LOCUS       pMC-1-TBS-11            4253 bp ds-DNA     circular     23-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    90..2095
                     /label="TBS enhancer-blocking insulator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2096..2099
                     /label="J11"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2221..2325
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2326..3336
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3488..4253
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3507..4095
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaG GAGaaccttt tatgtacttc acaaccctca
      121 atgctgcttc caggtgtgac tgtttgggtt gctgcatgaa ttgactcagc aGctgcactg
      181 caaagctgat atctggcctt gtgattgtca agtagagaag ctttcccacc agtctctgat
      241 aggatcccac atcctctaac tctgcatcat cagtctttcc taagtgcttg tcatattcta
      301 cagtagtcag cctttggttc tgttccattg gggttgacac tggtctgcag cctcccagac
      361 caacacctga tatcagttcc agtgcatact tcctctggtt cagtaggatt cccttttctg
      421 atctcagcac ctcaatgcct aagaagtatt ttagttctcc caaatctttc atcttgaaat
      481 gctgatgcag ggttgccttt gcttctgaaa tcaaaacatt gctgctgcct gttattaaca
      541 gatcatccac ataaatcagg attatgacaa ggtcagtccc ctctcttttg gtaaacaagg
      601 agtgatcata agcactttgc ataaaaccag cctgcataag gacagtggta agcttgatgt
      661 tccactgcct tgatgcttgc tttaaaccat agagggattc aacagcctgc acactttggt
      721 Gtccccttgg ctgtgaaaac cctgaggcag agacatataa acttcttcca tgaggtcacc
      781 ttgtagaaaa gcattgttga catccatctg gaaaaggaac cagcccttgg aagcagcaac
      841 agatatgaca gcttttacag tgaccatttt ggccactgga gaaaaagttt catggtagtc
      901 aaggccttct tgctgagtgt atcccttggc cactagcctt gccttaaacc tgtcaacttc
      961 accattagct ttgtatttaa ttttgtacac ccatttggac cctataggct gtttaccagg
     1021 gggtaaaggg acaatctccc aggtgttatt atcctcaaga gcctgtatct caagggacat
     1081 ggcctccatc catttctcat cttgagctgc ttctttgaag gatttaggtt cagtataagt
     1141 ggagaaagca ctcaaataag cttgatagtg aacaggcaag tgatcatagg aaacatagtt
     1201 ggctataggg tatggaacat ccctagagcc tttgttcagt gtcacaaagt ccttgagcca
     1261 gatgggagga cctgcattgc gtttaggtct agtgtgcagg tttgctggaa ctagagaggg
     1321 atcagctaca gcagtatggt gctcaaattc agcatttgct aggtcaggct cagctgactc
     1381 aactgactct gcaggagcat gcaCgtggcc tgaaggtgca gcatcagctg aagtgatatg
     1441 attttgggta gaatgagtcc ccaaagtgat atcctcattg gcctctacaa tgtcaggaat
     1501 aagaactgga gtatcatcat catcagtatg aaagctggaa ggaaaaatat cattatacac
     1561 tagctgcaaa gctgtatctt cagagctaag tgaacctgct cgagtgaaca tatcaggttc
     1621 atgggagatg gactctttga aagggaactg aaactctctg aaaactacat ccctgctcac
     1681 tatgatcacc ttattatcca agtcatacaa cctgtaaccc ttttgagtct cagaataacc
     1741 aatgaagatg gttcgcttgg ctctaggtgc aagtttgtca cctttgggca gtgttgctgc
     1801 aaaagcaaga cacccaaaca ctctcaagtg atcaagcttg gcatgtttct gatagagtag
     1861 ttcatatgga catttgcctt gtaagattgg agtagggagt ctattgatca ggtatacaac
     1921 agtcttgaca cagtctcccc aaaacctggt aggtacacca ctctgaaact taagtgccct
     1981 tgccatctca aggatgtgtc tgtgctttct ctccacaaca ccattctgtt gtggtgtgta
     2041 gggacagcta ctttgatgaa caatcccaag agaggccagc aactcattac aacttCGCTt
     2101 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     2161 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     2341 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2401 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     2461 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     2521 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
     2581 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     2641 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt
     2701 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     2761 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     2821 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     2881 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     2941 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     3001 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     3061 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     3121 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     3181 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     3241 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac
     3301 gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt ttactcatat
     3361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     3661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4201 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cga
//