LOCUS       vYM141_phiC31_nTRE_pEF1 6144 bp ds-DNA     circular     13-FEB-2020
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
FEATURES             Location/Qualifiers
     rep_origin      1..683
                     /label="ColE1 origin"
     misc_feature    1229..1501
                     /label="phiC31_attB"
     misc_feature    1573..2373
                     /label="NeoR"
     primer          1576..1591
                     /label="oYM134"
                     /note="sequence: cacggtctcgaagccgaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccggatcggccattgaac"
     misc_feature    2390..2666
                     /label="BGH/pA"
     primer          complement(2393..2409)
                     /label="oYM135"
                     /note="sequence: ggctgatcagcgagctc"
     primer          2404..2428
                     /label="oYM194"
                     /note="sequence: acctgagcctcgactgtgccttctagtt"
     primer          2656..2677
                     /label="oYM477"
                     /note="sequence: accagctgggggctagcaaagc"
     primer          2694..2718
                     /label="oYM076"
                     /note="sequence: atagcactgcagtttactccctatc"
     misc_feature    2711..2729
                     /label="TRE"
     misc_feature    2747..2765
                     /label="TRE"
     misc_feature    2783..2801
                     /label="TRE"
     misc_feature    2819..2837
                     /label="TRE"
     misc_feature    2855..2873
                     /label="TRE"
     primer          2903..2927
                     /label="oYM087"
                     /note="sequence: acgtATGCATggatctgcgatcgctcc"
     primer          2911..2956
                     /label="oYM146"
                     /note="sequence: gagaagacctcgtaggatcggcgatcgctccggtgcccgtcagcgggcagagcgcacatc"
     misc_feature    2911..3454
                     /label="pEF1s"
     primer          3088..3126
                     /label="oYM471"
                     /note="sequence: cgtatataagtgcagtagtcgccctgaacgttctttttc"
     primer          complement(3088..3126)
                     /label="oYM472"
                     /note="sequence: gaaaaagaacgttcagggcgactactgcacttatatacg"
     misc_feature    3090..3096
                     /label="TATA box"
     misc_feature    3110..3111
                     /label="*"
     misc_feature    3121..3137
                     /label="TSS"
     primer          complement(3412..3456)
                     /label="oYM151"
                     /note="sequence: tcgaagacccaggtgtaggcccaggtcacagcttggatctgtaacggcgcagaacag"
     primer          complement(3440..3464)
                     /label="oYM088"
                     /note="sequence: acgtGCGGCCGCgtaggcgccggtcac"
     primer          complement(3499..3519)
                     /label="oYM204"
                     /note="sequence: catggtggcaagcttgatatc"
     misc_feature    3511..3516
                     /label="Kozak"
     misc_feature    3517..3894
                     /label="H2B"
     primer          complement(3899..3920)
                     /label="oYM478"
                     /note="sequence: atggtggcgaccggtacccggg"
     misc_feature    3919..4635
                     /label="Citrine"
     primer          complement(4417..4436)
                     /label="oYM187"
                     /note="sequence: TCGATGTTGTGGCGGATCTT"
     misc_feature    4718..5101
                     /label="SV40/pA"
     misc_feature    5388..6023
                     /label="AmpR"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcggta ccgatcataa tcagccatac cacatttgta gaggttttac
      781 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      841 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      961 atgtatctta tcatgtctgg atcataatca gccataccac atttgtagag gttttacttg
     1021 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1081 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1201 tatcttatca tgtctgctcg aggtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg
     1261 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca
     1321 tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga
     1381 agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg
     1441 cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca
     1501 tgtcgacggt atcgataagc ttacgcgtgc tagcgcatgc agatctacta gtcggccgga
     1561 tatcaactcg agatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     1621 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     1681 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     1741 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     1801 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     1861 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     1921 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     1981 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2041 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2101 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     2161 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     2221 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     2281 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     2341 atcgccttct atcgccttct tgacgagttc ttctgagggg atcaattctc tagagctcgc
     2401 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2461 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2521 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2581 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2641 tctgaggcgg aaagaaccag ctgggggcta gcaaagccta ggaacagctg catatagcac
     2701 tgcagtttac tccctatcag tgatagagag tcaaaaggcc atgccctccc tatcagtgat
     2761 agagagagca gactcaaggt ggtccctatc agtgatagag agattgagca agagggcctc
     2821 cctatcagtg atagagattg atccagtcag ggcctcccta tcagtgatag agaagctatg
     2881 tgcaggtagg gctgtacagt gggaatgcat ggatctgcga tcgctccggt gcccgtcagt
     2941 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     3001 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     3061 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc ctgaacgttc
     3121 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     3181 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     3241 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     3301 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     3361 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     3421 gccgttacag atccaagctg tgaccggcgc ctacgcggcc gctctagccc gggcggatcc
     3481 cccgggctgc aggaattcga tatcaagctt gccaccatgc cagagccagc gaagtctgct
     3541 cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag
     3601 aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag
     3661 gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac
     3721 gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg
     3781 accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc
     3841 aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc taaggatccc
     3901 cgggtaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3961 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4021 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4081 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
     4141 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4201 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4261 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4321 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4381 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4441 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4501 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     4561 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4621 gacgagctgt acaagtaggt cgacctcgag ggggggcccg gtaccaggta agtgtaccca
     4681 attcgcccta tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca
     4741 gcctgaatgg cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct
     4801 aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca
     4861 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4921 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4981 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5101 acgcgtaaat tgtaagcgtc tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5401 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5701 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6121 aggtgaagat cctttttgat aatc
//