LOCUS       pMC-6-Integrase13-9     3699 bp ds-DNA     circular     02-DEC-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    91..117
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    91..1542
                     /label="Integrase13 (tomato codon optimized)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1156..1156
                     /label="SNP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1542..1545
                     /label="J9"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1772..2782
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2934..3699
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2953..3541
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGGCCCCAA AGAAAAAGAG GAAAGTTATG
      121 GCCGTCGGAA TATACATACG TGTTTCCACT CAAGAACAGG CAAGTGAAGG ACATAGCATT
      181 GAGAGTCAAA AGAAAAAACT GGCTTCCTAC TGCGAAATCC AAGGATGGGA TGACTACCGT
      241 TTCTATATTG AAGAGGGGAT TAGCGGTAAG AATACAAATA GACCAAAACT AAAACTACTA
      301 ATGGAACATA TAGAAAAGGG CAAAATCAAC ATTTTATTGG TTTACCGACT TGACCGATTA
      361 ACCAGAAGCG TTATCGATCT TCATAAGCTC CTGAACTTCC TTCAGGAACA CGGGTGTGCT
      421 TTCAAGAGTG CAACAGAAAC TTATGACACC ACAACTGCAA ATGGGCGTAT GTCAATGGGG
      481 ATCGTAAGCC TACTAGCTCA GTGGGAAACT GAAAACATGT CCGAGAGAAT CAAACTAAAT
      541 TTAGAGCACA AGGTTCTGGT GGAGGGTGAA CGAGTGGGAG CAATACCTTA TGGCTTCGAT
      601 CTCAGCGACG ATGAGAAGCT CGTCAAGAAC GAAAAATCCG CCATTCTCCT AGATATGGTC
      661 GAGCGAGTCG AAAACGGCTG GTCTGTTAAT CGAATAGTGA ACTACTTAAA CCTCACTAAT
      721 AATGACAGAA ACTGGAGTCC CAATGGAGTT CTCCGATTGC TTAGAAACCC TGCCCTATAC
      781 GGGGCAACCA GATGGAATGA TAAAATTGCC GAGAATACCC ATGAGGGAAT AATTTCTAAA
      841 GAACGATTCA ACCGACTTCA GCAAATCTTG GCTGATCGAT CAATCCACCA CCGAAGAGAT
      901 GTGAAGGGTA CTTACATCTT CCAGGGGGTC CTGAGATGCC CCGTATGCGA CCAGACACTC
      961 AGCGTCAACC GATTCATCAA AAAGCGTAAG GATGGCACCG AGTACTGTGG GGTATTATAT
     1021 AGATGCCAGC CATGTATTAA ACAAAACAAG TACAATTTAG CAATCGGGGA AGCACGTTTT
     1081 CTGAAAGCTC TAAACGAGTA TATGTCTACA GTCGAATTTC AAACAGTGGA AGATGAGGTA
     1141 ATTCCTAAGA AGAGTGAACG TGAAATGCTT GAATCACAGT TACAACAGAT CGCTCGAAAA
     1201 CGAGAAAAAT ATCAAAAGGC ATGGGCCTCT GACCTTATGA GCGACGACGA ATTTGAGAAA
     1261 TTAATGGTTG AAACTCGTGA AACTTACGAC GAGTGTAAAC AAAAGCTTGA ATCCTGCGAG
     1321 GACCCTATCA AGATCGATGA AACCTACCTA AAAGAAATTG TATACATGTT TCACCAAACA
     1381 TTTAATGACC TGGAAAGTGA GAAGCAAAAG GAATTCATAT CCAAGTTTAT TAGAACAATT
     1441 AGATATACAG TCAAGGAGCA GCAGCCTATC CGACCTGACA AATCCAAAAC AGGCAAGGGC
     1501 AAACAGAAGG TCATCATCAC TGAAGTCGAG TTTTATCAAT AGCTTtgaga ccacgaagtg
     1561 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1621 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1741 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     1801 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1861 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     1921 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     1981 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     2041 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2101 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     2161 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2221 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2281 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2341 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2401 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2461 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2521 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2581 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2641 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2701 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat
     2761 caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga
     2821 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2881 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2941 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3001 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3061 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3121 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3181 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3241 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3301 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3361 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3421 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3481 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3541 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3601 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3661 tgataccgct cgccgcagcc gaacgaccga gcgcagcga
//