LOCUS       pMC-9-OsAct2-11         2769 bp ds-DNA     circular     22-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    94..315
                     /label="3'UTR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    94..615
                     /label="OsAct2 3' UTR & terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    316..615
                     /label="Terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    612..615
                     /label="J11"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             842..1852
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2004..2769
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2023..2611
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttATGTGGC CTAGCTGTAT CTTTCATGAG
      121 AACCCCTTGT CTATTAGCAT TAGCTGGCTG TTTCATGGAC AAAAAAATTG TTGGGTCTGA
      181 TGGCTTGTCT ATGGGTGTAT CTACTCTGAT TAGCTCCGGT TTTTGTGTAT GCCAATATAA
      241 TTATTCCGTA AGTACGTGGG GAGTCAAGAA CATTATGTGT GTAGTTTGAT TTCATAAATT
      301 GATTTGAGAT TCTTGaactt ttctagtacc gcaactgcta gtctgcaacc ttgcaatgtt
      361 catcctcttt gtcatgagct tgtactactt gaagatacta tgcccctgtt tggggaggtc
      421 gagattttaa gaagtagcta gctagtgaaa atatgaaaat gctaggattc ccaaattgta
      481 gctttttgtt cattttcagg gttatataat tacatattct caaattgtcg gtatttgtat
      541 tattgggaga gctgaagatt ctaagataaa tatgatttac atcagttctc gaaaggatta
      601 cataatgggt gcgcttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
      661 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      781 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      841 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
      901 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
      961 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1021 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1081 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     1141 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     1201 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     1261 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     1321 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     1381 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     1441 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     1501 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     1561 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     1621 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     1681 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1741 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1801 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     1861 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2161 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2701 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2761 gcgcagcga
//