="color: black; sequence: 
                     aggttgattgtcgacttaacgcgttgaattcttag; added: 2019-10-25"
     misc_feature    4330..4918
                     /label=WPRE
                     /note="WPRE"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     complement(4967..4984)
                     /label=BghR
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    4973..5197
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #808000"
     promoter        5223..5482
                     /label=U6
                     /label=U6(1)
                     /note="U6"
                     /note="color: #c0c0c0; direction: RIGHT"
     tRNA            5483..5551
                     /label=MmPylT-c
                     /note="MmPylT-c"
                     /note="color: #0000ff; direction: RIGHT"
     promoter        5558..5657
                     /label=H1
                     /label=H1(1)
                     /note="H1"
                     /note="color: #c0c0c0; direction: RIGHT"
     protein_bind    5607..5625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5635..5653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     tRNA            5658..5726
                     /label=DhPyl3M
                     /note="DhPyl3M"
                     /note="color: #00ffff; direction: RIGHT"
     polyA_site      5785..5804
                     /label=HSV tk PolyA
                     /note="color: #a6acb3"
     primer_bind     5805..5824
                     /label=strip down R
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(5855..5875)
                     /label=sqF
                     /note="color: #a020f0; direction: LEFT"
     promoter        6144..6172
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    6361..6894
                     /label=Gentamicin Resistance
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    6961..7185
                     /label=Tn7R
                     /note="color: #a6acb3"
     rep_origin      7470..8152
                     /label=ColE1 origin
                     /note="color: #ffffff"
     CDS             complement(8247..9107)
                     /label=AmpR
                     /note="color: #ffffff; direction: LEFT"
     promoter        9108..9212
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     rep_origin      9239..9694
                     /label=M13 origin
                     /note="color: #ffffff"
     rep_origin      9249..9689
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #ffffff"
ORIGIN
        1 aaagtagccg aagatgacgg tttgtcacat ggagttggca ggatgtttga ttaaaaacat
       61 aacaggaaga aaaatgcccc gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg
      121 aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta
      181 gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt
      241 atctggttcg gatctcctag caaaaaacgg ggggtgcggg agtttcaccc gcctgcacgg
      301 atttagagtc cgtgtgatct attcgatcca ccccccggtg tctctatcac tgatagggaa
      361 cttataagtc tctatcactg atagggattt cacgtttatg gtgatttccc agaacacata
      421 gcgacatgca aatattaaaa aacggaaacc ccgggaatct aacccggctg aacggattta
      481 gagtccgttc gatctacatg atcaggtttc cggtgtttcg tcctttccac aagatatata
      541 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      601 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      661 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      721 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg acctaggctc
      781 aagcagtgat cagatccaga catgataaga tacattgatg agtttggaca aaccacaact
      841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      961 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1021 gctgattatg atcctctagt acttctcgac aagcttggcc ggcctggcct tatcactttc
     1081 caagtcggtt catctctatg tctgtataaa tctgtctttt cttggtgtgc tttaatttaa
     1141 tgcaaagatg gataccaact cggagaacca agaatagtcc aatgattaac cctatgataa
     1201 agaaaaaaga ggcaatagag cttttccaac tactgaacca accttctaca agctcgattg
     1261 gatttttgga tagcccagta tcaccaaaaa ataaactctc atcatcagga agttgcgaag
     1321 cagcgtcttg aatgtgagga tgttcgaaca cctgagcctt tgagctaaga tgaagatcgg
     1381 agtccaacat accatgtcca atcatgtata aaggaaactt atatcctgaa ctggtcctca
     1441 gaactccatt gggtccaatt tccacgtctt catatggtgc ccagtcatcc cacagttccc
     1501 tttctgtggt agttccactg atcattccga ccattcttga gaggattgga gcagcaatat
     1561 cgactctgat gtatctggtc tcaaagtatt ttagggtacc attgattatg gtgaaagcag
     1621 gaccggttcc tgggttttta ggagcaagat agctgagatc cactggagag attggaagac
     1681 ccgctctgat tttgctccag gtttcttggc agagggaata atccaagatc ctctcaacgt
     1741 cctgaattag acttacatcc actgaggtct gagatggagc agagatactt gacccttctg
     1801 ggcattcagg gaatctggct gcagcaaaga gatccttatc agccatctcg aaccagacac
     1861 ctgatgggag tctgactccc caatgcttgc agtattgcat tttgcaggcc ttgcctccag
     1921 tttcataagc aaagtagtta cttctgaacc ctgtgccctc ctttcccagg gatgatagct
     1981 ctccgtcctc tgagaagaag gtgatgtcca tggaaatgag gttagaatca catagccctt
     2041 tgaccttata gtcagaatgc caggttgtag agttatggac agtggggcat atgtaattgc
     2101 tgcattttcc gttgatgaac tgtgaatcaa cccattctcc tgtgtattca tcaaccagca
     2161 catggtgagg agtcacctgg acaatcactg cttcggcatc cgtcacagtt gcatatccac
     2221 aactttgagg agggaagcct ggattcagcc aagttccttg tttcgtttgt tcaatgcttt
     2281 ccttgcattg ttctacagat ggagtgaagg atcggatgga atgtgttata tacttcggtc
     2341 cataccagcg gaaatcacaa gtagtgaccc atttggaagc atgacacatc caaccgtctg
     2401 cttgaatagc cttgtgactc ttgggcattt tgacttgtaa ggctgtgcct attaagtcat
     2461 tatgccaatt taaatctgag cttgacgggc aataatggta attagaagga acatttttcc
     2521 agtttccttt ttggttgtgt ggaaaaacta tggtgaactt gcaattcacc ccaatgaata
     2581 aaaaggctaa gtacaaaagg cacttcatgg tggcggtttt ttaggagttc gcgcccgatg
     2641 gtgggacggt atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga
     2701 aaacagtaaa atacttattt atttgcgaga tggttatcat tttaattatc tccatgatct
     2761 attaatattc cggagtatac ggaccgcggc cgcaaatacc tgcaggatcc gttttgcgct
     2821 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     2881 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2941 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3001 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta
     3061 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3121 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3181 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     3241 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3301 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3361 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3421 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     3481 taatacgact cactataggg agacccaagc tggctagcac catggtttct aaaggtgaag
     3541 aactttttac tggcgttgtg ccgattctgg tggaactgga tggtgatgtg aatggccata
     3601 aatttagcgt tcgtggcgaa ggcgaaggtg atgcgaccaa cggtaaactg accctgaaat
     3661 ttatttgcac caccggtaaa ctgccggttc cgtggccgac cctggtgacc accctgacct
     3721 atggcgttca gtgctttagc cgctatccgg atcatatgaa acgccatgat ttctttaaaa
     3781 gcgcgatgcc ggaaggctat gtgcaggaac gtaccattag cttcaaagat gatggcacct
     3841 ataaaacccg tgcggaagtt aaatttgaag gcgataccct ggtgaaccgc attgaactga
     3901 aaggtattga ttttaaagaa gatggcaaca ttctgggtca taaactggaa tataatttca
     3961 acagccatta ggtgtatatt accgccgata aacagaaaaa tggcatcaaa gcgaacttta
     4021 aaatccgtca caacgtggaa gatggtagcg tgcagctggc ggatcattat cagcagaata
     4081 ccccgattgg tgatggcccg gtgctgctgc cggataatca ttatctgtat acccagagcg
     4141 ttctgagcaa agatccgaat gaaaaacgtg atcatatggt gctgctggaa tttgttaccg
     4201 ccgcgggcat tacccacggt atggatgaac tttataaagg ttctggaaag ccgattccaa
     4261 atcccctgtt gggactggat tctactcatc accaccatca ccactaagaa ttcaacgcgt
     4321 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4381 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4441 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4501 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4561 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4621 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4681 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4741 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4801 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4861 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     4921 cgactttaac tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4981 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5041 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5101 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5161 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccaga
     5221 tctcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     5281 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     5341 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     5401 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     5461 cttgtggaaa ggacgaaaca ccggaaacct gatcatgtag atcgaacgga ctctaaatcc
     5521 gttcagccgg gttagattcc cggggtttcc gttttttaat atttgcatgt cgctatgtgt
     5581 tctgggaaat caccataaac gtgaaatccc tatcagtgat agagacttat aagttcccta
     5641 tcagtgatag agacaccggg gggtggatcg aatagatcac acggactcta aatccgtgca
     5701 ggcgggtgaa actcccgcac cccccgtttt ttcagctggg gctctagggg gtatccccac
     5761 gcgccctgta gcggcgcatt aagcgcaagc cctgccatag ccactacggg tacgtaggcc
     5821 aaccactaga actatagcta gagtcctggg cgaacaaacg atgctcgcct tccagaaaac
     5881 cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag
     5941 gtctaccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca
     6001 gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
     6061 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
     6121 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca
     6181 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     6241 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg
     6301 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     6361 atgttacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaggt
     6421 ggctcaagta tgggcatcat tcgcacatgt aggctcggcc ctgaccaagt caaatccatg
     6481 cgggctgctc ttgatctttt cggtcgtgag ttcggagacg tagccaccta ctcccaacat
     6541 cagccggact ccgattacct cgggaacttg ctccgtagta agacattcat cgcgcttgct
     6601 gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc caggtttgag
     6661 cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggcgagca ccggaggcag
     6721 ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct tggtgcttat
     6781 gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta tacaaagttg
     6841 ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac ctaacaattc
     6901 gttcaagccg agatcggctt cccggccgcg gagttgttcg gtaaattgtc acaacgccgc
     6961 gaatatagtc tttaccatgc ccttggccac gcccctcttt aatacgacgg gcaatttgca
     7021 cttcagaaaa tgaagagttt gctttagcca taacaaaagt ccagtatgct ttttcacagc
     7081 ataactggac tgatttcagt ttacaactat tctgtctagt ttaagacttt attgtcatag
     7141 tttagatcta ttttgttcag tttaagactt tattgtccgc ccacacccgc ttacgcaggg
     7201 catccattta ttactcaacc gtaaccgatt ttgccaggtt acgcggctgg tctgcggtgt
     7261 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7681 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7921 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     9241 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     9301 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9361 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9421 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9481 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9541 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9601 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9661 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcaaata
     9721 agcgttgata ttcagtcaat tacaaacatt aataacgaag agatgacaga aaaattttca
     9781 ttctgtgaca gagaa
//
