LOCUS       vYM143_phiC31-nTRE-pEF1 6149 bp ds-DNA     circular     13-FEB-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..683
                     /label="ColE1 origin"
     misc_feature    1229..1501
                     /label="phiC31_attB"
     misc_feature    1573..2373
                     /label="NeoR"
     misc_feature    2390..2666
                     /label="BGH/pA"
     primer          2656..2677
                     /label="oYM477"
                     /note="sequence: accagctgggggctagcaaagc"
     primer          2694..2718
                     /label="oYM076"
                     /note="sequence: atagcactgcagtttactccctatc"
     misc_feature    2711..2729
                     /label="TRE"
     misc_feature    2747..2765
                     /label="TRE"
     misc_feature    2783..2801
                     /label="TRE"
     misc_feature    2819..2837
                     /label="TRE"
     misc_feature    2855..2873
                     /label="TRE"
     primer          2908..2959
                     /label="oYM150"
                     /note="sequence: gagaagacctcgtaggatctgccattgctccagtgcccttcagtgggcagagagcacatc"
     primer          2914..2934
                     /label="oYM188"
                     /note="sequence: ggcctccacggccagggatctgccattgctccagtg"
     promoter        2914..3457
                     /label="pEF1s"
     misc_feature    2925..3155
                     /label="pEF Homo"
     misc_feature    2940..3433
                     /label="gYM10_pEF1s(min)"
     primer          3091..3128
                     /label="oYM475"
                     /note="sequence: catatataagtgcagtagtccccgtgaacgttcttttt"
     primer          complement(3091..3128)
                     /label="oYM476"
                     /note="sequence: aaaaagaacgttcacggggactactgcacttatatatg"
     misc_feature    3093..3099
                     /label="TATA box"
     misc_feature    3166..3440
                     /label="T-Virus Homo"
     primer          complement(3415..3463)
                     /label="oYM151"
                     /note="sequence: tcgaagacccaggtgtaggcccaggtcacagcttggatctgtaacggcgcagaacag"
     primer          complement(3448..3467)
                     /label="oYM189"
                     /note="sequence: ccttgctcaccatggtggcGGCCAGGTgtaggcccag"
     misc_feature    3516..3521
                     /label="Kozak"
     misc_feature    3522..3899
                     /label="H2B"
     primer          complement(3904..3925)
                     /label="oYM478"
                     /note="sequence: atggtggcgaccggtacccggg"
     misc_feature    3924..4640
                     /label="Citrine"
     misc_feature    4723..5106
                     /label="SV40/pA"
     misc_feature    5393..6028
                     /label="AmpR"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcggta ccgatcataa tcagccatac cacatttgta gaggttttac
      781 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      841 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      961 atgtatctta tcatgtctgg atcataatca gccataccac atttgtagag gttttacttg
     1021 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1081 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1201 tatcttatca tgtctgctcg aggtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg
     1261 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca
     1321 tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga
     1381 agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg
     1441 cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca
     1501 tgtcgacggt atcgataagc ttacgcgtgc tagcgcatgc agatctacta gtcggccgga
     1561 tatcaactcg agatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     1621 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     1681 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     1741 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     1801 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     1861 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     1921 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     1981 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2041 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2101 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     2161 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     2221 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     2281 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     2341 atcgccttct atcgccttct tgacgagttc ttctgagggg atcaattctc tagagctcgc
     2401 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2461 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2521 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2581 aagggggagg attgggaaga gaatagcagg catgctgggg atgcggtggg ctctatggct
     2641 tctgaggcgg aaagaaccag ctgggggcta gcaaagccta ggaacagctg catatagcac
     2701 tgcagtttac tccctatcag tgatagagag tcaaaaggcc atgccctccc tatcagtgat
     2761 agagagagca gactcaaggt ggtccctatc agtgatagag agattgagca agagggcctc
     2821 cctatcagtg atagagattg atccagtcag ggcctcccta tcagtgatag agaagctatg
     2881 tgcaggtagg gctgtacagt gggaatgcac gtaggatctg ccattgctcc agtgcccttc
     2941 agtgggcaga gagcacatca cccacagtcc ccaagaagtt ggggggaggg gtgggcaatt
     3001 gaactggtgc ctagagaagg tggcgcaggg taaactggga aagtgatgtc gtgtactggc
     3061 tccgcctttt tcccgagggt gggggagaac catatataag tgcagtagtc cccgtgaacg
     3121 ttcttttttg caacgggttt gccgccagaa cacagctgaa gctttgaggg gctagcatct
     3181 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acaccggttg agttgcgttc
     3241 tgccgcctcc cgcctgtggt gcctcctgaa ctgtgtccgc cgtctaggta agtttaaagc
     3301 tcaggttgag accgggcctt tgtccggtgc tcccttggag cctacctaga ctcagccggc
     3361 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     3421 tgcgccgtta cagatccaag ctgtgacctg ggcctacACC TGGCCgctct agcccgggcg
     3481 gatcccccgg gctgcaggaa ttcgatatca agcttgccac catgccagag ccagcgaagt
     3541 ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg
     3601 gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga
     3661 agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg
     3721 tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc
     3781 gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt
     3841 tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg
     3901 atccccgggt accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     3961 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     4021 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     4081 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
     4141 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     4201 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     4261 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     4321 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     4381 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     4441 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4501 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     4561 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4621 gcatggacga gctgtacaag taggtcgacc tcgagggggg gcccggtacc aggtaagtgt
     4681 acccaattcg ccctatagtg agtcgtatta caattcactc gatcgccctt cccaacagtt
     4741 gcgcagcctg aatggcgaat ggagatccaa tttttaagtg tataatgtgt taaactactg
     4801 attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag gcccctcagt
     4861 cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt tttacttgct
     4921 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4981 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5101 tcttaacgcg taaattgtaa gcgtctcaaa tatgtatccg ctcatgagac aataaccctg
     5161 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5221 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5281 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5341 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5401 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5461 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5521 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5581 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5641 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5701 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5761 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5821 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5881 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5941 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6001 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6061 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6121 gatctaggtg aagatccttt ttgataatc
//