LOCUS       pMC-6-Integrase4-9      3708 bp ds-DNA     circular     17-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    91..117
                     /label="SV40"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    91..1551
                     /label="Integrase4 (tomato codon optimized)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1551..1554
                     /label="J9"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1781..2791
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2943..3708
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2962..3550
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGGCCCCTA AGAAGAAGAG GAAAGTTATG
      121 ATCACAACCC GTAAGGTAGC CATCTACGTT CGTGTGTCAA CCACTAATCA GGCCGAGGAG
      181 GGGTACAGCA TACAAGGGCA AATAGATTCC CTGATAAAAT ATTGTGAAGC AATGGGCTGG
      241 ATTATCTATG AAGAATACAC AGACGCAGGG TTCAGTGGTG GAAAAATTGA CAGACCTGCT
      301 ATGAGCAAAC TGATTACCGA CGCCAAGCAC AAGAGATTTG ATACAATTCT CGTGTACAAA
      361 TTGGACAGGC TATCCAGGTC AGTGAGAGAT ACACTCTATC TCGTCAAAGA TGTTTTTAAC
      421 CAGAATAATA TCCACTTCGT GTCTTTACAG GAAAATATCG ACACCTCTAG TGCAATGGGG
      481 AATCTCTTCT TAACACTTCT ATCAGCAATT GCCGAATTTG AGCGTGAGCA GATAACCGAG
      541 AGAATGACTA TGGGGAAAAT TGGTCGTGCC AAGAGCGGCA AAACAATGGC CTGGACTTAC
      601 ACTCCCTTTG GATATGACTA CAACAAAGAA AAAGGGGAAT TAATACTGGA CCCAGCCAAA
      661 GCTCCTATAG TCAAAATGAT ATACACTGAT TACCTGAAGG GAATGTCTAT ACAGAAAATC
      721 GTGGATAAAT TAAATAAAAT GGATTACAAC GGCAAAGACT GCACCTGGTT TCCACACGGA
      781 GTGAAACATC TTTTGGATAA CCCAGTCTAC TACGGGATGA CTCGATACAA TAATAAATTG
      841 TTTCCAGGAA ACCACCAGCC TATAATTACA AAGGAATTGT TCGATAAAAC CCAAAGAGAG
      901 AGGCAAAGGA GAAGATTGGG GATAGAAGAA AACCACTATA CAATACCCTT TCAGGCAAAA
      961 TATATGCTTA GTAAGTTCTT GCGTTGCCGT CAGTGTGGTT CCCGAATGGG ACTGGAATTG
     1021 GGAAGGCCCA GGAAGAAGGA AGGGAAAAGG TCTAAAAAGT ACTATTGTCT GAACTCAAGA
     1081 CCCAAACGAA CAGCTAGTTG CGACACCCCA CTATATGACG CTGAAACCTT GGAGGACTAC
     1141 GTTCTCCATG AAATCGCAAA GATACAAAAG GATCCTAGTA TTGCAAGCAG GCAAAAGCAT
     1201 ATCGAGGACC ACGAGCTGAA GTATAAACGT GAGAGAATTG AAGCCAATAT AAACAAGACC
     1261 GTCAACCAAC TTTCTAAGCT CAACAACTTA TACCTTAACG ATCTCATCAC ACTGGAGGAC
     1321 CTAAAAACAC AGACCAATAC TCTTATTGCT AAAAAGAGGC TTTTAGAGAA TGAGTTAGAT
     1381 AAAACTTGTG ACAATGACGA TGAGCTGGAT CGTCAGGAAA CAATTGCAGA TTTCTTAGCC
     1441 CTCCCAGATG TATGGACCAT GGACTACGAA GGACAGAAGT ATGCTGTAGA GCTGCTCGTC
     1501 CAAAGAGTTA AGGTAGATCG TGACAATATA GATATCCACT GGACCTTCTA GCTTtgagac
     1561 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1621 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1801 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1861 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1921 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     1981 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2041 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2101 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     2161 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2221 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2281 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2341 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2401 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2461 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2521 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2581 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2641 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     2701 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa
     2761 aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact catatatact
     2821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcga
//