LOCUS       pMC-1-BeYDV-LS-L-11     3020 bp ds-DNA     circular     01-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    94..387
                     /label="LIR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    207..248
                     /label="stem loop structure"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(220..228)
                     /label="nononucleotide (v-sense ORI)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    388..539
                     /label="SIR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    540..543
                     /label="M1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    540..569
                     /label="Esp3I entry point"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    566..569
                     /label="M omega"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    570..863
                     /label="LIR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    683..724
                     /label="stem loop structure"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(696..704)
                     /label="nononucleotide (v-sense ORI)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    863..866
                     /label="J11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    988..1092
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1093..2103
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2255..3020
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2274..2862
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcaaatac catcacatcg tatatgcttt
      121 tatagtgtga acacctttaa ccctagtggg cgggaaattt tctactttaa atctggaccg
      181 ctcgtgctaa agcactcgcg ataagggggg gccacgccgg taatattaaa ttcggcgtgg
      241 gccccccctt gtcgcaaaga cttcgtcttt aagtaaattc cacgtcattt tccactatct
      301 attaaaatga ccaaaatacc cctgcctcca tgcctccacg ccggttataa gatagagttt
      361 gaggcaaccc ctcggagtca caacaacacg tgcctctcct catacgagtt tatctaaagt
      421 gattattttt tttggggtgt ttgtttttgg attgtctttt tttgtttatt tcgtgtgtta
      481 tgtaacatat gtaatttcta tctacttgca caatgaaata tattcataaa ataatcatta
      541 ccGtGAGACG aacaacaaCG TCTCtAGTGc aaataccatc acatcgtata tgcttttata
      601 gtgtgaacac ctttaaccct agtgggcggg aaattttcta ctttaaatct ggaccgctcg
      661 tgctaaagca ctcgcgataa gggggggcca cgccggtaat attaaattcg gcgtgggccc
      721 ccccttgtcg caaagacttc gtctttaagt aaattccacg tcattttcca ctatctatta
      781 aaatgaccaa aatacccctg cctccatgcc tccacgccgg ttataagata gagtttgagg
      841 caacccctcg gagtcacaac aacgcttgag accacgaagt ggctcttcag tggacgaaag
      901 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      961 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1081 aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     1141 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     1201 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1261 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     1321 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1381 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1441 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1501 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1561 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1621 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1681 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1741 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1801 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1861 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1921 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1981 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     2041 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     2101 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2161 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2221 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2281 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2341 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2401 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2461 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2521 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2581 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2641 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2701 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2761 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2821 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2881 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2941 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3001 cgaacgaccg agcgcagcga
//