LOCUS       pAcBac1-NES-Flag       10350 bp DNA     circular SYN 18-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Hyo Sang Jang
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  83..248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #a6acb3"
     misc_feature    83..248
                     /label=Tn7L
                     /note="color: #a6acb3"
     primer_bind     196..230
                     /label=sqR
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    complement(268..336)
                     /label=DhPylT-3M
                     /note="DhPylT-3M"
                     /note="color: #ff9900; direction: LEFT"
     misc_feature    complement(337..436)
                     /label=H1
                     /label=H1(1)
                     /note="H1"
                     /note="color: #ff9900; direction: LEFT"
     promoter        341..435
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
                     /note="color: #ffffff"
     protein_bind    341..359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    complement(443..511)
                     /label=MmPylT-c
                     /note="MmPylT-c"
                     /note="color: #ff9900; direction: LEFT"
     misc_feature    complement(512..771)
                     /label=U6
                     /label=U6(1)
                     /note="U6"
                     /note="color: #ff9900; direction: LEFT"
     promoter        520..760
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff"
     polyA_signal    793..927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    797..988
                     /label=SV40 late polyA
                     /note="color: #ffffff; direction: RIGHT"
     polyA_site      797..927
                     /label=SV40 Early PolyA(1)
                     /note="color: #a6acb3"
     polyA_signal    862..896
                     /label=SV40 Early PolyA
                     /note="color: #a6acb3"
     CDS             complement(1073..2608)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /note="color: #ffffff"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     misc_feature    2609..2629
                     /label=L21 sequence
                     /note="L21 sequence"
                     /note="color: #a6acb3"
     promoter        complement(2667..2758)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    2850..3437
                     /label=CMV promoter
                     /label=CMV
                     /note="CMV"
                     /note="color: #993366; direction: RIGHT"
     enhancer        2853..3232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        3233..3436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3387..3407
                     /label=CMV-F
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3481..3499
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3513..3556
                     /label=JP9
                     /note="color: black; sequence: 
                     taaacttgctagcgccaccatggcgtgtccggttcctttgcagttgcctcc; added:
                     2019-08-09"
     primer_bind     3513..3556
                     /label=JP1
                     /note="color: black; sequence: 
                     taaacttgctagcgccgccaccatggcgtgtccggttcctttgcagttgcctcc; 
                     added: 2019-08-09"
     regulatory      3519..3528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             3525..3548
                     /codon_start=1
                     /label=start
                     /note="color: #993366"
                     /translation="MACPVPLQ"
     CDS             3549..3575
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             3603..4859
                     /codon_start=1
                     /label=B5 F-Phe Mb RS
                     /note="color: #993366"
                     /translation="DKKPLDVLISATGLWMSRTGTLHKIKHHEVSRSKIYIEMACGDHL
                     VVNNSRSCRTARAFRHHKYRKTCKRCRVSDEDINNFLTRSTESKNSVKVRVVSAPKVKK
                     AMPKSVSRAPKPLENSVSAKASTNTSRSVPSPAKSTPNSSVPASAPAPSLTRSQLDRVE
                     ALLSPEDKISLNMAKPFRELEPELVTRRKNDFQRLYTNDREDYLGKLERDITKFFVDRG
                     FLEIKSPILIPAEYVERMGINNDTELSKQIFRVDKNLCLRPMLAPTLYNYLRKLDRILP
                     GPIKIFEVGPCYRKESDGKEHLEEFTMVGFAQMGSGCTRENLEALIKEFLDYLEIDFEI
                     VGDSCMVYGDTLDIMHGDLELSSAVVGPVSLDREWGIDKPWIGAGFGLERLLKVMHGFK
                     NIKRASRSESYYNGISTNL"
     primer_bind     4379..4424
                     /label=JP3
                     /note="color: black; sequence: 
                     gtgcctgcggcctaccctggcccccacctcactcaactacctgcgg; added: 
                     2019-08-09"
     primer_bind     complement(4379..4424)
                     /label=JP2
                     /note="color: black; sequence: 
                     ccgcaggtagttgagtgaggtgggggccagggtaggccgcaggcac; added: 
                     2019-08-09"
     primer_bind     4517..4555
                     /label=JP5
                     /note="color: black; sequence: 
                     gtttacaatggtgggttttacccagatgggcagcggctg; added: 2019-08-09"
     primer_bind     complement(4517..4554)
                     /label=JP4
                     /note="color: black; sequence: 
                     caagccgctgcccatctgggtaaaacccaccattgtaaac; added: 
                     2019-08-09"
     primer_bind     complement(4822..4868)
                     /label=JP10
                     /note="color: black; sequence: 
                     agtggagaattctcatcacaggttggtgctgatgccgttgtagtagctctcgc; 
                     added: 2019-08-08"
     primer_bind     complement(4828..4862)
                     /label=JP6
                     /note="color: black; sequence: 
                     cagcgggtttaaacgggccctctagtcatcacaggttggtgctgatgccgttgtagtag
                     c; added: 2019-08-08"
     misc_feature    4860..4862
                     /label=*
                     /note="color: #ff0000"
     misc_feature    4885..5473
                     /label=WPRE
                     /note="WPRE"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(5492..5516)
                     /label=JP6
                     /note="color: black; sequence: 
                     cagcgggtttaaacgggccctctagtcatcacaggttggtgctgatgccgttgtagtag
                     c; added: 2019-08-08"
     primer_bind     complement(5522..5539)
                     /label=BghR
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    5528..5752
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #993366"
     promoter        5789..6029
                     /label=U6
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    6038..6106
                     /label=MmPylTc
                     /note="MmPylTc"
                     /note="color: #00ff00; direction: RIGHT"
     promoter        6114..6208
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
                     /note="color: #00ff00; direction: RIGHT"
     protein_bind    6162..6180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    6190..6208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    6213..6281
                     /label=DhPyl3M
                     /note="DhPyl3M"
                     /note="color: #00ff00; direction: RIGHT"
     polyA_site      6340..6359
                     /label=HSV tk PolyA
                     /note="color: #a6acb3"
     primer_bind     6360..6379
                     /label=strip down R
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(6410..6430)
                     /label=sqF
                     /note="color: #a020f0; direction: LEFT"
     promoter        6699..6727
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    6916..7449
                     /label=Gentamicin Resistance
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    7516..7740
                     /label=Tn7R
                     /note="color: #a6acb3"
     rep_origin      8025..8707
                     /label=ColE1 origin
                     /note="color: #ffffff"
     CDS             complement(8802..9662)
                     /label=AmpR
                     /note="color: #ffffff; direction: LEFT"
     promoter        9663..9767
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     rep_origin      9794..10249
                     /label=M13 origin
                     /note="color: #ffffff"
     rep_origin      9804..10244
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #ffffff"
ORIGIN
        1 aaagtagccg aagatgacgg tttgtcacat ggagttggca ggatgtttga ttaaaaacat
       61 aacaggaaga aaaatgcccc gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg
      121 aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta
      181 gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt
      241 atctggttcg gatctcctag caaaaaacgg ggggtgcggg agtttcaccc gcctgcacgg
      301 atttagagtc cgtgtgatct attcgatcca ccccccggtg tctctatcac tgatagggaa
      361 cttataagtc tctatcactg atagggattt cacgtttatg gtgatttccc agaacacata
      421 gcgacatgca aatattaaaa aacggaaacc ccgggaatct aacccggctg aacggattta
      481 gagtccgttc gatctacatg atcaggtttc cggtgtttcg tcctttccac aagatatata
      541 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      601 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      661 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      721 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg acctaggctc
      781 aagcagtgat cagatccaga catgataaga tacattgatg agtttggaca aaccacaact
      841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      961 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1021 gctgattatg atcctctagt acttctcgac aagcttggcc ggcctggcct tatcactttc
     1081 caagtcggtt catctctatg tctgtataaa tctgtctttt cttggtgtgc tttaatttaa
     1141 tgcaaagatg gataccaact cggagaacca agaatagtcc aatgattaac cctatgataa
     1201 agaaaaaaga ggcaatagag cttttccaac tactgaacca accttctaca agctcgattg
     1261 gatttttgga tagcccagta tcaccaaaaa ataaactctc atcatcagga agttgcgaag
     1321 cagcgtcttg aatgtgagga tgttcgaaca cctgagcctt tgagctaaga tgaagatcgg
     1381 agtccaacat accatgtcca atcatgtata aaggaaactt atatcctgaa ctggtcctca
     1441 gaactccatt gggtccaatt tccacgtctt catatggtgc ccagtcatcc cacagttccc
     1501 tttctgtggt agttccactg atcattccga ccattcttga gaggattgga gcagcaatat
     1561 cgactctgat gtatctggtc tcaaagtatt ttagggtacc attgattatg gtgaaagcag
     1621 gaccggttcc tgggttttta ggagcaagat agctgagatc cactggagag attggaagac
     1681 ccgctctgat tttgctccag gtttcttggc agagggaata atccaagatc ctctcaacgt
     1741 cctgaattag acttacatcc actgaggtct gagatggagc agagatactt gacccttctg
     1801 ggcattcagg gaatctggct gcagcaaaga gatccttatc agccatctcg aaccagacac
     1861 ctgatgggag tctgactccc caatgcttgc agtattgcat tttgcaggcc ttgcctccag
     1921 tttcataagc aaagtagtta cttctgaacc ctgtgccctc ctttcccagg gatgatagct
     1981 ctccgtcctc tgagaagaag gtgatgtcca tggaaatgag gttagaatca catagccctt
     2041 tgaccttata gtcagaatgc caggttgtag agttatggac agtggggcat atgtaattgc
     2101 tgcattttcc gttgatgaac tgtgaatcaa cccattctcc tgtgtattca tcaaccagca
     2161 catggtgagg agtcacctgg acaatcactg cttcggcatc cgtcacagtt gcatatccac
     2221 aactttgagg agggaagcct ggattcagcc aagttccttg tttcgtttgt tcaatgcttt
     2281 ccttgcattg ttctacagat ggagtgaagg atcggatgga atgtgttata tacttcggtc
     2341 cataccagcg gaaatcacaa gtagtgaccc atttggaagc atgacacatc caaccgtctg
     2401 cttgaatagc cttgtgactc ttgggcattt tgacttgtaa ggctgtgcct attaagtcat
     2461 tatgccaatt taaatctgag cttgacgggc aataatggta attagaagga acatttttcc
     2521 agtttccttt ttggttgtgt ggaaaaacta tggtgaactt gcaattcacc ccaatgaata
     2581 aaaaggctaa gtacaaaagg cacttcatgg tggcggtttt ttaggagttc gcgcccgatg
     2641 gtgggacggt atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga
     2701 aaacagtaaa atacttattt atttgcgaga tggttatcat tttaattatc tccatgatct
     2761 attaatattc cggagtatac ggaccgcggc cgcaaatacc tgcaggatcc gttttgcgct
     2821 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     2881 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2941 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3001 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta
     3061 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3121 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3181 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     3241 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3301 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3361 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3421 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     3481 taatacgact cactataggg agacccaagc tggctagcgc caccatggcg tgtccggttc
     3541 ctttgcagtt gcctccactg gagcgcctca cactcgacga ctacaaggac gacgacgaca
     3601 aggacaagaa acccctggac gtgctgatca gcgccaccgg cctgtggatg agccggaccg
     3661 gcaccctgca caagatcaag caccacgagg tgtcaagaag caaaatctac atcgagatgg
     3721 cctgcggcga ccacctggtg gtgaacaaca gcagaagctg ccggaccgcc agagccttcc
     3781 ggcaccacaa gtacagaaag acctgcaagc ggtgccgggt gtccgacgag gacatcaaca
     3841 actttctgac cagaagcacc gagagcaaga acagcgtgaa agtgcgggtg gtgtccgccc
     3901 ccaaagtgaa gaaagccatg cccaagagcg tgtccagagc ccccaagccc ctggaaaaca
     3961 gcgtgtccgc caaggccagc accaacacca gccgcagcgt gcccagcccc gccaagagca
     4021 cccccaacag ctccgtgccc gcctctgctc ctgctcccag cctgacacgg tcccagctgg
     4081 acagagtgga ggccctgctg tcccccgagg acaagatcag cctgaacatg gccaagccct
     4141 tccgggagct ggaacccgag ctggtgaccc ggcggaagaa cgacttccag cggctgtaca
     4201 ccaacgaccg ggaggactac ctgggcaagc tggaacggga catcaccaag ttcttcgtgg
     4261 accggggctt cctggaaatc aagagcccca tcctgatccc cgccgagtac gtggagcgga
     4321 tgggcatcaa caacgacacc gagctgtcca agcagatttt ccgggtggac aagaacctgt
     4381 gcctgcggcc tatgctggcc cccaccctgt acaactacct gcggaaactg gacagaatcc
     4441 tgcctggccc catcaagatt ttcgaagtgg gaccctgcta ccggaaagag agcgacggca
     4501 aagagcacct ggaagagttt acaatggtgg gctttgccca gatgggcagc ggctgcaccc
     4561 gggagaacct ggaagccctg atcaaagagt tcctggatta cctggaaatc gacttcgaga
     4621 tcgtgggcga cagctgcatg gtgtacggcg acaccctgga catcatgcac ggcgacctgg
     4681 aactgagcag cgccgtggtg ggacccgtgt ccctggaccg ggagtggggc atcgacaagc
     4741 cctggatcgg agccggcttc ggcctggaac ggctgctgaa agtgatgcac ggcttcaaga
     4801 acatcaagcg ggccagcaga agcgagagct actacaacgg catcagcacc aacctgtgat
     4861 gagaattcaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4921 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4981 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5041 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5101 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5161 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5221 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5281 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5341 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5401 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5461 gccgcctccc cgcgtcgact ttaactcgag tctagagggc ccgtttaaac ccgctgatca
     5521 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5581 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5641 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5701 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5761 gcggaaagaa ccagatctcg ggcaggaaga gggcctattt cccatgattc cttcatattt
     5821 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
     5881 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     5941 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     6001 cttggcttta tatatcttgt ggaaaggacg aaacaccgga aacctgatca tgtagatcga
     6061 acggactcta aatccgttca gccgggttag attcccgggg tttccgtttt ttaatatttg
     6121 catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atccctatca gtgatagaga
     6181 cttataagtt ccctatcagt gatagagaca ccggggggtg gatcgaatag atcacacgga
     6241 ctctaaatcc gtgcaggcgg gtgaaactcc cgcacccccc gttttttcag ctggggctct
     6301 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg caagccctgc catagccact
     6361 acgggtacgt aggccaacca ctagaactat agctagagtc ctgggcgaac aaacgatgct
     6421 cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc
     6481 gccgcgacgg ccgaggtcta ccgatctcct gaagccaggg cagatccgtg cacagcacct
     6541 tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc
     6601 gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt
     6661 gacgcacacc gtggaaacgg atgaaggcac gaacccagtt gacataagcc tgttcggttc
     6721 gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     6781 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac
     6841 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     6901 tatggagcag caacgatgtt acgcagcagc aacgatgtta cgcagcaggg cagtcgccct
     6961 aaaacaaagt taggtggctc aagtatgggc atcattcgca catgtaggct cggccctgac
     7021 caagtcaaat ccatgcgggc tgctcttgat cttttcggtc gtgagttcgg agacgtagcc
     7081 acctactccc aacatcagcc ggactccgat tacctcggga acttgctccg tagtaagaca
     7141 ttcatcgcgc ttgctgcctt cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt
     7201 ctgcccaggt ttgagcagcc gcgtagtgag atctatatct atgatctcgc agtctccggc
     7261 gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac
     7321 gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct
     7381 ctctatacaa agttgggcat acgggaagaa gtgatgcact ttgatatcga cccaagtacc
     7441 gccacctaac aattcgttca agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa
     7501 ttgtcacaac gccgcgaata tagtctttac catgcccttg gccacgcccc tctttaatac
     7561 gacgggcaat ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt
     7621 atgctttttc acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag
     7681 actttattgt catagtttag atctattttg ttcagtttaa gactttattg tccgcccaca
     7741 cccgcttacg cagggcatcc atttattact caaccgtaac cgattttgcc aggttacgcg
     7801 gctggtctgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     7861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8221 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8461 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9781 aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     9841 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     9901 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     9961 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
    10021 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
    10081 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
    10141 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
    10201 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
    10261 ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa cgaagagatg
    10321 acagaaaaat tttcattctg tgacagagaa
//
