LOCUS       pMC-1-PvUbi1-6          4234 bp ds-DNA     circular     23-MAY-2021
DEFINITION  .
ACCESSION   urn.local...ab3nk5i-6b                                             
VERSION     urn.local...ab3nk5i-6b                                             
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        94..682
                     /label="PvUbi1 promoter"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     5'UTR           683..775
                     /label="PvUbi1 5'UTR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     intron          776..2066
                     /label="PvUbi1 intron1"
                     /ApEinfo_revcolor=#eb606c
                     /ApEinfo_fwdcolor=#eb606c
     misc_feature    2077..2080
                     /label="J6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2307..3317
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3469..4234
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3488..4076
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcacgtca gtgtttggtt tccactagca
      121 cgagtagcgc aatcagaaaa ttttcaatgc atgaagtact aaacgaagtt tatttagaaa
      181 tttttttaag aaatgagtgt aattttttgc gacgaattta atgacaataa ttaatcgatg
      241 attgcctaca gtaatgctac agtaaccaac ctctaatcat gcgtcgaatg cgtcattaga
      301 ttcgtctcgc aaaatagcac aagaattatg aaattaattt tacaaactat ttttatttaa
      361 tactaataat taactgtcaa agtttgtgct actcgcaaga gtagcgcgaa ccaaacacgg
      421 cctggaggag cacggtaacg gcgtcgacaa actaacggcc accacccgcc aacgcaaagg
      481 agacggatga gagttgactt cttgacggtt ctccacccct ctgtctctct gtcactgggc
      541 cctgggtccc cctctcgaaa gttcctctgg ccgaaattgc gcggcggaga cgaggcgggc
      601 ggaaccgtca cggcagagga ttccttcccc accctgcctg gcccggccat atataaacag
      661 ccaccgcccc tccccgttcc ccatcgcgtc tcgtctcgtg ttgttcccag aacacaacca
      721 aaatccaaat cctcctcctc ctcccgagcc tcgtcgatcc ctcacccgct tcaaggtacg
      781 gcgatcctcc tctcccttct cccctcgatc gattatgcgt gttccgtttc cgtttccgat
      841 cgagcgaatc gatggttagg acccatgggg gacccatggg gtgtcgtgtg gtggtctggt
      901 ttgatccgcg atatttctcc gttcgtagtg tagatctgat cgaatccctg gtgaaatcgt
      961 tgatcgtgct attcgtgtga gggttcttag gtttggagtt gtggaggtag ttctgatcgg
     1021 tttgtaggtg agattttccc catgattttg cttggctcgt ttgtcttggt tagattagat
     1081 ctgcccgcat tttgttcgat atttctgatg cagatatgat gaataatttc gtccttgtat
     1141 cccgcgtccg tatgtgtatt aagtttgcag gtcctagtta ggtttttcct actgatttgt
     1201 cttatccatt ctgtttagct tgcaaggttt ggtaatggtc cggcatgttt gtctctatag
     1261 attagagtag aataagatta tctcaacaag ctgttggctt atcaattttg gatctgcatg
     1321 tgtttcgcat ctatatcttt gcaattaaga tggtagatgg acatatgctc ctgttgagtt
     1381 gatgttgtac cttttacctg aggtctgagg aacatgcatc ctcctgctac tttgtgctta
     1441 tacagatcat caagattatg cagctaatat tcgatcagtt tctagtatct acatggtaaa
     1501 cttgcatgca cttgctactt atttttgata tacttggatg ataacatatg ctgctggttg
     1561 attcctacct acatgatgaa cattttacag gccattagtg tctgtctgta tgtgttgttc
     1621 ctgtttgctt cagtctattt ctgtttcatt cctagtttat tggttctctg ctagatactt
     1681 accctgctgg gcttagttat catcttatct cgaatgcatt ttcatgttta tagatgaata
     1741 tacactcaga taggtgtaga tgtatgctac tgtttctcta cgttgctgta ggttttacct
     1801 gtggcaactg catactcctg ttgcttcgct agatatgtat gtgcttatat agattaagat
     1861 atgtgtgatg gttctttagt atatctgatg atcatgtatg ctcttttaac ttcttgctac
     1921 acttggtaac atgctgtgat gctgtttgtt gattctgtag cactaccaat gatgacctta
     1981 tctctctttg tatatgatgt ttctgtttgt ttgaggcttg tgttactgct agttacttac
     2041 cctgttgcct ggctaatctt ctgcagatcc tgcaggaatg tgagaccacg aagtggctct
     2101 tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2281 ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg
     2341 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2401 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2461 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2521 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2581 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2641 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     2701 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2761 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2821 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2881 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2941 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3001 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3061 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3121 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3181 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3241 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     3301 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     3361 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4201 ccgctcgccg cagccgaacg accgagcgca gcga
//