LOCUS       pMC-1-SlUbi-6           3489 bp ds-DNA     circular     23-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    92..738
                     /label="SlUbi promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    739..837
                     /label="5' UTR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    838..1331
                     /label="Intron"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1332..1335
                     /label="J6"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    1457..1561
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1562..2572
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2724..3489
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2743..3331
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gAGACATAAA TTCTTTTTTT GTAATGCTCA
      121 AATAAATTTG AGTAAAAAAG AATGAAATTG AGTGATTTTT TTTTAATCAT AAGAAAATAA
      181 ATAATTAATT TCAATATAAT AAAACAGTAA TATAATTTCA TAAATGGAAT TCAATACTTA
      241 CCTCTTAGAT ATAAAAAATA AATATAAAAA TAAAGTGTTT CTAATAAACC CGCAATTTAA
      301 ATAAAATATT TAATATTTTC AATCAAATTT AAATAATTAT ATTAAAATAT CGTAGAAAAA
      361 GAGCAATATA TAATACAAGA AAGAAGATTT AAGTACAATT ATCAACTATT ATTATACTCT
      421 AATTTTGTTA TATTTAATTT CTTACGGTTA AGGTCATGTT CACGATAAAC TCAAAATACG
      481 CTGTATGAGG ACATATTTTA AATTTTAACC AATAATAAAA CTAAGTTATT TTTAGTATAT
      541 TTTTTTGTTT AACGTGACTT AATTTTTCTT TTCTAGAGGA GCGTGTAAGT GTCAACCTCA
      601 TTCTCCTAAT TTTCCCAACC ACATAAAAAA AAAATAAAGG TAGCTTTTGC GTGTTGATTT
      661 GGTACACTAC ACGTCATTAT TACACGTGTT TTCGTATGAT TGGTTAATCC ATGAGGCGGT
      721 TTCCTCTAGA GTCGGCCATA CCATCTATAA AATAAAGCTT TCTGCAGCTC ATTTTTTCAT
      781 CTTCTATCTG ATTTCTATTA TAATTTCTCT GAATTGCCTT CAAATTTCTC TTTCAAGGTT
      841 AGAATTTTTC TCTATTTTTT GGTTTTTGTT TGTTTAGATT CTGAGTTTAG TTAATCAGGT
      901 GCTGTTAAAG CCCTAAATTT TGAGTTTTTT TCGGTTGTTT TGATGGAAAA TACCTAACAA
      961 TTGAGTTTTT TCATGTTGTT TTGTCGGAGA ATGCCTACAA TTGGAGTTCC TTTCGTTGTT
     1021 TTGATGAGAA AGCCCCTAAT TTGAGTGTTT TTCCGTCGAT TTGATTTTAA AGGTTTATAT
     1081 TCGAGTTTTT TTCGTCGGTT TAATGAGAAG GCCTAAAATA GGAGTTTTTC TGGTTGATTT
     1141 GACTAAAAAA GCCATGGAAT TTTGTGTTTT TGATGTCGCT TTGGTTCTCA AGGCCTAAGA
     1201 TCTGAGTTTC TCCGGTTGTT TTGATGAAAA AGCCCTAAAA TTGGAGTTTT TATCTTGTGT
     1261 TTTAGGTTGT TTTAATCCTT ATAATTTGAG TTTTTTCGTT GTTCTGATTG TTGTTTTTAT
     1321 GAATTTTGCA Gaatgtgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     1381 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1441 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1501 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1561 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1621 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     1681 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1741 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1801 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     1861 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     1921 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     1981 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     2041 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     2101 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     2161 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     2221 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     2281 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     2341 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     2401 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     2461 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     2521 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     2581 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2641 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2881 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3301 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3361 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3421 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3481 gcgcagcga
//