LOCUS       pMC-9-PinII-11          2786 bp ds-DNA     circular     23-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    94..628
                     /label="PinII terminator (535bp variant)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    629..632
                     /label="J11"
                     /ApEinfo_revcolor=#cc66ff
                     /ApEinfo_fwdcolor=#cc66ff
     CDS             859..1869
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2021..2786
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2040..2628
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaG CTTCCCTAGA CTTGTCCATC TTCTGGATTG
      121 GCCAAGTTAA TTAAtgtATG AAATAAAAGG ATGCACACAT AGTGACATGC TAATCACTAT
      181 AATGTGGGCA TCAAagtTGT GTGTTATGTG TAATTACTAA TTATCTGAAT AAGAGAAAGA
      241 GATCATCCAT ATTTcttATC CTAAATGAAT GTCACGTGTC TTTATAATTC TTTGATGAAC
      301 CAGATGCATT TTATtaaCCA ATTCCATATA CATATAAATA TTAATCATAT ATAATTAATA
      361 TCAATTGGGT TAGCaaaACA AATCTAGTCT AGGTGTGTTT TGCTAATTAT TGGGGGATAG
      421 TGCAAAAAGA AATCtacGTT CTCAATAATT CAGATAGAAA ACTTAATAAA GTGAGATAAT
      481 TTACATAGAT TGCTtttATC CTTTGATATA TGTGAAACCA TGCATGATAT AAGGAAAATA
      541 GATAGAGAAA TAATtttTTA CATCGTTGAA TATGTAAACA ATTTAATTCA AGAAGCTAGG
      601 AATATAAATA TTGAggaGTT TATGATTACG CTtgagacca cgaagtggct cttcagtgga
      661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      841 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      901 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      961 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1021 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     1081 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1141 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     1201 ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     1261 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     1321 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     1381 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     1441 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     1501 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     1561 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     1621 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     1681 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1741 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1801 cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc
     1861 ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2161 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2701 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2761 cgcagccgaa cgaccgagcg cagcga
//