LOCUS       pMC-1-WDV-LRS-L-11      4410 bp ds-DNA     circular     01-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    94..502
                     /label="LIR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(119..122)
                     /label="TATA box for virion sense expression"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    245..290
                     /label="stem loop structure"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(262..270)
                     /label="nononucleotide (v-sense ORI)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    375..380
                     /label="TATA box for complementary sense expression"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    503..505
                     /label="J6"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    503..1132
                     /label="Rep"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    503..1297
                     /label="RepA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1082..1096
                     /label="Rb-binding domain (LxCxE)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1133..1218
                     /label="Intron"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1188..1192
                     /label="ctgac lariat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1219..1644
                     /label="Rep"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_structure  1255..1278
                     /label="NTP binding motif"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1645..1815
                     /label="SIR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1815..1818
                     /label="M1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1815..1844
                     /label="Esp3I entry point"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1841..1844
                     /label="M omega"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1845..2253
                     /label="LIR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1870..1873)
                     /label="TATA box for virion sense expression"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1996..2041
                     /label="stem loop structure"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2013..2021)
                     /label="nononucleotide (v-sense ORI)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2126..2131
                     /label="TATA box for complementary sense expression"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2253..2256
                     /label="J11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2378..2482
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2483..3493
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3645..4410
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3664..4252
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcgagatg ggctaccacg cacttcctta
      121 taagctaagg catggcacag atttccccgt caaaagtgtg ataaatcaac atcttccacg
      181 aaagtgatca gaaaagatgt ggtccaaaag cctccgcgca ctcacgaaaa gccgagtgcg
      241 cgtcgggggc caccacgcgg ggtaatatta taacccgcgt ggaggccccc cgaccgcaca
      301 ccgaacaggg cccacacgat tcgctacgct accgtgggaa cactagggcc ctgttctccc
      361 gccaaaacct gccctatata aagcattgga cacattgcat ttgcagtgtg cagaattcac
      421 acctccacgc agggtagagg atagagtttt ctggcacacc ccgttttttc gctaaggacc
      481 tgccggactt ctgttcacta ccatggcctc ttcatctgca cccaggttcc gtgtctattc
      541 caagtacctc tttctaacat atcctcaatg tacccttgag ccacagtacg ccttggattc
      601 acttcgcact ctcttgaaca aatatgagcc cctctacatc gctgctgtta gagagctGca
      661 cgaagatgga tcaccacatc tgcacgttct cgtgcagaac aagcttcgtg cttccatcac
      721 caatcccaat gccttaaacc tccgtatgga tacatctcca ttctccatat tccatccaaa
      781 tatacaagct gccaaagact gcaaccaagt tcgtgattac atcacgaagg aggttgactc
      841 cgatgtaaac acagctgagt ggggaacatt cgtggctgtt tcaactccag gtcgtaaaga
      901 ccgtgatgcg gatatgaaac agatcattga atctagttcc tctcgcgagg aattcctcag
      961 catggtttgc aatcgttttc cgtttgaatg gtctatccgC ctcaaagact tcgagtacac
     1021 ggcacgccat ctatttcctg acccagttgc cacttacaca cctgagtttc caaccgaatc
     1081 actcatttgc catgagacAa ttgaaagctg gaaaaatgaa catctctact ccgtaagcct
     1141 cgaatcctat atcctttgta cttccactcc tgcggatcaa gcgcaatctg acttagagtg
     1201 gatggacgat tattccagga gtcaccgggg aggcataagt ccatctacat ctgcgggcca
     1261 accagaacag gaaagacttc ctgggcaagg tctTtaggga cacacaatta ttataacagt
     1321 ctagttgatt tcacaacata tgacgtcaac gccaagtata atatcatcga cgacattcca
     1381 ttcaagttca cacccaactg gaagtgcttc gtcggggctc agcgtgactt cacggtcaat
     1441 ccaaaatatg gtaagcgaaa ggtcatacgg ggtggaatac cttgcatcat tttagttaat
     1501 ccagacgaag attggctcaa ggatatgact cccgaacagt cggattacat gtactctaac
     1561 actgttgttc actacatgta tgaaggcgag acAttcatca actactcgtt cgcctccggc
     1621 gaagaTgtca ctgcttcgca gtgactgtcc tagcgcggac ggcgtacagt ttctataaat
     1681 gtaatatctc agggtgttgt atttcgtttt ccgatgtcat tgtgtaaggg ttgttgtttg
     1741 ttatatttgg tttgtctttg ctcgtagccg agcctctgta atcgaatgac atgagataaa
     1801 taaaatatta ttttaccGtG AGACGaacaa caaCGTCTCt AGTGcgagat gggctaccac
     1861 gcacttcctt ataagctaag gcatggcaca gatttccccg tcaaaagtgt gataaatcaa
     1921 catcttccac gaaagtgatc agaaaagatg tggtccaaaa gcctccgcgc actcacgaaa
     1981 agccgagtgc gcgtcggggg ccaccacgcg gggtaatatt ataacccgcg tggaggcccc
     2041 ccgaccgcac accgaacagg gcccacacga ttcgctacgc taccgtggga acactagggc
     2101 cctgttctcc cgccaaaacc tgccctatat aaagcattgg acacattgca tttgcagtgt
     2161 gcagaattca cacctccacg cagggtagag gatagagttt tctggcacac cccgtttttt
     2221 cgctaaggac ctgccggact tctgttcact accgcttgag accacgaagt ggctcttcag
     2281 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2341 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2401 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2461 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2521 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     2581 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2641 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2701 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2761 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2821 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2881 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2941 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     3001 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     3061 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3121 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3181 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3241 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3301 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3361 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3421 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     3481 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3781 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4381 tcgccgcagc cgaacgaccg agcgcagcga
//