LOCUS       vYM013_phiC31-nTRE-InsM 6454 bp ds-DNA     circular     18-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..683
                     /label="ColE1 origin"
     misc_feature    1229..1501
                     /label="phiC31_attB"
     misc_feature    1573..2373
                     /label="NeoR"
     primer          complement(1589..1609)
                     /label="oYM073"
                     /note="sequence: CTGCGTGCAATCCATCTTGTT"
     misc_feature    2390..2666
                     /label="BGH/pA"
     primer          2404..2428
                     /label="oYM194"
                     /note="sequence: acctgagcctcgactgtgccttctagtt"
     primer          2694..2718
                     /label="oYM076"
                     /note="sequence: atagcactgcagtttactccctatc"
     misc_feature    2711..2729
                     /label="TRE"
     misc_feature    2747..2765
                     /label="TRE"
     misc_feature    2783..2801
                     /label="TRE"
     misc_feature    2819..2837
                     /label="TRE"
     misc_feature    2855..2873
                     /label="TRE"
     primer          2862..2885
                     /label="oYM124"
                     /note="sequence: cagtgatagagaagctatgtgcag"
     primer          2870..2907
                     /label="oYM380"
                     /note="sequence: gagaagTtatgtgTaggtagggTtgtaTagtgggaatg"
     primer          2876..2907
                     /label="oYM362"
                     /note="sequence: TtatgtgTaggtagggTtgtaTagtgggaatg"
     misc_feature    2914..3214
                     /label="CpG_Insert_Max"
     primer          2916..2937
                     /label="oYM435"
                     /note="sequence: GTCGTATACTATACGCGTTCGC"
     primer          complement(3051..3070)
                     /label="oYM436"
                     /note="sequence: GCGTGCAACGAAATAAACGT"
     primer          3219..3264
                     /label="oYM146"
                     /note="sequence: gagaagacctcgtaggatcggcgatcgctccggtgcccgtcagcgggcagagcgcacatc"
     promoter        3219..3762
                     /label="pEF1s"
     misc_feature    3254..3460
                     /label="pEF Homo"
     primer          complement(3332..3368)
                     /label="oYM381"
                     /note="sequence: RaaaccAATACACRACATCACTTTCCCAATTTACCCC"
     misc_feature    3471..3745
                     /label="T-Virus Homo"
     primer          complement(3720..3768)
                     /label="oYM151"
                     /note="sequence: tcgaagacccaggtgtaggcccaggtcacagcttggatctgtaacggcgcagaacag"
     primer          complement(3810..3859)
                     /label="oYM364"
                     /note="sequence: caaaaRaaaaacaaacttcRctAActctAAcatAAtAAcaaActtAatat"
     misc_feature    3821..3826
                     /label="Kozak"
     misc_feature    3827..4204
                     /label="H2B"
     misc_feature    4229..4945
                     /label="Citrine"
     primer          complement(4924..4946)
                     /label="oYM355"
                     /note="sequence: aGGTCTCgAGGTTCActtgtacagctcgtccatgccg"
     misc_feature    5028..5411
                     /label="SV40/pA"
     primer          complement(5315..5370)
                     /label="oYM276"
                     /note="sequence: GCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAA"
     misc_feature    5698..6333
                     /label="AmpR"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcggta ccgatcataa tcagccatac cacatttgta gaggttttac
      781 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      841 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      961 atgtatctta tcatgtctgg atcataatca gccataccac atttgtagag gttttacttg
     1021 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1081 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1201 tatcttatca tgtctgctcg aggtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg
     1261 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca
     1321 tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga
     1381 agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg
     1441 cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca
     1501 tgtcgacggt atcgataagc ttacgcgtgc tagcgcatgc agatctacta gtcggccgga
     1561 tatcaactcg agatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     1621 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     1681 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     1741 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     1801 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     1861 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     1921 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     1981 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2041 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2101 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     2161 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     2221 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     2281 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     2341 atcgccttct atcgccttct tgacgagttc ttctgagggg atcaattctc tagagctcgc
     2401 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2461 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2521 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2581 aagggggagg attgggaaga gaatagcagg catgctgggg atgcggtggg ctctatggct
     2641 tctgaggcgg aaagaaccag ctgggggcta gcaaagccta ggaacagctg catatagcac
     2701 tgcagtttac tccctatcag tgatagagag tcaaaaggcc atgccctccc tatcagtgat
     2761 agagagagca gactcaaggt ggtccctatc agtgatagag agattgagca agagggcctc
     2821 cctatcagtg atagagattg atccagtcag ggcctcccta tcagtgatag agaagctatg
     2881 tgcaggtagg gctgtacagt gggaatgcaC GTAACGTCGT ATACTATACG CGTTCGCGTT
     2941 CACGACGAAC GAAAACGACG GCGACGCGCG ACGTCGCGAA CGCGCGCGCG CCGTCGGACG
     3001 CGGCGCGCTC GTCCGAATTG CGCGCGCGCG CGTAACGACG TTCGTATCGA ACGTTTATTT
     3061 CGTTGCACGC GCGCGTTCGA CGCCTAACGA CGACGCGAAC CGCGCGAAAC GCGCGTTACG
     3121 CGCGATTTCG AGTCGACGCG CGGCGTCGCG CGTCGCCGGC GCGCGACGAT CGCGGTACGC
     3181 GCGCACGTCG GCGCGTACGT ACGCGTATCG CGTTgtaggg atcggcgatc gctccggtgc
     3241 ccgtcagcgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     3301 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     3361 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3421 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     3481 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     3541 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     3601 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     3661 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     3721 tgttctgcgc cgttacagat ccaagctgtg acctgggcct acACCTGGCC gctctagccc
     3781 gggcggatcc cccgggctgc aggaattcga tatcaagctt gccaccatgc cagagccagc
     3841 gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa
     3901 aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt
     3961 tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc
     4021 gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa
     4081 caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg
     4141 ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc
     4201 taaggatccc cgggtaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     4261 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4321 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4381 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg
     4441 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4501 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4561 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4621 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4681 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4741 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4801 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     4861 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4921 tctcggcatg gacgagctgt acaagtaggt cgacctcgag ggggggcccg gtaccaggta
     4981 agtgtaccca attcgcccta tagtgagtcg tattacaatt cactcgatcg cccttcccaa
     5041 cagttgcgca gcctgaatgg cgaatggaga tccaattttt aagtgtataa tgtgttaaac
     5101 tactgattct aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc
     5161 tcagtcctca cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac
     5221 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5281 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5401 atgtatctta acgcgtaaat tgtaagcgtc tcaaatatgt atccgctcat gagacaataa
     5461 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5521 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5581 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5641 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5701 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5761 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5821 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5881 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5941 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6001 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6061 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6181 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6421 aaaaggatct aggtgaagat cctttttgat aatc
//