LOCUS       pMC-1-AtU6-Y            2692 bp ds-DNA     circular     23-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     promoter        94..538
                     /label="AtU6 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    535..538
                     /label="Junction Y"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    660..764
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             765..1775
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1927..2692
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1946..2534
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcttcgtt gaacaacgga aactcgactt
      121 gccttccgca caatacatca tttcttctta gctttttttc ttcttcttcg ttcatacagt
      181 ttttttttgt ttatcagctt acattttctt gaaccgtagc tttcgttttc ttctttttaa
      241 ctttccattc ggagtttttg tatcttgttt catagtttgt cccaggatta gaatgattag
      301 gcatcgaacc ttcaagaatt tgattgaata aaacatcttc attcttaaga tatgaagata
      361 atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag
      421 aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca
      481 tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag tagtgatttg
      541 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
      601 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
      781 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
      841 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
      901 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
      961 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1021 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1081 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     1141 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1201 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1261 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1321 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1381 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1441 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1501 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1561 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1621 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1681 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     1741 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     1801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2581 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2641 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc ga
//