LOCUS       pMC-6-R-9               3741 bp ds-DNA     circular     17-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    91..1584
                     /label="R recombinase (tomato codon optimized)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1561..1581
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1584..1587
                     /label="J9"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1814..2824
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2976..3741
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2995..3583
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGGCTCTCA CCAAAGACAC AGAAATTAGT
      121 ACAATAAACC GTCAAATGTC AGACTTCAGC GAGCTATCAC AGATCTTACC ACTCCACCAA
      181 ATAAGCAAAA TCAAAGATAT ACTGGAGAAC GAAAATCCAC TTCCAAAAGA GAAGCTTGCC
      241 AGCCACCTCA CTATGATTAT ACTAATGGCA AACCTTGCAT CCCAGAAGCG AAAAGATGTT
      301 CCAGTGAAAA GGAGCACATT TTTGAAATAT CAGCGAAGTA TCAGTAAAAC ACTGCAGTAT
      361 GATAGCTCCA CCAAAACTGT CTCCTTCGAG TATCACTTGA AAGATCCATC CAAATTGATT
      421 AAGGGCCTAG AAGATGTAGT AAGCCCTTAT AGGTTCGTGG TTGGGGTACA CGAAAAACCC
      481 GACGATGTAA TGTCCCATCT CAGTGCTGTC CACATGCGAA AGGAAGCAGG ACGTAAGAGG
      541 GATCTGGGGA ATAAGATCAA TGATGAAATA ACTAAAATAG CAGAGACACA AGAGACTATT
      601 TGGGGATTCG TGGGCAAAAC TATGGACCTT ATCGAGGCAC GAACAACCCG ACCAACAACT
      661 AAGGCAGCTT ATAACCTTTT GTTACAAGCC ACATTCATGA ACTGTTGTAG AGCTGATGAC
      721 CTCAAAAACA CAGATATTAA GACTTTTGAG GTAATCCCCG ATAAACACCT GGGAAGGATG
      781 CTACGTGCAT TCGTGCCCGA AACCAAAACC GGTACCAGGT TCGTATATTT CTTCCCTTGC
      841 AAAGGAAGGT GTGATCCTCT CTTGGCCCTG GATAGCTACT TACAGTGGAC CGACCCCATC
      901 CCCAAAACCA GAACTACCGA TGAAGATGCA CGATATGATT ACCAGCTCTT GAGAAATAGT
      961 TTGCTTGGCA GTTACGATGG CTTTATATCC AAGCAGAGCG ACGAGTCTAT TTTTAAGATC
     1021 CCCAACGGGC CTAAAGCTCA CCTTGGCAGG CACGTAACAG CATCATACTT GAGTAACAAC
     1081 GAGATGGATA AGGAGGCCAC TTTATACGGG AATTGGTCAG CCGCACGTGA AGAGGGTGTG
     1141 AGTCGAGTTG CCAAAGCTCG TTATATGCAT ACCATCGAGA AATCCCCCCC AAGCTATTTG
     1201 TTCGCATTTT TGAGCGGGTT TTACAATATC ACTGCTGAAA GGGCTTGCGA GTTAGTAGAC
     1261 CCAAACAGCA ACCCCTGCGA ACAAGACAAG AATATCCCCA TGATTTCTGA CATAGAGACA
     1321 CTAATGGCAC GATACGGTAA AAACGCCGAG ATTATTCCTA TGGACGTTCT CGTATTCCTG
     1381 AGTTCATACG CAAGGTTCAA GAATAACGAA GGTAAGGAGT ATAAACTCCA AGCTCGTTCA
     1441 AGTCGTGGAG TTCCAGATTT TCCAGATAAT GGTAGAACTG CCCTGTATAA TGCCTTAACT
     1501 GCCGCACACG TAAAAAGAAG GAAAATATCA ATCGTGGTCG GTAGGTCTAT TGACACTTCA
     1561 CCAAAGAAGA AGCGAAAAGT ATAGCTTtga gaccacgaag tggctcttca gtggacgaaa
     1621 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1681 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1801 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1861 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     1921 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1981 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     2041 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     2101 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     2161 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     2221 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     2281 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     2341 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     2401 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     2461 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     2521 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     2581 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     2641 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     2701 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     2761 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
     2821 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2881 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3121 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3661 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3721 ccgaacgacc gagcgcagcg a
//