LOCUS       pMC-1-StUbi-6           3869 bp ds-DNA     circular     23-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    91..1043
                     /label="StUbi promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1044..1142
                     /label="5' UTR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1143..1711
                     /label="Intron"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1712..1715
                     /label="J6"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    1837..1941
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1942..2952
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3104..3869
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3123..3711
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaG GAGGATGACA AGATTTTGGT CATCAATTAC
      121 ATATACCCAA ATTGAATAGT AAGCAACTTA ATGTTTTTCA TAATGATAAT GACAGACACA
      181 AAAAAAACCC ATTTATTATT CACATTGATT GATTTTTATA TGCAATATAG TAATAATAAT
      241 AATATTTCTT ATAAAGCAAG AGGTCAATTT TTTTTTATTA TACCAACGTC ACTAAATTAT
      301 ATTTGATAAT GTAAAACAAT TCAATTTTAC TTAAATATCA TGAAATAAAC TATTTTTATA
      361 ACCAAATTAC TAAATTTTTC CAATAAAAAA AAGTCATTAA GAAcACATAA AATAAATTTG
      421 AGTAAAAAGA GTGAAGTCGA CTGACTTTTT TTTTTTTTAT CATAAGAAAA TAAATTATTA
      481 ACTTTAACCT AATAAAACAC TAATATAATT TCATGGAATC TAATACTTAC CTCTTAGAAA
      541 TAAGAAAAAG TGTTTCTAAT AGACCCTCAA TTTACATTAA ATATTTTCAA TCAAATTTAA
      601 ATAACAAATA TCAATATGAG GTCAATAACA ATATCAAAAT AATATGAAAA AAGAGCAATA
      661 CATAATATAA GAAAGAAGAT TTAAGTGCGA TTATCAAGGT AGTATTATAT CCTAATTTGC
      721 TAATATTTAA ACTCTTATAT TTAAGGTCAT GTTCATGATA AACTTGAAAT GCGCTATATT
      781 AGAGCATATA TTAAAATAAA AAAATACCTA AAATAAAATT AAGTTATTTT TAGTATATAT
      841 TTTTTTACAT GACCTACATT TTTCTGGGTT TTTCTAAAGG AGCGTGTAAG TGTCGACCTC
      901 ATTCTCCTAA TTTTCCCCAC CACATAAAAA TTAAAAAGGA AAGGTAGCTT TTGCGTGTTG
      961 TTTTGGTACA CTACACCTCA TTATTACACG TGTCCTCATA TAATTGGTTA ACCCTATGAG
     1021 GCGGTTTCGT CTAGAGTCGG CCATGCCATC TATAAAATGA AGCTTTCTGC ACCTCATTTT
     1081 TTTCATCTTC TATCTGATTT CTATTATAAT TTCTCTCAAT TGCCTTCAAA TTTCTCTTTA
     1141 AGGTTAGAAA TCTTCTCTAT TTTTGGTTTT TGTCTGTTTA GATTCTCGAA TTAGCTAATC
     1201 AGGTGCTGTT ATAGCCCTTA ATTTTGAGTT TTTTTTCGGT TGTTTTGATG GAAAAGGCCT
     1261 AAAATTTGAG TTTTTTTACG TTGGTTTGAT GGAAAAGGCC TACAATTGGA GTTTTCCCCG
     1321 TTGTTTTGAT GAAAAAGCCC CTAGTTTGAG ATTTTTTTTC TGTCGATTCG ATTCTAAAGG
     1381 TTTAAAATTA GAGTTTTTAC ATTTGTTTGA TGAAAAAGGC CTTAAATTTG AGTTTTTCCG
     1441 GTTGATTTGA TGAAAAAGCC CTAGAATTTG TGTTTTTTCG TCGGTTTGAT TCTGAAGGCC
     1501 TAAAATTTGA GTTTCTCCGG CTGTTTTGAT GAAAAAGCCC TAAATTTGAG TTTCTCCGGC
     1561 TGTTTTGATG AAAAAGCCCT AAATTTGAGT TTTTTCCCCG TGTTTTAGAT TGTTTGGTTT
     1621 TAATTCTCGA ATCAGCTAAT CAGGGAGTGT GAAAAGCCCT AAAATTTGAG TTTTTTTCGT
     1681 TGTTCTGATT GTTGTTTTTA TGAATTTGCA Gaatgtgaga ccacgaagtg gctcttcagt
     1741 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1921 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     1981 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     2041 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     2101 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     2161 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     2221 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     2281 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     2341 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     2401 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     2461 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     2521 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     2581 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     2641 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     2701 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     2761 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     2821 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     2881 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     2941 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3241 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3841 cgccgcagcc gaacgaccga gcgcagcga
//