LOCUS       pMC-6-FLPe-9            3546 bp ds-DNA     circular     17-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    91..117
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    91..1389
                     /label="FLPe recombinase (tomato codon optimized)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1389..1392
                     /label="J9"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1514..1618
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1619..2629
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2781..3546
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2800..3388
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGGCTCCAA AAAAGAAACG AAAAGTAATG
      121 AGCCAGTTTG ACATCCTCTG TAAGACTCCT CCTAAGGTGC TTGTGCGTCA ATTTGTCGAA
      181 CGTTTCGAGC GTCCTTCTGG TGAAAAGATA GCTAGTTGCG CCGCCGAGCT TACCTACTTA
      241 TGCTGGATGA TTACTCATAA CGGTACTGCA ATAAAGAGGG CTACCTTTAT GAGTTACAAT
      301 ACTATAATAT CAAACAGCTT GAGTTTTGAC ATCGTAAATA AGAGTTTACA ATTCAAATAC
      361 AAAACTCAAA AAGCTACAAT ACTCGAGGCA AGTCTCAAAA AGCTTATTCC AGCTTGGGAA
      421 TTTACAATAA TCCCCTATAA CGGCCAGAAG CATCAATCTG ACATTACTGA CATCGTTAGT
      481 AGCCTCCAAC TGCAATTTGA GAGTAGTGAA GAGGCTGACA AAGGGAACTC ACACTCAAAG
      541 AAGATGCTGA AAGCTTTATT ATCTGAGGGC GAGAGCATTT GGGAAATCAC CGAGAAAATA
      601 CTCAATAGTT TTGAATATAC ATCTAGATTT ACCAAGACAA AAACACTGTA CCAATTCCTA
      661 TTCTTAGCCA CATTTATAAA TTGCGGGAGA TTCTCAGATA TCAAAAACGT GGATCCCAAA
      721 AGCTTTAAGT TAGTTCAGAA CAAGTATCTC GGGGTAATTA TCCAATGTTT AGTTACTGAA
      781 ACCAAGACAA GCGTGAGTCG TCACATCTAC TTTTTTTCAG CACGAGGGAG GATCGATCCA
      841 TTGGTTTACC TTGACGAATT TCTAAGAAAT TCAGAACCAG TATTGAAACG TGTCAATCGT
      901 ACAGGGAATT CATCTTCAAA TAAGCAGGAG TACCAACTAT TAAAAGACAA CCTCGTGAGA
      961 AGTTACAACA AAGCACTTAA AAAGAATGCA CCCTATCCTA TATTTGCAAT AAAGAATGGT
     1021 CCCAAATCTC ACATCGGTCG TCACTTGATG ACATCTTTCC TGTCTATGAA GGGCCTCACC
     1081 GAACTCACAA ATGTAGTTGG GAACTGGTCC GATAAAAGGG CTTCAGCTGT AGCTCGAACT
     1141 ACATATACAC ATCAGATAAC CGCTATACCA GACCACTATT TTGCTCTTGT TTCCAGATAT
     1201 TACGCCTACG ACCCTATCTC CAAGGAGATG ATAGCCTTGA AAGATGAAAC TAATCCAATA
     1261 GAAGAATGGC AACACATCGA ACAACTAAAA GGAAGCGCTG AAGGGAGTAT TCGATACCCT
     1321 GCTTGGAACG GCATAATTAG TCAGGAGGTT CTTGACTATT TGTCATCTTA CATCAACCGT
     1381 AGGATTTAGC TTtgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
     1441 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1621 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1681 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1741 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1801 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1861 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1921 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
     1981 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2041 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2101 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2161 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2221 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2281 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2341 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2401 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2461 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2521 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2581 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     2641 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2701 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2941 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3121 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3181 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3241 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3301 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3361 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3421 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3481 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3541 cagcga
//