LOCUS       pMC-1-SlRPS5A1-6        3578 bp ds-DNA     circular     30-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    94..1421
                     /label="SlRPS5A1 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1421..1424
                     /label="J6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1546..1650
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1651..2661
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2813..3578
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2832..3420
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaG GAGCCAACAC CAAGTGATAT ACTAACCATG
      121 TAAATAATCC AAATTTATCC AAAAATATCA TTATCTAAAG TGTGTAAACA AAAATCAAAT
      181 ATGAAATACT TACATACCTT TCCGTATCTG ACCGTTTAAC TCTAAAATTG AAGCTAGTTT
      241 TAATTTTGTA TTTCATCATT ACAGCCACAA GAGTTGCTTT ATCCTTGTAC AATTGTTTCG
      301 TCTACACATT TAATATATTT GTATCTGATA TATAATTTTC TTTCTCCATC TCGGGTATGT
      361 AAAAAGAATC ACCAATGTTT GATTCAACAA CGTTTAGAGC ATTAGCATCT CTTTGTCCAC
      421 CTTCAATACA CATAATTGCA CCACTGCTTG AATAAAAAAT TTGTAACTCC TCTGTACTTT
      481 TATCATTTCT ATCGATGCAA AGCGGATACA TACTGAATCC AACCTCTTGC TTCTTTATTT
      541 CTATAAACAG CTTCACACCA ATATCATTCC TAATTATCAT CGGAGAAGAA TTGCCCTCTA
      601 CAATATATCG GATAATAATA TTTTTTTTCA ATTCATCAAT ATTTAATTCA GTAGCAATTG
      661 CTGAGATTAG ATTCATATAT GAAATATTTT CCCCAACCAC TATCCCATCA CTTTTGTATT
      721 GATTGTATTT AACCTCACTC TCCCATATTC CTGAATGCCT TAGCAATATC GTAATATTCA
      781 TTACGAAACC GGATACAAAA TTACCTTCAA GGAAATTTTG AATATCGTTT GTGCCAAAAA
      841 AATGGAAGAT CACTAATCAA AATCAAAATA TCATTTTTGC CAAAAATAAA AAGAAAGATC
      901 ACTAATGACA AAATTTAAGG GAATAAAACT GATTTTAAAA CACACAAGTT AATGCCTTTA
      961 TTTAAGGGAA TAATAACCGT GGCATATTAA AAGTAGGAAT TAATTCAAAT TTCAGTTTTT
     1021 AATCCCGCAA ATTACTCAAC ATACAAATAA AAATCCCCCA AATTACTCAA CAAACGGATA
     1081 AAATGAAATA CTATATAATG TATCCGACAA TTTTATTATG TATCATAAAC TTATTAAAAA
     1141 TAAAAGATTT TTTGTAAATT GAAAAAGAAT AGGGATTGGA GGTAATTTAT ATTTTACACT
     1201 ATGTGATTTA CATAATTTTT ACATTTTTTA TTGAAAAACT TTAGAAGTTG CGCAAGTAGC
     1261 CAAAAAAACA AACTGGAGCA AGTTGAGTTA GGAAAAGTGG GCCAAATAGG CTTTTTAGCC
     1321 CAATCTTAGA ATGAAGGCTA TTTTGGACAG TATAAGTGGG TTTAGGGTTT TCCTTCTTCT
     1381 CCAATCGACA CCTCCTTAGA CCTATCAGAA AAACAGGAAA AATGtgagac cacgaagtgg
     1441 ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1501 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1621 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
     1681 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     1741 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     1801 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     1861 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     1921 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     1981 gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg
     2041 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     2101 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     2161 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     2221 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     2281 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     2341 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     2401 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     2461 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     2521 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     2581 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc
     2641 accaaggtag tcggcaaata actgtcagac caagtttact catatatact ttagattgat
     2701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3541 gataccgctc gccgcagccg aacgaccgag cgcagcga
//