LOCUS       pMC-6-AtSpCas9-9        6388 bp ds-DNA     circular     23-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             91..4230
                     /label="AtCas9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4195..4227
                     /label="SV40 NLS (with linker)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4207..4227
                     /label="SV40NLS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4231..4234
                     /label="J9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4461..5471
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5623..6388
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5642..6230
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaA ATGgataaga agtactctat cggactcgat
      121 atcggaacta actctgtggg atgggctgtg atcaccgatg agtacaaggt gccatctaag
      181 aagttcaagg ttctcggaaa caccgatagg cactctatca agaaaaacct tatcggtgct
      241 ctcctcttcg attctggtga aactgctgag gctaccagac tcaagagaac cgctagaaga
      301 aggtacacca gaagaaagaa caggatctgc tacctccaag agatcttctc taacgagatg
      361 gctaaagtgg atgattcatt cttccacagg ctcgaagagt cattcctcgt ggaagaagat
      421 aagaagcacg agaggcaccc tatcttcgga aacatcgttg atgaggtggc ataccacgag
      481 aagtacccta ctatctacca cctcagaaag aagctcgttg attctactga taaggctgat
      541 ctcaggctca tctacctcgc tctcgctcac atgatcaagt tcagaggaca cttcctcatc
      601 gagggtgatc tcaaccctga taactctgat gtggataagt tgttcatcca gctcgtgcag
      661 acctacaacc agcttttcga agagaaccct atcaacgctt caggtgtgga tgctaaggct
      721 atcctctctg ctaggctctc taagtcaaga aggcttgaga acctcattgc tcagctccct
      781 ggtgagaaga agaacggact tttcggaaac ttgatcgctc tctctctcgg actcacccct
      841 aacttcaagt ctaacttcga tctcgctgag gatgcaaagc tccagctctc aaaggatacc
      901 tacgatgatg atctcgataa cctcctcgct cagatcggag atcagtacgc tgatttgttc
      961 ctcgctgcta agaacctctc tgatgctatc ctcctcagtg atatcctcag agtgaacacc
     1021 gagatcacca aggctccact ctcagcttct atgatcaaga gatacgatga gcaccaccag
     1081 gatctcacac ttctcaaggc tcttgttaga cagcagctcc cagagaagta caaagagatt
     1141 ttcttcgatc agtctaagaa cggatacgct ggttacatcg atggtggtgc atctcaagaa
     1201 gagttctaca agttcatcaa gcctatcctc gagaagatgg atggaaccga ggaactcctc
     1261 gtgaagctca atagagagga tcttctcaga aagcagagga ccttcgataa cggatctatc
     1321 cctcatcaga tccacctcgg agagttgcac gctatcctta gaaggcaaga ggatttctac
     1381 ccattcctca aggataacag ggaaaagatt gagaagattc tcaccttcag aatcccttac
     1441 tacgtgggac ctctcgctag aggaaactca agattcgctt ggatgaccag aaagtctgag
     1501 gaaaccatca ccccttggaa cttcgaagag gtggtggata agggtgctag tgctcagtct
     1561 ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt gctccctaag
     1621 cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg
     1681 accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat
     1741 ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag
     1801 aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt caacgcatct
     1861 ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt ggataacgag
     1921 gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga agatagagag
     1981 atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt gatgaagcag
     2041 ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat taacggaatc
     2101 agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg attcgctaac
     2161 agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga tatccagaag
     2221 gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct cgctggatct
     2281 cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt ggtgaaggtg
     2341 atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa ccagaccact
     2401 cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg tatcaaagag
     2461 cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca gaatgagaag
     2521 ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga gttggatatc
     2581 aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt gaaggatgat
     2641 tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag tgataacgtg
     2701 ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct caacgctaag
     2761 ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg actctctgaa
     2821 ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat cactaagcac
     2881 gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga taagttgatc
     2941 agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag aaaggatttc
     3001 caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc ttaccttaac
     3061 gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga gttcgtgtac
     3121 ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca agagatcgga
     3181 aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa gaccgagatt
     3241 accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg tgaaacaggt
     3301 gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct ctctatgcca
     3361 caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa agagtctatc
     3421 ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga ccctaagaaa
     3481 tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc taaggttgag
     3541 aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac tatcatggaa
     3601 aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata caaagaggtt
     3661 aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga gaacggtaga
     3721 aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc tctcccatct
     3781 aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg atctccagaa
     3841 gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga tgagatcatc
     3901 gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct cgataaggtg
     3961 ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga gaacatcatc
     4021 catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt cgatacaacc
     4081 atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct catccatcag
     4141 tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg tgattcaagg
     4201 gctgatccta agaagaagag gaaggtttga GCTTtgagac cacgaagtgg ctcttcagtg
     4261 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4321 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4381 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4441 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     4501 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     4561 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     4621 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     4681 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     4741 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     4801 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     4861 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     4921 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     4981 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     5041 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     5101 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     5161 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     5221 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     5281 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     5341 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     5401 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
     5461 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5761 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6361 gccgcagccg aacgaccgag cgcagcga
//