LOCUS       pMC-1-SlEf1a-6          3482 bp ds-DNA     circular     23-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    90..550
                     /label="SlEf1a promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    551..1305
                     /label="SlEf1a intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1306..1324
                     /label="SlEf1a 5' UTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1325..1328
                     /label="J6"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    1450..1554
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1555..2565
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2717..3482
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2736..3324
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaG GaGGGTTTCT ATTTGTAAGC TAATCTTTTT
      121 CAGTTATCCT TTTTTTTAAA TCTTTATTAT TATTATAGCT ATATCTTTTA TCTTTTAAAA
      181 TTAACATTAT CTATTAAAGA TAATTTCAAT AAAAGAGTAA AAATTAATTT AGAGTTCTAC
      241 TTTCTTCAAA TTTCTATTTT AAAAAATAAT TTTAAAACTT GATGTATTTT TTTACGTGGT
      301 TTTTCACTAT GATTTGATTT CTGTTTTATT ATAATATGTA TAAATATAAA AATAGATATT
      361 CCATAACATA TTATAAAAAA TGTAAGGGGC ATTTACGTAA ATAGATAGAC TTAAAAGAGG
      421 CACCGAGTGA ACCCTAATTC TCATCGTTGA GACTATAAAA TGCCCATTAT CCCATTCGCA
      481 GTCTCTTCAT TACTTTTGCT GTgaTTtCTC CTCaGCTGTG CCGCATATCG CCTAATTTTT
      541 CTTCTCTAAG GTTTCGTCAT CTTCACCAAT TTCTTTAATC TCGATTCTAT TTTTTATGTT
      601 TGATCTGTTA TTGTTCTGTC ACTACATGTG TTTTTCAGTT GTTTTACTAG ATGATTTTCA
      661 CTGaCTTCTT GTTAGATCAT ACATATATTG AAAATGTTTT GGATTGACTT TTTTGTATTG
      721 TGAATATCTG TTATTGTTTG ATTGTTGTTC AGTATTTACA CAGCCGATCT GTGTTATGAG
      781 CTTGGTaATA ACTATTTCTC TGTATGTAAA TACAGATCTG TTAATGTTTG TAATCAATTT
      841 TTCATATGCA CTGTTGATAT TGTTCTCTCT CCTGTCCTGT TATATGTTGG TATGATTCGG
      901 TTTTTGTATA ACTTGAACTA AACACTAGTC CTAAATGTTT TTTTTACTAT TTAAGATTTA
      961 TATAATATGG ATAGATTTTT TGAGTTCCTA GTCTCTGAAG AGGTTAAGCT TGCTGTAGTT
     1021 GTTTACCAGT TGAGTTGCAA TACTAAAAAT CAATTCAATT ACTGATATTT TTTGCCGTTT
     1081 AGGTTTTTGA CAAAGTACTT TAATTTGCTT TATTGAACTA AAAACGTAGT CCTGAATTCA
     1141 TGGCAAGTGT GAAAGCTATA GTTCACTGTT TTTGTTGCAA TTCTTGAAAA ATCAATTGGT
     1201 CAAGCTATAA TGGATTTACT TTTTCTGTTT TAAATGTTGA ATGCGCTGAA TTTATGAATG
     1261 GGTTGCATGG TTTTTGAAAT ATGTTGTTGT GTGTTGTGTA AATGCAGTTT CTTAGTGTCT
     1321 CAAGaatgtg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
     1381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc
     1561 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1621 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     1681 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     1741 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     1801 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     1861 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat
     1921 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     1981 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     2041 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     2101 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     2161 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     2221 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     2281 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     2341 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     2401 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     2461 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     2521 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
     2581 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2641 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2701 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2761 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2821 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2881 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2941 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3001 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3061 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3421 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3481 ga
//