LOCUS       Exported               10484 bp ds-DNA     circular SYN 14-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Rob Hurt
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 25, 2023 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ColE1 ORI
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(742..783)
                     /label=pCMV-AnaN-V-WPRE_RB
     primer_bind     747..788
                     /label=pCMV-AnaN-V-WPRE_F1
     primer_bind     758..781
                     /label=CMV_Gib_FOR
     enhancer        778..1081
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1083..1285
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1177..1199
                     /label=CMV_Seq_F
                     /note="from RH"
     primer_bind     1236..1256
                     /label=CMVfor
     primer_bind     1251..1268
                     /label=miniCMV-F
     primer_bind     complement(1283..1306)
                     /label=CMV_Gib_REV
     misc_feature    1299
                     /label=+1
     primer_bind     complement(1362..1380)
                     /label=AnaPoly∆N-R
     primer_bind     complement(1362..1380)
                     /label=Kozak-R
     primer_bind     1366..1383
                     /label=TRE3GV-GvpN-F
     regulatory      1369..1378
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    1369..1374
                     /label=Kozak
     CDS             1375..2382
                     /codon_start=1
                     /gene="gvpN"
                     /product="Important for elongation"
                     /label=gvpN
                     /note="putative gas vesicle protein N"
                     /protein_id="AAA58711.1"
                     /translation="MVTTTKVNHKRAVLRLRPGQFVVTPAIERVAIRALRYLKSGFPVH
                     LRGPAGTGKTTLAMHLANCLDRPVMLLFGDDQFKSSDLIGSESGYTHKKVLDNYIHSVV
                     KLEDEFKQNWVDSRLTLACREGFTLVYDEFNRSRPEVNNVLLSALEEKILSLPPSSNQP
                     EYLSVNPQFRVIFTSNPEEYAGVHSTQDALMDRLVTISMPEPDEITQTEILIQKTNIDR
                     ESANFIVRLVKSFRLATGAEKTSGLRSCLMIAKVCADNNIPVTTESLDFPDIAIDILFN
                     RSHLSMSESTNIFLELLDKFSAEELEILNNRVTGDNDFLIDNSQFVSQQLAGQPN"
     misc_feature    1378..1380
                     /label=N-end rule
     primer_bind     1385..1407
                     /label=mam-afGvpN-Nterm-F
     primer_bind     complement(1434..1448)
                     /label=Mam-Ana-GvpN_R
                     /note="from RH"
     primer_bind     1631..1639
                     /label=pCMV-AnaN-V-WPRE_FB
     primer_bind     complement(1847..1855)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     complement(1965..1986)
                     /label=GvpN-269-R
     primer_bind     2039..2047
                     /label=pCMV-AnaN-V-WPRE_FB
     primer_bind     complement(2140..2161)
                     /label=GvpN-260-R
     primer_bind     complement(2163..2184)
                     /label=GvpN-269-R
     primer_bind     2187..2208
                     /label=GvpN-F270S-F
     primer_bind     complement(2223..2244)
                     /label=mam-GvpW-Trunc-R
     primer_bind     2285..2310
                     /label=Mam-Ana-GvpN_F
                     /note="from RH"
     primer_bind     complement(2358..2368)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     complement(2370..2388)
                     /label=AnaPoly∆J-R
     CDS             2383..2400
                     /codon_start=1
                     /label=linker_1
                     /translation="GAPGSG"
     primer_bind     2396..2412
                     /label=AnaPoly∆G-F
     CDS             2401..2457
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2458..3201
                     /codon_start=1
                     /label=GvpJ
                     /translation="VLPTRPQTNSSRTINTSTQGSTLADILERVLDKGIVIAGDISISI
                     ASTELVHIRIRLLISSVDKAKEMGINWWESDPYLSTKAQRLVEENQQLQHRLESLEAKL
                     NSLTSSSVKEEIPLAADVKDDLYQTSAKIPSPVDTPIEVLDFQAQSSGGTPPYVNTSME
                     ILDFQAQTSAESSSPVGSTVEILDFQAQTSEESSSPVVSTVEILDFQAQTSEESSSPVG
                     STVEILDFQAQTSEEIPSSVDPAIDV"
     primer_bind     2464..2484
                     /label=AnaPoly∆N-F
     primer_bind     complement(2551..2570)
                     /label=Mam-Ana-GvpJ_R
                     /note="from RH"
     primer_bind     complement(2727..2734)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     complement(2780..2789)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     3001..3013
                     /label=Mam-Ana-GvpJ_F
                     /note="from RH"
     primer_bind     3063..3076
                     /label=Mam-Ana-GvpJ_F
                     /note="from RH"
     primer_bind     3122..3139
                     /label=Mam-Ana-GvpJ_F
                     /note="from RH"
     CDS             3202..3219
                     /codon_start=1
                     /label=Linker_2
                     /translation="GAPGSG"
     primer_bind     3208..3233
                     /label=AnaPoly∆J-F
     CDS             3220..3276
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_2
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3277..3741
                     /codon_start=1
                     /label=GvpK
                     /translation="VVCTPAENFNNSLTIASKPKNEAGLAPLLLTVLELVRQLMEAQVI
                     RRMEEDLLSEPDLERAADSLQKLEEQILHLCEMFEVDPADLNINLGEIGTLLPSSGSYY
                     PGQPSSRPSVLELLDRLLNTGIVVDGEIDLGIAQIDLIHAKLRLVLTSKPI"
     primer_bind     complement(3323..3342)
                     /label=Mam-Ana-GvpK_R
                     /note="from RH"
     primer_bind     3664..3684
                     /label=Mam-Ana-GvpK_F
                     /note="from RH"
     primer_bind     complement(3729..3747)
                     /label=AnaPoly∆F-R
     CDS             3742..3759
                     /codon_start=1
                     /label=Linker_3
                     /translation="GAPGSG"
     CDS             3760..3816
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_3
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3817..4551
                     /codon_start=1
                     /label=GvpF
                     /translation="VSIPLYLYGIFPNTIPETLELEGLDKQPVHSQVVDEFCFLYSEAR
                     QEKYLASRRNLLTHEKVLEQTMHAGFRVLLPLRFGLVVKDWETIMSQLINPHKDQLNQL
                     FQKLAGKREVSIKIFWDAKAELQTMMESHQDLKQQRDNMEGKKLSMEEVIQIGQLIEIN
                     LLARKQAVIEVFSQELNPFAQEIVVSDPMTEEMIYNAAFLIPWESESEFSERVEVIDQK
                     FGDRLRIRYNNFTAPYTFAQLDS"
     primer_bind     complement(3889..3901)
                     /label=Mam-Ana-GvpF_R
                     /note="from RH"
     primer_bind     complement(3930..3942)
                     /label=Mam-Ana-GvpG_F
                     /note="from RH"
     primer_bind     complement(4377..4392)
                     /label=AnaPoly∆N-R
     primer_bind     complement(4377..4392)
                     /label=Kozak-R
     primer_bind     4439..4447
                     /label=pCMV-AnaN-V-WPRE_FB
     primer_bind     4440..4455
                     /label=Mam-Ana-GvpF_F
                     /note="from RH"
     primer_bind     complement(4538..4557)
                     /label=AnaPoly∆G-R
     CDS             4552..4569
                     /codon_start=1
                     /label=Linker_4
                     /translation="GAPGSG"
     primer_bind     4558..4576
                     /label=AnaPoly∆F-F
     CDS             4570..4626
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_4
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4627..4983
                     /codon_start=1
                     /label=GvpG
                     /translation="VLTKLLLLPIMGPLNGVVWIAEQIQERTNTEFDAQENLHKQLLSL
                     QLSFDIGEIGEEEFEIQEEEILLKIQALEEEARLELEAEQEEARLELEAEQEDFEYPPQ
                     FTAEVNKDQHLVLLP"
     primer_bind     complement(4701..4719)
                     /label=Mam-Ana-GvpG_R
                     /note="from RH"
     primer_bind     complement(4856..4870)
                     /label=AnaPoly∆G-R
     primer_bind     4862..4906
                     /label=Mam-Ana-GvpG_F
                     /note="from RH"
     primer_bind     4895..4918
                     /label=Mam-Ana-GvpG_F
                     /note="from RH"
     CDS             4984..5001
                     /codon_start=1
                     /label=Linker_2
                     /translation="GAPGSG"
     primer_bind     4990..5013
                     /label=AnaPoly∆G-F
     CDS             5002..5058
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5059..5736
                     /codon_start=1
                     /label=GvpW
                     /translation="VELENLYTYAFLEIPSSPLILPQGAANQVVLINGTELAAIVEPGI
                     FLESFQNNDEKIIQMALSHDRVICELFQQITVLPLRFGTYFTSTNNLLNHLKSHEKEYQ
                     NKLEKINGKNEFTLKLIPRMIEEIVPSEGGGKDYFLAKKQRYQNQNNFSIAQAAEKQNL
                     IDLITKVNQLPVVVQEQEEQIQIYLLVSCQDKTLLLEQFLTWQKACPKWDLLLGDCLPP
                     YHFI"
     primer_bind     complement(5102..5109)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     complement(5151..5169)
                     /label=Mam-Ana-GvpW_R
                     /note="from RH"
     primer_bind     complement(5340..5361)
                     /label=mam-GvpW-Trunc-R
     primer_bind     complement(5385..5393)
                     /label=Mam-Ana-GvpW_R
                     /note="from RH"
     primer_bind     5386..5394
                     /label=CMV_Seq_F
                     /note="from RH"
     primer_bind     complement(5452..5463)
                     /label=pCMV-AnaN-V-WPRE_R1
     primer_bind     5593..5602
                     /label=Mam-Ana-GvpG_F
                     /note="from RH"
     primer_bind     5636..5656
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     5698..5707
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     complement(5723..5753)
                     /label=miniCMV-F
     CDS             5737..5754
                     /codon_start=1
                     /label=linker_1
                     /translation="GAPGSG"
     primer_bind     5750..5768
                     /label=AnaPoly∆J-F
     CDS             5755..5811
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_2
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5812..6153
                     /codon_start=1
                     /label=GvpV
                     /translation="VIKNIQVFFMKTISNRSISRAKISTMPRPKSDASSQLDLYKMVTE
                     KQRIQRDMYSIKERMGLLQQRLDILNQQIEATEKTIHKLRQPHSNTAQNIVRSNIFVES
                     NNYQTFEVEY"
     primer_bind     complement(5909..5916)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     primer_bind     complement(6000..6007)
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     CDS             6154..6171
                     /codon_start=1
                     /label=Linker_3
                     /translation="GAPGSG"
     primer_bind     6163..6178
                     /label=AnaPoly∆F-F
     CDS             6172..6228
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_4
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6229..6948
                     /codon_start=1
                     /label=EmGFP
                     /translation="VVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     6230..6250
                     /label=EmGFP-F
     primer_bind     6313..6331
                     /label=pCMV-AnaN-V-WPRE_FB
     primer_bind     complement(6325..6342)
                     /label=pCMV-AnaN-V-WPRE_R1
     primer_bind     complement(6344..6361)
                     /label=emGFP-R
                     /note="from RH"
     primer_bind     complement(6411..6419)
                     /label=GvpN-269-R
     primer_bind     complement(6513..6523)
                     /label=Mam-Ana-GvpG_R
                     /note="from RH"
     primer_bind     6573..6580
                     /label=pCMV-AnaN-V-WPRE_FB
     primer_bind     complement(6694..6702)
                     /label=Mam-Ana-GvpF_R
                     /note="from RH"
     primer_bind     6887..6895
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     misc_feature    6967..6974
                     /label=random
     primer_bind     6975..7000
                     /label=pCMV_Sport-fwd
     primer_bind     6975..6998
                     /label=mam-GvpW-Trunc-F
     primer_bind     complement(7036..7055)
                     /label=T7 Promoter
     promoter        complement(7037..7055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7308..7315)
                     /label=pCMV_Sport-fwd
     primer_bind     complement(7387..7394)
                     /label=miniCMV-F
     misc_feature    7442..7449
                     /label=random
     misc_feature    7450..8038
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     primer_bind     7561..7572
                     /label=emGFP-R
                     /note="from RH"
     primer_bind     complement(7631..7648)
                     /label=WPREseqR
                     /note="from RH"
     primer_bind     7784..7794
                     /label=Mam-Ana-GvpW_F
                     /note="from RH"
     CDS             complement(7921..7932)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7989..8002
                     /label=pCMV-AnaN-V-WPRE_R1
     primer_bind     complement(7990..7999)
                     /label=pCMV-AnaN-V-WPRE_FB
     misc_feature    8038
                     /label=end of lenti insert
     misc_feature    8039..8057
                     /label=random
     polyA_signal    8058..8534
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8521..8544)
                     /label=hGH-polyA-R
     primer_bind     complement(8521..8544)
                     /label=hGHpA-inv-R
     misc_feature    8535..8544
                     /label=random
     rep_origin      8669..9124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             9412..10272
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    complement(10374..10407)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttattcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc ccatatatgg agttccgcgt
      781 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      841 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      901 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      961 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1021 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1081 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1141 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1201 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1261 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcagat ctttgtcgat
     1321 cctaccatcc actcgacaca cccgccagcg gccgctgcga gggtctccgc caccatggtg
     1381 accaccacca aagtgaacca caagcgggcc gtgctgagac tgaggcctgg ccagtttgtc
     1441 gtgacccccg ccattgagcg ggtggccatt agagccctga gatacctgaa gtccggcttc
     1501 cccgtgcacc tgagaggacc tgccggaacc ggcaagacca cactggccat gcacctggcc
     1561 aactgcctgg acagacccgt gatgctgctg ttcggcgacg accagttcaa gagcagcgac
     1621 ctgatcggca gcgagagcgg ctacacccac aagaaggtgc tggacaacta catccacagc
     1681 gtcgtgaagc tggaagatga gttcaagcag aactgggtgg acagcagact gaccctggcc
     1741 tgccgggaag gcttcaccct ggtgtacgac gagttcaacc ggtccagacc cgaagtgaac
     1801 aacgtgctgc tgagcgccct ggaagagaag atcctgagcc tgccccccag cagcaaccag
     1861 ccagagtacc tgagcgtgaa cccccagttc agagtgatct tcaccagcaa ccccgaggaa
     1921 tatgccggcg tgcacagcac ccaggacgcc ctgatggacc ggctcgtgac catctccatg
     1981 cccgagcccg atgagatcac ccagaccgag atcctgatcc agaaaacaaa catcgaccgc
     2041 gagagcgcca acttcatcgt gcggctcgtg aagtccttca gactggccac aggcgccgag
     2101 aaaaccagcg gcctgagaag ctgcctgatg atcgccaaag tgtgcgccga caacaacatc
     2161 cccgtgacca ccgagagcct ggacttcccc gatatcgcca tcgacatcct gttcaaccgc
     2221 agccacctga gcatgagcga gtccaccaac atctttctgg aactgctgga taagttcagc
     2281 gccgaggaac tggaaatcct gaacaacaga gtgaccggcg acaacgactt cctgatcgac
     2341 aacagccagt tcgtgtccca gcagctggcc ggacagccca acggagcgcc aggttccggg
     2401 gctactaact tcagcctcct taaacaggct ggcgatgtgg aagaaaatcc cggaccagtg
     2461 ctgcccacca gaccccagac caacagcagc cggaccatca acaccagcac ccagggcagc
     2521 accctggccg acatcctgga aagagtgctg gacaagggca tcgtgatcgc cggggacatc
     2581 agcatctcta tcgccagcac cgagctggtg cacatccgga tcagactgct gatctccagc
     2641 gtggacaagg ccaaagagat gggcatcaac tggtgggaga gcgaccccta cctgagcacc
     2701 aaggcccaga gactggtgga agagaaccag cagctccagc acagactgga aagcctggaa
     2761 gccaagctga acagcctgac cagcagcagc gtgaaagagg aaatccccct ggccgccgac
     2821 gtgaaggacg acctgtatca gaccagcgcc aagatcccca gccccgtgga tacccctatc
     2881 gaggtgctgg acttccaggc ccagtctagc ggcggcacac ccccttacgt gaacacctct
     2941 atggaaatac tggattttca ggctcagacc tccgccgaga gcagcagccc tgtgggctct
     3001 accgtggaaa tactggactt ccaggcacag acaagcgagg aatccagctc ccccgtggtg
     3061 tccacagtgg aaatactgga tttccaggcc cagacttctg aagagtccag ctctccagtg
     3121 ggaagcactg tggaaatact ggactttcag gcccagacca gtgaagagat ccccagctct
     3181 gtggaccccg ccatcgatgt gggtgccccg ggatctggcg caacaaattt tagtctcctc
     3241 aagcaggcag gagatgtcga ggaaaaccct ggacccgtgg tgtgcacccc tgccgagaac
     3301 ttcaacaaca gcctgacaat cgccagcaag cccaagaacg aggccggact ggctcctctg
     3361 ctgctgaccg tgctggaact cgtgcggcag ctgatggaag cccaagtgat ccggcggatg
     3421 gaagaggacc tgctgagcga gcccgacctg gaaagagccg ccgatagcct ccagaagctg
     3481 gaagaacaga tcctgcacct gtgcgagatg ttcgaggtgg accccgccga cctgaacatc
     3541 aacctgggcg agatcggcac cctgctgcct agcagcggca gctactatcc tggccagcca
     3601 agcagcagac cctctgtgct ggaactgctg gaccggctgc tgaacaccgg catcgtggtg
     3661 gacggcgaga ttgacctggg aatcgcccag atcgacctga tccacgccaa gctgagactg
     3721 gtgctgacct ccaagcccat cggggcacct ggctcaggag cgaccaactt ctcattgctc
     3781 aaacaagccg gagatgttga ggagaatcca ggccctgtga gcatccccct gtacctgtac
     3841 ggcatcttcc ccaacaccat ccccgagaca ctggaactgg aaggcctgga caagcagccc
     3901 gtgcacagcc aggtggtgga cgagttctgc ttcctgtaca gcgaggcccg gcaggaaaag
     3961 tacctggcca gcagacggaa cctgctgacc cacgagaagg tgctggaaca gaccatgcac
     4021 gccggcttca gagtgctgct gcccctgaga tttggcctgg tcgtgaagga ctgggagaca
     4081 atcatgagcc agctgatcaa cccccacaag gaccagctga accagctgtt ccagaagctg
     4141 gccggcaagc gggaagtgtc catcaagatt ttctgggacg ccaaggccga gcttcagacc
     4201 atgatggaaa gccaccagga cctgaagcag cagcgggaca acatggaagg caagaaactg
     4261 agcatggaag aagtgatcca gatcgggcag ctgatcgaga tcaacctgct ggcccggaag
     4321 caggccgtga tcgaggtgtt cagccaggaa ctgaacccct tcgcccagga aatcgtggtg
     4381 tccgacccca tgaccgagga aatgatctac aacgccgcct tcctgatccc ctgggagagc
     4441 gagagcgagt tcagcgagcg ggtggaagtg atcgaccaga agttcggcga ccggctgcgg
     4501 atcagataca acaacttcac cgccccctac acctttgccc agctggactc tggcgctccc
     4561 gggtcaggtg ccactaattt ttcattgttg aagcaagctg gggatgttga agagaaccca
     4621 gggcctgtgc tgaccaaact cctgctgctg cccatcatgg gacccctgaa tggcgtcgtg
     4681 tggatcgccg agcagatcca ggaacggacc aacaccgagt tcgacgccca ggaaaacctg
     4741 cacaaacagc tgctgagcct ccagctgagc ttcgacatcg gcgagatcgg agaggaagag
     4801 ttcgagattc aggaagagga aatcctgctg aagatccagg ccctggaaga agaggcccgg
     4861 ctggaactgg aagccgagca ggaagaagcc agactggaac tggaagctga acaggaagat
     4921 ttcgagtacc caccacagtt caccgccgaa gtgaacaagg atcagcatct ggtgctgctg
     4981 cccggtgccc cgggatctgg cgctactaac ttcagcctcc ttaaacaggc tggcgatgtg
     5041 gaagaaaatc ccggaccagt ggaactggaa aacctgtaca cctacgcctt tctggaaatc
     5101 cccagcagcc ccctgatcct gcctcagggc gctgctaatc aggtggtgct gatcaacggc
     5161 accgagctgg ccgccatcgt ggaacctggc atcttcctgg aatccttcca gaacaacgac
     5221 gagaagatca tccagatggc cctgagccac gacagagtga tctgcgagct gttccagcag
     5281 atcaccgtgc tgcccctgag attcggcacc tacttcacca gcaccaacaa cctgctgaac
     5341 cacctgaagt cccacgagaa agagtaccag aacaagctgg aaaagatcaa cgggaagaac
     5401 gagttcaccc tgaagctgat cccccggatg atcgaggaaa tcgtgccctc tgagggcgga
     5461 ggcaaggact acttcctggc caagaagcag cgctaccaga atcagaacaa cttctctatc
     5521 gcccaggccg ccgagaagca gaacctgatc gacctgatca ccaaagtgaa ccagctgccc
     5581 gtggtggtgc aggaacagga agaacagatc caaatctacc tgctggtgtc ctgccaggat
     5641 aagaccctgc tgctggaaca gttcctgacc tggcagaaag cctgccccaa gtgggatctg
     5701 ctgctgggcg actgcctgcc cccctaccac tttatcggag cgccaggttc cggggcaaca
     5761 aattttagtc tcctcaagca ggcaggagat gtcgaggaaa accctggacc cgtgatcaag
     5821 aacatccagg tgttctttat gaaaaccatc agcaaccggt ccatcagccg ggccaagatc
     5881 agcaccatgc ccagacctaa gagcgacgcc agcagccagc tggacctgta caagatggtc
     5941 accgagaagc agagaatcca gcgggatatg tacagcatca aagaacggat gggcctgctc
     6001 cagcagcggc tggacatcct gaaccagcag atcgaggcca ccgagaaaac catccacaag
     6061 ctgcggcagc cccacagcaa caccgcccag aatatcgtgc ggagcaacat cttcgtggaa
     6121 agcaacaact accagacctt cgaggtggaa tacggggcac ctggctcagg agccactaat
     6181 ttttcattgt tgaagcaagc tggggatgtt gaagagaacc cagggcctgt ggtgtccaag
     6241 ggcgaggaac tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac
     6301 ggccacaagt tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc
     6361 ctgaagttca tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca
     6421 ctgacctacg gcgtgcagtg cttcgccaga taccccgacc acatgaagca gcacgatttc
     6481 ttcaagagcg ccatgcccga gggctacgtg caggaacgga ccatcttctt caaggacgac
     6541 ggcaactaca agacaagagc cgaagtgaag ttcgagggcg acaccctcgt gaaccggatc
     6601 gagctgaagg gcatcgactt caaagaggat ggcaacatcc tgggccacaa gctggagtac
     6661 aactacaaca gccacaaggt gtacatcacc gccgacaagc agaaaaacgg catcaaagtg
     6721 aacttcaaga cccggcacaa catcgaggac ggcagcgtgc agctggccga ccactaccag
     6781 cagaacaccc ccatcggaga tggccccgtg ctgctgcccg acaaccacta cctgagcaca
     6841 caaagcgccc tgagcaagga ccccaacgag aagcgggacc acatggtgct gctggaattt
     6901 gtgaccgccg ctggcatcac cctgggcatg gacgagctgt acaagtgact cgagtctaga
     6961 gggccccgtg gctgtaatct agaggatccc tcgaggggcc caagcttacg cgtgcatgcg
     7021 acgtcatagc tctctcccta tagtgagtcg tattataagc tagcttggga tctttgtgaa
     7081 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     7141 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctagctgcat atgcttgctg
     7201 cttgagagtt ttgcttactg agtatgattt atgaaaatat tatacacagg agctagtgat
     7261 tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc ccctcagtcc
     7321 tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7381 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7441 tcgtggctga atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7501 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7561 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7621 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7681 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7741 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7801 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7861 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7921 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7981 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcca
     8041 gagacaatta acttcgcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     8101 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     8161 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     8221 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     8281 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     8341 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     8401 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     8461 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     8521 ccttccctgt ccttgaagtt ctcagatcct gcattaatga atcggccaac gcgcggggag
     8581 aggcggtttg cgtattggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8641 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     8701 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8761 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8821 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8881 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8941 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9001 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9061 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     9121 atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9181 atacattcaa atatgtatcc gctcatgcca ggtcttggac tggtgagaac ggcttgctcg
     9241 gcagcttcga tgtgtgctgg agggagaata aaggtctaag atgtgcgata gagggaagtc
     9301 gcattgaatt atgtgctgtg tagggatcgc tggtatcaaa tatgtgtgcc cacccctggc
     9361 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9421 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9481 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9541 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9601 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9661 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9721 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9781 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9841 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9901 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9961 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10021 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10081 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10141 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10201 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10261 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10321 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gccataactt
    10381 cgtataatgt atgctatacg aagttatggc atgaccaaaa tcccttaacg tgagttttcg
    10441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
