LOCUS       pJMC-5T                 3487 bp ds-DNA     circular     24-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    41..44
                     /label="M5"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    53..56
                     /label="J1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    63..850
                     /label="Chloramphenicol selection cassette"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    86..116
                     /label="Lac UV5 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    170..850
                     /label="Chloramphenicol acetyltransferase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    895..1475
                     /label="CcdB selection cassette"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    948..1133
                     /label="CcdA (2bp insertion mutant)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1170..1475
                     /label="CcdB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1483..1486
                     /label="J11"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1493..1496
                     /label="M omega"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1553..1673
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1640..1655
                     /label="duplication"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1674..2534
                     /label="AmpR marker: class A beta-lactamase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2722..3487
                     /label="pMB1-derived ORI fragment"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2741..3329
                     /label="pMB1-derived ORI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgcTTTT CCTCggcgcg ccGGAGtGAG
       61 ACcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      121 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      241 atttcagtca gtcgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
      421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      601 cgtTtcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
      721 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
      781 gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc tagaggatcc
      841 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
      901 atatatactg atatgtatac ccgaagtatg tcaaaaggag gtatgctatg aagcagcgta
      961 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     1021 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     1081 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     1141 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     1201 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     1261 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     1321 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     1381 tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     1441 aaaacgccat taacctgatg ttctggggaa tataaGGTCT CtCGCTgagc tcAGTGTTTT
     1501 gcaggtgTGa tgaaaagcct tagggtcagg tggcactttt cggggaaatg tgcgatgaac
     1561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctaatga gacaataacc
     1621 ctgataaatg cttcaattaT ATGAAAAAGG AAGAGtattg aaaaaggaag agtatgagta
     1681 tccaacattt ccgtgtcgcc cttattccct tttttgcggc cttctgcctt cctgtttttg
     1741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcccgagtgg
     1801 gttacattga actggatctc aacagcggca aaatcttaga gagttttcgc cccgaagaac
     1861 gcttcccaat gatgagcact ttcaaagttc tgctatgtgg cgcggtgtta tcccgtattg
     1921 atgctgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1981 attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgtagtg
     2041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac
     2101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2221 caatggcaac aactctccgc aaactattaa ctggcgaact acttactctt gcttcacgcc
     2281 agcaactcat tgactggatg gaggcggaca aagttgcagg accacttctg cgctcggcac
     2341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggC tctcgcggta
     2401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2521 ttaagcattg gtaactgtca gacgaaggga aataaatagt agcccgcctg atgtgcgggc
     2581 ttttttttgg tggtggttac cagtgtcgct tagctaccgt aggtcctctt aggcggtccg
     2641 attagcgaca tagtcatcaa tgccttgacg gcaaaatccc ttaacgtgag ttttcgttcc
     2701 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2761 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2821 atcaagagct actaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2881 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2941 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3001 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3061 cggggggttc gtgctcacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3121 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3181 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3241 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3301 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3361 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3421 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3481 gcagcga
//