LOCUS       pMC-6-TaGRF-GIF-9       4158 bp ds-DNA     circular     08-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J6"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    91..1311
                     /label="GRF"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             91..2001
                     /label="GRF GIF"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    1312..1323
                     /label="Alanine Linker"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1324..2001
                     /label="GIF"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2001..2004
                     /label="J9"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2231..3241
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3393..4158
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3412..4000
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa ATGGCGATGC CGTATGCCTC TCTTTCCCCG
      121 GCAGGCGACC GCCGCTCCTC CCCGGCCGCC ACCGCCACCG CCTCCCTCCT CCCaTTCTGC
      181 CGCTCCTCCC CCTTCTCCGC CGGCGGCAAT GGCGGCATGG GGGAGGAGGC GaGGATGGAC
      241 GGGAGGTGGA TGGCGAGGCC GGTGCCCTTC ACaGCGGCGC AGTACGAGGA GCTGGAGCAC
      301 CAGGCGCTCA TATACAAGTA CCTGGTGGCC GGCGTGTCCG TCCCGCCGGA TCTCGTGCTC
      361 CCaATCCGCC GCGGCATCGA GTCCCTCGCC GCCCGCTTCT ACCACAACCC CCTCGCCATC
      421 GGGTACGGAT CtTACCTGGG CAAGAAGGTG GATCCGGAGC CGGGCCGGTG CaGGCGCACG
      481 GACGGCAAGA AGTGGCGGTG CGCCAAGGAG GCCGCCTCCG ACTCCAAGTA TTGCGAGCGC
      541 CACATGCACC GCGGCCGCAA CagaTCAAGA AAGCCTGTGG AAACGCAGCT CGTgTCGCAC
      601 TCCCAGCCGC CGGCCGCCTC CGTCGTGCCG CCCCTCGCCA CCGGCTTCCA CAACCACTCC
      661 CTCTACCCaG CCATCGGCGG CACCAACGGT GGTGGAGGCG GGGGGAACAA CGGCATGTCC
      721 ATGCCCGGCA CaTTCTCCTC CGCGCTGGGG CCGCCTCAGC AGCACATGGG CAACAATGCC
      781 GCCTCTCCCT ACGCGGCTCT CGGCGGCGCC GGAACATGCA AAGATTTCAG GTATACCGCA
      841 TATGGAATAA GATCTTTGGC AGACGAGCAG AGTCAGCTCA TGACAGAAGC CATGAACACC
      901 TCCGTGGAGA ACCCATGGCG CCTGCCGCCA TCTTCTCAAA CGACTACATT CCCGCTCTCA
      961 AGCTACTCTC CTCAGCTTGG AGCAACGAGT GACCTGGGTC AGAACAACAG CAGCAACAAC
     1021 AACAGCGGCG TCAAGGCCGA GGGACAGCAG CAGCAGCAGC CGCTCTCCTT CCCGGGGTGC
     1081 GGCGACTTCG GCAGCGGCGA CTCCGCGAAG CAGGAGAACC AGACaCTGaG GCCGTTCTTC
     1141 GACGAGTGGC CaAAGACGAG GGACTCGTGG TCtGACCTGA CCGACGACAA CTCGAACGTC
     1201 GCCTCCTTCT CGGCCACCCA GCTGTCtATC TCGATACCaA TGACGTCCTC CGACTTCTCC
     1261 GCCGCCAGCT CCCAGTCGCC CAACGGCATG CTGTTCGCCG GCGAAATGTA Cgcggccgct
     1321 gccATGCAGC AGCAACACCT GATGCAGATG AACCAGAGCA TGATGGGGGG CTACGCTTCC
     1381 TCTACCACTG CCACCACTGA TCTCATTCAG CAGTACCTGG ATGAGAACAA GCAGCTGATC
     1441 CTGGCCATCC TCGACAACCA GAACAACGGC AAGGTGGAGG AGTGCGCACG GAACCAAGCT
     1501 AAGCTCCAGC AGAACCTCAT GTACCTCGCC GCCATCGCCG ACAGCCAGCC TCCGCAGACa
     1561 GCATCtCTGT CTCAGTACCC GTCCAACCTG ATGATGCAGT CCGGGCCGCG GTACATGCAG
     1621 CAGCAGTCGG CGCAGATGAT GTCGCCGCAG TCtCTGATGG CGGCGaGGTC GTCGATGATG
     1681 TACGCGCAGC AGGCCATGTC tCCGCTCCAG CAaCAGCAGC AaCAaCAGCA GCACCAGGCG
     1741 GCCGCGCACG GCCAGCTGGG GATGTCCTCC GGCGCGACCA CCGGGTTCAA CCTCCTGCAC
     1801 GGTGAGGCCA GCATGGGCGG CGGCGGCGGC GCCACTGGCA ACAGCATGAT GAACGCCAGC
     1861 GTgTTCTCGG ACTATGGCCG CGGCGGCAGC GGCGCCAAGG AGGGGTCGAC CTCGCTGTCG
     1921 GCCGACGCTC GCGGCGCCAA CTCTGGCGCG CACAGCGGCG ACGGGGAGTA CCTCAAGGGC
     1981 ACCGAGGAGG AAGGAAGCTA Gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg
     2041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2221 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2281 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2341 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2401 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     2461 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2521 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2581 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2641 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2701 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2761 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2821 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2881 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2941 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3001 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3061 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3121 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3181 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     3241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3541 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4021 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4081 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4141 aacgaccgag cgcagcga
//