LOCUS       pMC-9-StUbi-11          2885 bp ds-DNA     circular     30-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    94..729
                     /label="StUbi terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    728..731
                     /label="J11"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    853..957
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             958..1968
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2120..2885
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2139..2727
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaG CTTGTTGCCT GTTGTTGGTT GTCGTGTTGT
      121 CTGGCTGTGT CTGTTACCCA TTGTGGTGGT TATGTGTTTG CATTATGGTC TTGAAGGACC
      181 ATCAATGTGT TTTCGCTTTC TGTTCCTTTC TGTTTCTCAT TTGTGAATAA TAATGGTGTC
      241 TTTATGAACA TCCAGTTTCT GGTTTCTTTT ATGATTGCAG TTTTGAGTTA TTTGTGTTTG
      301 CTTTTGCTTC CGTCTACTAC ACTACTTTGC AATTACTATA TCACTAATGC AGGCTTGATA
      361 TACTAAAGCC TTCGATCCAT CTTCTGTTTG ATGATTCGAA TGGTATTTAT TTAACTCATA
      421 CCCAAGTGAA GCATAAAGTT AGAGGAGAGT TCCTGTTGCA TTGCCTGTTT GTATCATGAG
      481 CAACTCATGT TAATAAACAT AAGAAAAACC ATGATGCAAT CTGTGTAGGT GATAGACTTT
      541 GATGACAGAC GGACTCATAA GTAACAAGAG ATAACAAAGA GGAAACATAA TAAACATGTA
      601 CGGGAAGTCC TCCAACAATG ACTTTAATCA CATGTTTTTG TAGATTAGCA ATTGTACATG
      661 TCAAATGATC TTGGATTAAG GATGTGAATC AAAACATCTG AATTTGGACC TAGAGTCTTG
      721 AGGTGATCGC Ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
      781 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      961 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     1021 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     1081 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     1141 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     1201 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1261 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     1321 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1381 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1441 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1501 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1561 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1621 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1681 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1741 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1801 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     1861 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     1921 tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa
     1981 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2041 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2101 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2161 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2221 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2281 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2341 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2401 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2461 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2521 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2581 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2641 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2701 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2761 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2821 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2881 agcga
//