LOCUS       pMC-6-ELuc-9            3876 bp ds-DNA     circular     23-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    91..1719
                     /label="E-Luc CDS (tomato codon optimized)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1719..1722
                     /label="J9"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1844..1948
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1949..2959
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3111..3876
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3130..3718
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGGAACGAG AGAAGAATGT AGTATACGGG
      121 CCAGAGCCCA AACATCCTCT CGGTAACTTT ACCGCTGGTG AAATGCTATA TAACGCCCTG
      181 CATAAGCACT CCCACATCCC CCAAGCTATC CTAGACGTTA TGGGCAACGA GAGTCTTTCT
      241 TATCAGGAAT TTTTCGACAC TACAGTAAAA CTGGGGCAGA GTTTGCAGAA TTGTGGCTAC
      301 AAAATGAATG ATGTAGTTTC TATCTGTGCC GAGAACAACA AGCGATTCTT TATACCTATT
      361 ATCTCAGCCT GGTATATTGG AATGGTAGTT GCTCCCGTCA ACGAGGACTA CATCCCAGAT
      421 GAGCTGTGTA AGGTGACTGG CATATCCAAG CCCATCCTGG TATTTACTAC TAGAAAGATC
      481 CTTCCAAAGG TGTTAGAGGT TAAAGACAGA ACCAACTATA TAAAGCGAAT TATAATATTA
      541 GATTCTGAGG AGAATCTACT GGGATGTGAG AGTCTCCACA ACTTTATGAG TCGTTATTCA
      601 GATAACAATC TCCAGACCTT TAAACCTTTA CACTATGATC CTGTAGATCA GGTTGCTGCC
      661 ATTCTTTGCT CTAGTGGTAC CACAGGGCTA CCAAAGGGTG TAATGCAAAC CCATAGAAAT
      721 ATCTGCGTCC GACTTACTCA TGCATCAGAT CCTAGGGTGG GGACTCAACT AATTCCTGGA
      781 GTCAGCGTAC TCGCTTATCT GCCATTCTTT CATGCATTCG GCTTTTCTAT TAATCTGGGT
      841 TATTTTATGG TGGGGCTGAG AGTCGTGATG CTACGAAGGT TTAATCAAGA AGTGTTCCTG
      901 AAAGCCATCC AAGACTATGA GGTAAGGTCT GTCATAAATG TACCTAGTAC CATTCTGTTT
      961 CTGTCTAAAT CCCCATTAGT GGACAAGTAC GACCTGTCTA CTTTGGCAGA GCTATGTTGT
     1021 GGTGCAGCAC CTTTAGCCAA GGAGGTTGCC GAGATTGCAG TCAAAAGATT AAACCTGCCA
     1081 GGAATCAGAT GTGGTTATGG GCTTACAGAG TCCACTTCCG CCAACATTCA TACTTTGCAT
     1141 AACGAGTTCA AGTCAGGTAG TTTGGGAAAA GTTACCCCCT ACATGGCCGC AAAAATAATC
     1201 GATCGTAATA CAGGCGAGGC ACTCGGACCC AACCAAGTAG GTGAGCTGTG CATTTGGGGC
     1261 CCAATGGTTA CAAAAGGCTA TGTCAACAAC CCCCAAGCAA CCAAAGAAGC AATCGACGAC
     1321 GACGGCTGGT TACATAGCGG AGACTTCGGT TACTATGATG AGGACGAATA CTTCTATATT
     1381 GTGGACCGAT ATAAGGAACT CATAAAATAC AAAGGTTATC AAGTTGCCCC TGTAGAGTTA
     1441 GAGGAGATTT TACTGCAACA CCCCGGAATA CGTGACGTTG CCGTGGTTGG CATTCCAGAT
     1501 ATTGAAGCTG GAGAACTCCC TGCTGGGTTT GTTGTCAAGC AGCCTGGCGC ACAATTGACC
     1561 GCAAAGGAGG TGTATGATTT TCTCGCCCAG CGAGTTTCAC ACTCTAAGTA TCTTCGAGGT
     1621 GGAGTCAGAT TTGTCGACTC TATCCCAAGG AACGTGACCG GGAAGATCAG TCGAAAGGAA
     1681 TTGCGTGAAG CCCTTATGGA GAAGGCATCC AAGCTCTAGC TTtgagacca cgaagtggct
     1741 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1801 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1921 gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac
     1981 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2041 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2101 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2161 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2221 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2281 ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct
     2341 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2401 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2461 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     2521 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     2581 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     2641 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     2701 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     2761 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     2821 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     2881 cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac
     2941 caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt
     3001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3241 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3841 taccgctcgc cgcagccgaa cgaccgagcg cagcga
//