LOCUS       pBBR1_DVK_AE(J23106-B00 3631 bp ds-DNA     circular     25-JAN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5..39
                     /label="J23106"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    44..63
                     /label="B0033m"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             68..787
                     /label="E0040m"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      792..920
                     /label="B0015"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    925..932
                     /label="BbsI RS rev AT spacer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    933..937
                     /label="spacer1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    938..966
                     /label="spacer 6"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    967..974
                     /label="AscI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    975..1061
                     /label="spacer 5"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1062..1833
                     /label="pBBR1 oriV"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1834..2496
                     /label="pBBR1 Rep"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2497..2504
                     /label="FseI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2505..2615
                     /label="partial oriT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2616..2635
                     /label="spacer 9"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2636..3602
                     /label="KanR cassette"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3603..3623
                     /label="spacer3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3624..3631
                     /label="BbsI RS fwd AT spacer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 GGAGtttacg gctagctcag tcctaggtat agtgctagct actagagtca cacaggacta
       61 ctaaatgatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      121 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      181 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      241 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      301 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      361 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      421 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      481 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      541 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      601 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      661 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagatcacat
      721 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
      781 ataataaAGG Tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      901 tgggcctttc tgcgtttata GCTTatgtct tcagcagtag gacaaatccg ccgccctaga
      961 cagctgggcg cgccccccct acgggcttgc tctccgggct tcgccctgcg cggtcgctgc
     1021 gctcccttgc cagcccgtgg atatgtggac gatggccgcg agcggccacc ggctggctcg
     1081 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
     1141 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
     1201 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
     1261 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
     1321 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
     1381 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
     1441 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
     1501 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
     1561 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
     1621 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
     1681 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
     1741 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
     1801 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
     1861 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
     1921 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1981 cagatgggcc accagaacgc cgtggtggtC AGCCAGAAAA CACTTTCCAa gctcatcgga
     2041 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     2101 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     2161 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     2221 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     2281 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     2341 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc
     2401 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     2461 gagccgccga cacgggtaac gctgccgcgc cggtagggcc ggcctacggc cagcctcgca
     2521 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     2581 cgctcgcggg tgggcctact tcacctatcc tgcccTTGTG TCTCAAAATC TCTGAtgtac
     2641 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2701 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta
     2761 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2821 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2881 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2941 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     3001 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
     3061 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3121 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     3181 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3241 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     3301 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3361 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3421 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3481 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta aggtaactgt
     3541 cagaccaagt ttactcatat atactttaga ttgatttttc gttCCACTGA GCGTCAGACC
     3601 CCAGAACTCT GTCAGACCga gtggaagaca t
//