LOCUS       pMC-6-AmCyan-P2A-NptII- 3796 bp ds-DNA     circular     23-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             91..777
                     /label="AmCyan"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    778..786
                     /label="GSG linker"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    787..843
                     /label="P2A"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    844..1638
                     /label="NptII"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1639..1642
                     /label="J9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1869..2879
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3031..3796
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3050..3638
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggccctgt ccaacaagtt catcggcgac
      121 gacatgaaga tgacctacca catggacggc tgcgtgaacg gccactactt caccgtgaag
      181 ggcgagggca gcggcaagcc ctacgagggc acccagacct ccaccttcaa ggtgaccatg
      241 gccaacggcg gccccctggc cttctccttc gacatcctgt ccaccgtgtt catgtacggc
      301 aaccgctgct tcaccgccta ccccaccagc atgcccgact acttcaagca ggccttcccc
      361 gacggcatgt cctacgagag aaccttcacc tacgaggacg gcggcgtggc caccgccagc
      421 tgggagatca gcctgaaggg caactgcttc gagcacaagt ccaccttcca cggcgtgaac
      481 ttccccgccg acggccccgt gatggccaag aTgaccaccg gctgggaccc ctccttcgag
      541 aagatgaccg tgtgcgacgg catcttgaag ggcgacgtga ccgccttcct gatgctgcag
      601 ggcggcggca actacagatg ccagttccac acctcctaca agaccaagaa gcccgtgacc
      661 atgcccccca accacgCggt ggagcaccgc atcgccagaa ccgacctgga caagggcggc
      721 aacagcgtgc agctCaccga gcacgccgtg gcccacatca cctccgtggt gcccttcGGA
      781 AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGTG ACGTGGAGGA GAACCCTGGA
      841 CCTgggattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
      901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gtttcggctg
      961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     1021 ctccaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     1081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     1141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     1201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     1261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     1321 gaagaacatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     1381 gacggcgagg atctcgtcgt gacacatggc gatgcctgct tgccgaatat catggtggaa
     1441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     1501 gacatagcgt tggctacccg tgatattgct gaagaacttg gcggcgaatg ggctgaccgc
     1561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     1621 cttgacgagt tcttctgaGC TTtgagacca cgaagtggct cttcagtgga cgaaagggcc
     1681 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1861 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1921 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     1981 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2041 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     2101 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2161 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2221 acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2281 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2341 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2401 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2461 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2521 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2581 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2641 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2701 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2761 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     2821 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
     2881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3181 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3781 cgaccgagcg cagcga
//