LOCUS       pMC-9-OsEf1a-11         2659 bp ds-DNA     circular     23-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    93..373
                     /label="3' UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    93..505
                     /label="OsEf1a 3' UTR & Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    502..505
                     /label="J11"
                     /ApEinfo_revcolor=#cc66ff
                     /ApEinfo_fwdcolor=#cc66ff
     CDS             732..1742
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1894..2659
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1913..2501
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttCCTCGTT CAAGTGGTAG TTTTGTGCAC
      121 GGCCATGGCA CTCGTTGCAT TGACCGTTAT TATCATATGC TCGCTGTGTG CACTGTGTCG
      181 TTTAAAACTC TCTATTTTCT GTTCGGTTTA AACTTGTTCT GAGCCTGATT ATTGCCCTTC
      241 GGCTGTTGTG GAATGCGGGT ATTTACATTG CTAATTCCTA TGTCTTTATT TTGGCTTGTG
      301 GCATTCGCTT GTTCTACTGC TATTGAAAAC TGTAATCTGT TTCTGTTATT AAAAACTGAA
      361 CCTTTTCTTC TGCtatttga aactgaagcg agtgggtttc aatttgctga aatcctgcta
      421 cttgctattt aaattattta tgttcaattt cgttcagtcc catatttgtt ggattgagtg
      481 aaaaactgac tactctggtt tCGCTtgaga ccacgaagtg gctcttcagt ggacgaaagg
      541 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      721 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      781 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      841 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      901 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
      961 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     1021 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     1081 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     1141 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     1201 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     1261 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     1321 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     1381 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     1441 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     1501 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1561 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1621 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1681 atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat
     1741 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1801 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1861 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1921 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1981 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2041 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2101 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2161 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2221 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2281 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2341 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2401 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2461 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2521 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2581 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2641 gaacgaccga gcgcagcga
//