LOCUS       pMC-6-HptII-9           3274 bp ds-DNA     circular     24-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    91..1116
                     /label="Hygromycin phosphotransferase (HptII)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1117..1120
                     /label="J9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1347..2357
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2509..3274
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2528..3116
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGaaaaagc ctgaactcac cgcgacgtct
      121 gtcgagaagt ttctgatcga aaagttcgac agcgtgtccg acctgatgca gctctcggag
      181 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
      241 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
      301 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
      361 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
      421 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
      481 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
      541 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
      601 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
      661 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
      721 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
      781 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
      841 cggaggcatc cggagcttgc aggatcgcca cgactccggg cgtatatgct ccgcattggt
      901 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
      961 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     1021 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     1081 cgacgcccca gcactcgtcc gagggcaaag aaatagGCTT tgagaccacg aagtggctct
     1141 tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1201 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1261 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1321 ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg
     1381 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1441 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1501 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1561 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1621 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1681 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     1741 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1801 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1861 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1921 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1981 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2041 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2101 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2161 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2221 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2281 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     2341 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     2401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcga
//