LOCUS       pMC-1-OsU6-X            2489 bp ds-DNA     circular     23-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     promoter        93..335
                     /label="OsU6 promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    332..335
                     /label="Junction X"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    457..561
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             562..1572
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1724..2489
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1743..2331
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gaggatcatg aaccaacggc ctggctgtat
      121 ttggtggttg tgtagggaga tggggagaag aaaagcccga ttctcttcgc tgtgatgggc
      181 tggatgcatg cgggggagcg ggaggcccaa gtacgtgcac ggtgagcggc ccacagggcg
      241 agtgtgagcg cgagaggcgg gaggaacagt ttagtaccac attgcccagc taactcgaac
      301 gcgaccaact tataaacccg cgcgctgtcg cttgttgaga ccacgaagtg gctcttcagt
      361 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      541 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      601 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
      661 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      721 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
      781 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
      841 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
      901 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
      961 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     1021 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     1081 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     1141 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     1201 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     1261 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     1321 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     1381 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     1441 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     1501 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     1561 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1621 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1681 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1741 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1801 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1861 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1921 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1981 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2041 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2101 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2161 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2221 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2281 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2341 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2401 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2461 cgccgcagcc gaacgaccga gcgcagcga
//